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Pig Interleukin 1β,IL-1β ELISA Kit

Citations (33) Protocol MSDS
Code CSB-E06782p
Size 96T,5×96T,10×96T
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Product Details

Target Name
interleukin 1, beta
Associated names
IL1BInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit
Abbreviation
IL1B
Uniprot No.
P26889
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
3.9 pg/mL-250 pg/mL
Sensitivity
0.975 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %88
Range %80-100
1:2Average %96
Range %91-103
1:4Average %95
Range %90-110
1:8Average %95
Range %85-105
Recovery
The recovery of pig IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9583-104
EDTA plasma (n=4)9890-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
2502.675 2.815 2.745 2.597
1252.154 2.147 2.151 2.003
62.51.312 1.335 1.324 1.176
31.250.788 0.764 0.776 0.628
15.60.377 0.356 0.367 0.219
7.80.264 0.269 0.267 0.119
3.90.190 0.188 0.189 0.041
00.153 0.143 0.148 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Pig IL1B ELISA Kit was designed for the quantitative measurement of Pig IL1B protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.9 pg/mL-250 pg/mL and the sensitivity is 0.975 pg/mL .

Citations

Related Products

IL1B Proteins

Customer Reviews and Q&A

■ Customer Reviews
Average Rating:
5.0 - 2 reviews

Submit a Review here

Sample type: Cell culture supernatant

Sample species: Pig

Sample dilution: No dilution

Review: The results are very satisfactory. I used the cell culture medium supernatant, and the other kits had interfering factors, but the CUSABIO did not, and after trying several other kits, I found that this brand was the only one that worked well.

By Anonymous

Sample type: Serum

Sample species: Pig

Sample dilution: : 1:10

Review: We evaluated the establishment of the LPS-induced piglet model by detecting the content of this indicator in the blood and found that the indicator in the blood of piglets increased significantly after LPS induction.

By Anonymous

Read more reviews >>
■ Q&A
Q:

Hello, please advise if the Pig IL-1b ELISA kit (#CSB-E06782p) recognizes only mature IL-1b or both mature and pro-IL-1b protein. Thank you.

A:
Thanks for your inquiry. This kit detects total IL-1b, including mature form and pro form.

Target Background

Function
(From Uniprot)
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
Gene References into Functions
  1. Analysis of host-protein interactions for African swine fever virus L83L identified IL-1beta as its host ligand. A recombinant African swine fever virus lacking the L83L was developed from the highly virulent field isolate Georgia2007 and was used to show that L83L is a non-essential gene. PMID: 29605728
  2. This study showed that the presence of embryos in oviducts on days 2-3 after mating may influence the oviductal expression of the members of the IL-1beta system, determining the action of IL-1beta, which may be considered to be the earliest sign of pregnancy in pigs. PMID: 28532837
  3. This study showed that classical swine fever virus and p7 protein induced IL-1beta secretion and that p7 protein was a short-lived protein degraded by the proteasome. PMID: 25146005
  4. local expression of IL-1beta and IL-8 in non-bacterial thrombotic endocarditis, Staphylococcus aureus infective endocarditis, animals with S. aureus sepsis without endocarditis and controls PMID: 23879680
  5. IL1B regulates expression of IL1R1 and IL1RAP and stimulates expression of PTGS1 and PTGS2 that are considered to be the most rate-limiting enzymes for endometrial synthesis of prostaglandins during the peri-implantation period of pregnancy in pigs. PMID: 22572995
  6. the results presented here strongly suggest IL-1beta as a key molecule guiding tissue remodelling events after myocardial infarction. PMID: 20678474

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Subcellular Location
Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
Protein Families
IL-1 family
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Related pathways
MAPK signaling pathway Lipids and Inflammation in Atherogenesis NF-kappa B signaling pathway TNF signaling pathway Necroptosis Hematopoietic cell lineage IL-17 signaling pathway Inflammasome Signaling NOD-like receptor signaling pathway Osteoclast differentiation Th17 cell differentiation Toll-like receptor signaling pathway AGE-RAGE signaling pathway in diabetic complications Excitatory synapse pathway
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