Rabbit Interleukin 1β,IL-1β ELISA Kit

Instructions
Code CSB-E06900Rb
Size 96T,5×96T,10×96T
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Product Details

Target Name interleukin 1, beta
Alternative Names IL1BInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit; Lymphocyte proliferation-potentiating factor ELISA kit
Abbreviation IL1B
Uniprot No. P14628
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, tissue homogenates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %98
Range %85-105
1:2Average %95
Range %85-103
1:4Average %94
Range %82-100
1:8Average %95
Range %85-105
Recovery
The recovery of rabbit IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9682-104
EDTA plasma (n=4)9585-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
5002.852 2.713 2.783 2.674
2502.235 2.245 2.240 2.131
1251.424 1.392 1.408 1.299
62.50.753 0.784 0.769 0.660
31.20.447 0.433 0.440 0.331
15.60.266 0.282 0.274 0.165
7.80.202 0.195 0.199 0.090
00.111 0.106 0.109  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.
Gene References into Functions
  1. Mild heat shock increased the production of inflammatory cytokines, IL-1beta and IL-6 in rabbit cornea cells. PMID: 23330884
  2. IL-1beta and TNF-alpha expression increases significantly during acute lung injury. Ambroxol combined with low-dose heparin inhibits teh release of IL-1beta and TNF-alpha. PMID: 21845882
  3. IL-1beta induced a significant reduction in the relative intrinsic activity of GLUT5 and in this decrease are involved NO signal pathways; blockage of D-fructose intestinal uptake by IL-1beta may play an essential role in the pathophysiology of septic shock. PMID: 20803526
  4. The inhibitory effect of IL-1beta on D-galactose absorption across mucosal side of enterocyte could be mediated by the activation of several kinases and nuclear factor kappa B. PMID: 22759965
  5. Glucosamine hydrochloride treatment can can partially decrease the expression levels of IL-1 beta and increase the expression levels of TGF-beta 1, which delays the development of osteoarthritis. PMID: 20369526
  6. In this study evidence is provided that exogenous PGF2alpha differentially modulates luteal expression of IL1B transcripts depending on luteal stage. PMID: 20427144
  7. results revealed that a transient episode of raised-intensity phonation causes a significant increase in vocal fold inflammatory mRNA expression - IL-1beta,COX-2, and TGFbeta1 PMID: 20879195

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Subcellular Location Cytoplasm, cytosol, Lysosome, Secreted, exosome, Cytoplasmic vesicle, autophagosome, Secreted
Protein Families IL-1 family
Database Links

KEGG: ocu:100008990

STRING: 9986.ENSOCUP00000021869

UniGene: Ocu.1993

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