Mouse platelet activating factor,PAF ELISA KIT

Code CSB-E08199m
Size 96T,5×96T,10×96T
Price Request a Quote
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Product Details

Target Name
platelet activating factor,PAF
Alternative Names
N/A
Abbreviation
PAF
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.78 ng/mL-50 ng/mL
Sensitivity
0.195 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Biochemicals
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 86-98  
1:2 Average % 97  
Range % 92-107  
1:4 Average % 93  
Range % 87-98  
1:8 Average % 87  
Range % 82-94  
Recovery
The recovery of mouse PAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 87-97  
EDTA plasma (n=4) 97 95-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
50 2.576 2.652 2.614 2.460  
25 2.044 2.076 2.060 1.906  
12.5 1.443 1.504 1.474 1.320  
6.25 0.696 0.750 0.723 0.569  
3.12 0.466 0.504 0.485 0.331  
1.56 0.354 0.369 0.362 0.208  
0.78 0.241 0.259 0.250 0.096  
0 0.153 0.155 0.154    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

Platelet activating factor (PAF) is a potent phospholipid mediator that plays key roles in inflammation, immune responses, and cardiovascular physiology. This bioactive lipid molecule participates in platelet aggregation, neutrophil activation, and vascular permeability regulation. PAF signaling contributes to various pathological processes including allergic reactions, sepsis, and ischemia-reperfusion injury. This makes it an important research target for understanding inflammatory diseases and developing therapeutic interventions.

The Mouse platelet activating factor PAF ELISA Kit (CSB-E08199m) provides quantitative measurement of PAF in Mus musculus samples using a sandwich assay principle. This kit accommodates serum, plasma, cell culture supernatants, and tissue homogenates with a detection range of 0.78-50 ng/mL and sensitivity of 0.195 ng/mL. The assay requires 50-100 μL sample volume, completes within 1-5 hours, and uses detection at 450 nm wavelength for reliable quantification of mouse PAF levels.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in research examining inflammatory processes and biomarker profiling in mouse models. Studies have applied the kit to measure platelet activating factor levels in various biological samples. These include plasma and tissue homogenates from hippocampal tissues.

Neuroinflammation research: Measurement of platelet activating factor levels in hippocampal tissue homogenates and plasma samples in behavioral studies involving animal models
Inflammatory biomarker profiling: Quantification of platelet activating factor alongside other inflammatory mediators in plasma samples from mouse studies
Tissue-specific analysis: Measurement of platelet activating factor in brain tissue preparations following behavioral testing protocols

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