Mouse suppressors of cytokine signaling 1(SOCS-1)ELISA Kit

Instructions
Code CSB-E17346m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name suppressors of cytokine signaling 1(SOCS-1)
Alternative Names Socs1 ELISA Kit; Cish1 ELISA Kit; Ssi1 ELISA Kit; Suppressor of cytokine signaling 1 ELISA Kit; SOCS-1 ELISA Kit; JAK-binding protein ELISA Kit; JAB ELISA Kit; STAT-induced STAT inhibitor 1 ELISA Kit; SSI-1 ELISA Kit
Abbreviation SOCS1
Uniprot No. O35716
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SOCS-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 84-94  
1:2 Average % 98  
Range % 93-102  
1:4 Average % 92  
Range % 88-97  
1:8 Average % 95  
Range % 89-99  
Recovery
The recovery of mouse SOCS-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 86-95  
EDTA plasma (n=4) 99 94-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.482 2.452 2.467 2.328  
2000 1.976 2.071 2.024 1.885  
1000 1.604 1.663 1.634 1.495  
500 1.195 1.139 1.167 1.028  
250 0.624 0.614 0.619 0.480  
125 0.439 0.416 0.428 0.289  
62.5 0.229 0.246 0.238 0.099  
0 0.141 0.137 0.139    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity (By similarity). Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival. Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2 (By similarity). SOCS1 appears to be a negative regulator in IGF1R signaling pathway (By similarity).
Gene References into Functions
  1. The present work shows the role of SOCS1 in murine melanoma development and the potential of SOCS1-silenced tumor cells in raising an effective anti-melanoma immune response. PMID: 28079159
  2. Feeding Guar gum fiber increases SOCS-1, TLR2 and dectin-1 expression in dextran sodium sulfate administered and normal mice. PMID: 28608623
  3. SOCS1 attenuates migration and invasion properties of hepatocellular carcinoma cells at least partly via modulation of MET-mediated epithelial-mesenchymal transition, and controls invasive tumor growth. PMID: 29085209
  4. Downregulated SOCS1 expression activates the JAK1/STAT1 pathway and promotes polarization of macrophages into M1 type. PMID: 28901399
  5. these findings suggest that SOCS-1 may exert its protective effect in acute lung injury by rescuing epithelial sodium channel alpha-subunit expression via suppression of ASK-1. PMID: 27058411
  6. loss of SOCS1 in CD11c+ cells skewed the balance of immune response to infection by increasing innate responses while decreasing antigen-specific adaptive responses to infectious antigens PMID: 29150567
  7. MiRNA sponges against miR-19 or miR-155 inhibit the functions of these miRNAs and potentiate the induction of SOCS1 and p53 in mouse leukemia cells and in human myeloma cells. PMID: 26841929
  8. IFN-lambda signaling is regulated by SOCS1 but not by SOCS3 or USP18. PMID: 28900038
  9. Study conclude that under pro-proliferative cytokine stimulation at the onset of myeloproliferative diseases SOCS1 acts as a tumor suppressor, while under anti-proliferative conditions it exerts oncogenic function. PMID: 28753604
  10. results indicate that SOCS1 is one of the essential molecules that maintain regulatory T cell stability, particularly under the inflammatory conditions in which APCs are highly activated PMID: 28550203
  11. The Stat3 exerts its activity through the induction of miR- 155, which suppresses suppressor of cytokine signaling (SOCS)1. PMID: 27183599
  12. miR-155 not only directly inhibited SOCS1 expression, but also increased the expression of p-STAT and PDCD4, as well as the production of proinflammation mediators IL-6 and TNF-alpha in atherogenesis. PMID: 27843203
  13. TLR8 coupling with SOCS-1 inhibits TLR7-mediated antiviral immunity during WNV infection in mice. PMID: 27798161
  14. nectin-4 serves as a stimulatory co-receptor for the prolactin receptor by regulating the feedback inhibition of SOCS1 in the JAK2-STAT5a signaling pathway PMID: 28258213
  15. this study shows that genetic modification of dendritic cells with SOCS1 affects their activation and maturation, inhibits T-cell proliferation and induces hypo-responsiveness, and prolongs islet allograft survival PMID: 28215626
  16. SOCS1 may promote apoptosis of intestinal epithelial cells at least partly through mediating p53 signaling. PMID: 27236107
  17. Kdm5a associates with p50 and binds to the Socs1 promoter region in resting natural killer cells. PMID: 27050510
  18. The miR-155 inhibitor relieves liver injury by enhancing the expression of SOCS1 and inactivating JAK/STAT signaling. PMID: 26251957
  19. NOS1-derived nitric oxide promotes NF-kappaB transcriptional activity through inhibition of Socs1.Cys147 and Cys179 are essential for SOCS1 nitrosation and proteasomal degradation. PMID: 26324446
  20. mir-155 deficiency attenuates liver ischemia-reperfusion injury through upregulation of SOCS1, which is associated with promotion of M2 macrophage and inhibition of Th17 differentiation PMID: 25611689
  21. Findings indicate that SOCS1 mediates its tumor suppressor functions, at least partly, by binding to MET and interfering with downstream signaling pathways as well as by promoting the turnover of the activated MET receptor. PMID: 25728680
