Rat Apoptosis regulator BAX(BAX) ELISA kit

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Rat Apoptosis regulator BAX(BAX) ELISA kit

Name: Rat Apoptosis regulator BAX(BAX) ELISA kit
Brand: CUSABIO
SKU: CSB-EL002573RA

^Instructions

Code	CSB-EL002573RA
Size	96T,5×96T,10×96T
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Target Data

Target Name

Apoptosis regulator BAX(BAX)

Alternative Names

BCL2L4, apoptosis regulator BAX

Abbreviation

BAX

Uniprot No.

Q63690

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat BAX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-92
1:2	Average %	96
	Range %	91-105
1:4	Average %	90
	Range %	87-99
1:8	Average %	93
	Range %	82-98

Recovery

The recovery of rat BAX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-97
EDTA plasma (n=4)	97	93-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.091	1.995	2.043	1.844
2000	1.751	1.776	1.764	1.565
1000	1.368	1.432	1.400	1.201
500	0.985	0.972	0.979	0.780
250	0.535	0.544	0.540	0.341
125	0.348	0.352	0.350	0.151
62.5	0.244	0.237	0.241	0.042
0	0.201	0.197	0.199

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Effect of maternal omega-3 fatty acids and vitamin E supplementation on placental apoptotic markers in rat model of early and late onset preeclampsia Vaishali Kasture, et al,Life Sciences,2019
Oleuropein suppresses oxidative, inflammatory, and apoptotic responses following glycerol-induced acute kidney injury in rats Yin M, et al,Life Sciences,2019
Nephroprotective Role of Selenium Nanoparticles Against Glycerol-Induced Acute Kidney Injury in Rats AlBasher G, et al,Biological Trace Element Research,2019
Ameliorative Effect of Beta vulgaris Root Extract on Chlorpyrifos-Induced Oxidative Stress, Inflammation and Liver Injury in Rats Albasher G, et al,Biomolecules,2019
Sevoflurane reduces ischemic brain injury in rats with diet and streptozotocin-induced diabetes Zhang H,Journal of Receptors and Signal Transduction,2019
The Cardioprotective Effects of Remote Ischemic Conditioning in a Rat Model of Acute Myocardial Infarction You L, et al,Medical science monitor,2019
Olmesartan ameliorates chemically-induced ulcerative colitis in rats via modulating NFκB and Nrf-2/HO-1 signaling crosstalk Saber S, et al,Toxicology and Applied Pharmacology,2018
Epigallocatechin-3-gallate protects against diabetic cardiomyopathy through modulating the cardiometabolic risk factors, oxidative stress, inflammation, cell death and fibrosis in streptozotocin-nicotinamide-induced diabetic rats Othman A.et al,Biomed Pharmacother.,2017
Effect of Ukrain on Ischemia/Reperfusion-Induced Kidney Injury in Rats Aras B.et al,J Surg Res.,2016
Effect of ukrain on ischemia/reperfusion-induced kidney injury in rats Bekir Aras. et al,Journal of Surgical Research,2015

Function	Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Subcellular Location	Isoform Alpha: Mitochondrion outer membrane, Single-pass membrane protein, Cytoplasm
Protein Families	Bcl-2 family
Tissue Specificity	Expressed in a wide variety of tissues, with highest levels in the testis and ovary.
Database Links	STRING: 10116.ENSRNOP00000028328 UniGene: Rn.10668

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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