  22. increased expression of SOCS-1 in the lungs of mice significantly protects against hyperoxic lung injury. PMID: 25850028
  23. Overexpression of SOCS1 in transgenic rat eyes attenuated ocular HSV-1 infection PMID: 24993154
  24. negative regulator of cell differentiation and phagocytosis in bone marrow-derived dendritic cells PMID: 25381480
  25. Axl promotes STAT1 signaling via inhibition of SOCS1 in activated smooth muscle cells in vein graft remodeling. PMID: 26276821
  26. targeting SOCS proteins, predominantly SOCS1, to suppress pathological mechanisms involved in atheroma plaque progression and destabilization could be an interesting anti-atherosclerotic strategy PMID: 25604439
  27. While SOCS1 expression in adaptive immune cells is sufficient to inhibit antiviral immunity, SOCS1 in innate/stromal cells is responsible for aggravated lung injury. PMID: 25500584
  28. miR-155 controls the outcome of the the germinal center reaction by modulating its initiation (Aicda) and termination (Socs1/p53 response). PMID: 25645925
  29. these data imply that suppression of IFN-lambda signaling by virus-induced SOCS-1 causes an adaptive increase in IFN-lambda expression by host to protect cells against the viral infection PMID: 24391501
  30. The Socs1 suppresses tumor formation in mammary glands by preventing the natural developmental function of a growth factor signaling pathway from becoming pro-oncogenic. PMID: 24037089
  31. This study is the first to provide evidence that SOCS1 is involved in the regulation of neurogenesis. PMID: 24557021
  32. These data demonstrate an important role for miR-155 in promoting staphylococcal enterotoxin b induced inflammation in the lungs through Socs1 suppression. PMID: 24778118
  33. SOCS1 prevents GA progression by preserving endothelial function and attenuating cytokine-induced adhesion molecule expression in vascular endothelium. PMID: 23994402
  34. These findings underscore the importance of SOCS1 in regulating potentially skin-reactive cytotoxic T lymphocytes, which could get activated under conditions that promote Ag-nonspecific, cytokine-driven proliferation. PMID: 23443260
  35. These data indicate that IL-30 can provide critical protection against macrophage-mediated liver inflammation through anti-apoptotic, anti-oxidant, and anti-inflammatory activities PMID: 23583238
  36. SOCS1 expression in DCs was down-regulated by 80% after Len-SOCS1-shRNA lentivirus infection. PMID: 23643168
  37. Loss of SOCS-1 in the low-density lipoprotein receptor deficient murine model of atherosclerosis resulted in a complex, systemic and ultimately lethal inflammation. PMID: 23300554
  38. miR-155 enhanced Treg and Th17 cells differentiation and IL-17A production by targeting SOCS1. PMID: 23091595
  39. Progesterone suppresses TLRs-triggered immune response by regulating miR-155. The decreased miR-155 contributes to inhibit TLR-induced IL-6 and IFN-beta via increased SOCS1 expression. PMID: 22546503
  40. miR-155 modulates TNF-alpha-regulated osteogenic differentiation by targeting SOCS1. PMID: 22634176
  41. miR-155, an IFN-beta-induced miRNA, mediated the suppressive effect of IFN-beta on osteoclast differentiation by targeting SOCS1 and MITF, two essential regulators of osteoclastogenesis PMID: 22771905
  42. High level of IL-10 within tumour microenvironment compromises Socs1-silenced dendritic cell vaccine functions. PMID: 22230748
  43. Socs1 accelerates the onset of Flt3-internal tandem duplication - induced myeloproliferative disease by promoting the escape from external cytokine control. PMID: 22517899
  44. Our findings suggest that SOCS1 DNA administration has considerable therapeutic potential in individuals with autoimmune myocarditis and dilated cardiomyopathy PMID: 22798678
  45. SOCS1 is expressed via a new, NF-kappaB-independent pathway in Dectin-1-triggered murine bone marrow macrophages and influences Toll-like receptor cross-talk and T cell priming. PMID: 22547696
  46. Toll-like receptor 2-mediated signalling is negatively regulated by SIGNR1-mediated signalling in dendritic cells, possibly through suppressor of cytokine signalling-1-mediated degradation of the MyD88-adaptor-like protein. PMID: 21899704
  47. Socs1(-/-)Ifng(-/-) mice displayed accelerated liver regeneration with increased DNA synthesis compared to Ifng(-/-) and wild type mice. PMID: 21703184
  48. results exemplify the negative role played by SOCS1 during T cell priming and effector functions during M. tuberculosis infection PMID: 21537342
  49. miR-155 had a pro-inflammatory role in microglia and was necessary for the progression of the immune response through the modulation of SOCS-1. PMID: 22043967
  50. SOCS1 is not only a feedback inhibitor of inflammation but also a critical molecular switch that tunes key signaling pathways to effectively program different sides of the macrophage balance PMID: 21628332

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Subcellular Location Nucleus, Cytoplasmic vesicle
Protein Families SOCS1 family
Tissue Specificity High expression in thymus. Lower expression in lung and spleen. Expressed in both Th1 and Th2 cells.
Database Links

KEGG: mmu:12703

STRING: 10090.ENSMUSP00000038121

UniGene: Mm.130

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