Rat Apoptosis regulator BAX(BAX) ELISA kit

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Rat Apoptosis regulator BAX(BAX) ELISA kit

Citations (27) Protocol

Code	CSB-EL002573RA
Size	96T,5×96T,10×96T How to order?
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Citations (27)
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Description

This Rat BAX ELISA Kit was designed for the quantitative measurement of Rat BAX protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

Target Name

Apoptosis regulator BAX(BAX)

Alternative Names

Bax; Apoptosis regulator BAX

Abbreviation

BAX

Uniprot No.

Q63690

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat BAX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-92
1:2	Average %	96
	Range %	91-105
1:4	Average %	90
	Range %	87-99
1:8	Average %	93
	Range %	82-98

Recovery

The recovery of rat BAX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-97
EDTA plasma (n=4)	97	93-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.091	1.995	2.043	1.844
2000	1.751	1.776	1.764	1.565
1000	1.368	1.432	1.400	1.201
500	0.985	0.972	0.979	0.780
250	0.535	0.544	0.540	0.341
125	0.348	0.352	0.350	0.151
62.5	0.244	0.237	0.241	0.042
0	0.201	0.197	0.199

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Icariin promotes mouse Leydig cell testosterone synthesis via the Esr1/Src/Akt/Creb/Sf-1 pathway J Sun,Toxicology and Applied Pharmacology,2022
Apigenin attenuates molecular, biochemical, and histopathological changes associated with renal impairments induced by gentamicin exposure in rats Y Xie,ResearchSquare,2022
The Effect of Continuous Aerobic Training on Bax/Bcl2 Ratio in Pancreatic Tissue Diabetic Rats M Bostani,/,2021
Selenium nanoparticles to rescue renal damage in rats JKJ imre as Latest,/,2021
Using Green Biosynthesized Lycopene-Coated Selenium Nanoparticles to Rescue Renal Damage in Glycerol-Induced Acute Kidney Injury in Rats A Al-Brakati,International Journal of Nanomedicine,2021
Octreotide and melatonin alleviate inflammasome-induced pyroptosis through inhibiting TLR4-NF-κB-NLRP3 pathway in hepatic ischemia/reperfusion injury AEDES El,Toxicology and Applied Pharmacology,2020
Moringa oleifera Lam. extract rescues lead‐induced oxidative stress, inflammation, and apoptosis in the rat cerebral cortex WS Alqahtani,Journal of food biochemistry,2020
Oxidative stress-mediated induction of pulmonary oncogenes, inflammatory, and apoptotic markers following time-course exposure to ethylene glycol monomethyl ether in rats Oluwatobi T. Somade,Metabolism Open,2020
The interrupted cross-talk of inflammatory and oxidative stress trajectories signifies the effect of artesunate against hepatic ischemia/reperfusion-induced inflammasomopathy MES Ghoneim,Toxicology and Applied Pharmacology,2020
Hepatic oxidative stress, up-regulation of pro-inflammatory cytokines, apoptotic and oncogenic markers following 2-methoxyethanol administrations in rats OT Somade,Biochemistry and Biophysics Reports,2020
Ethylene glycol monomethyl ether-induced testicular oxidative stress and time-dependent up-regulation of apoptotic, pro-inflammatory, and oncogenic markers in rats OT Somade,Metabolism Open,2020
Protective effects of Artemisia judaica extract compared to metformin against hepatorenal injury in high-fat diet/streptozotocine-induced diabetic rats G Albasher,Environmental Science and Pollution Research,2020
Methyl cellosolve-induced renal oxidative stress and time-dependent up-regulation of pro-inflammatory cytokines, apoptotic, and oncogenic markers in rats OT Somade,Toxicology Reports,2020
Impact of Coenzyme Q10 Administration on Lead Acetate-Induced Testicular Damage in Rats M El-khadragy,Oxid Med Cell Longev,2020
Investigation of antioxidant and anti-inflammatory activities as well as the renal protective potential of green coffee extract in high fat-diet/streptozotocin-induced diabetes in male albino rats OD AlAmri,Journal of Functional Foods,2020
Neuroprotective role of luteolin against lead acetate-induced cortical damage in rats Baty RS,Hum Exp Toxicol,2020
Antagonistic Efficacy of Luteolin against Lead Acetate Exposure-Associated with Hepatotoxicity is Mediated via Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Activities AL-Megrin W A,Antioxidants,2019
Effect of maternal omega-3 fatty acids and vitamin E supplementation on placental apoptotic markers in rat model of early and late onset preeclampsia Vaishali Kasture, et al,Life Sciences,2019
Oleuropein suppresses oxidative, inflammatory, and apoptotic responses following glycerol-induced acute kidney injury in rats Yin M, et al,Life Sciences,2019
Nephroprotective Role of Selenium Nanoparticles Against Glycerol-Induced Acute Kidney Injury in Rats AlBasher G, et al,Biological Trace Element Research,2019
Ameliorative Effect of Beta vulgaris Root Extract on Chlorpyrifos-Induced Oxidative Stress, Inflammation and Liver Injury in Rats Albasher G, et al,Biomolecules,2019
Sevoflurane reduces ischemic brain injury in rats with diet and streptozotocin-induced diabetes Zhang H,Journal of Receptors and Signal Transduction,2019
The Cardioprotective Effects of Remote Ischemic Conditioning in a Rat Model of Acute Myocardial Infarction You L, et al,Medical science monitor,2019
Olmesartan ameliorates chemically-induced ulcerative colitis in rats via modulating NFκB and Nrf-2/HO-1 signaling crosstalk Saber S, et al,Toxicology and Applied Pharmacology,2018
Epigallocatechin-3-gallate protects against diabetic cardiomyopathy through modulating the cardiometabolic risk factors, oxidative stress, inflammation, cell death and fibrosis in streptozotocin-nicotinamide-induced diabetic rats Othman A.et al,Biomed Pharmacother.,2017
Effect of Ukrain on Ischemia/Reperfusion-Induced Kidney Injury in Rats Aras B.et al,J Surg Res.,2016
Effect of ukrain on ischemia/reperfusion-induced kidney injury in rats Bekir Aras. et al,Journal of Surgical Research,2015

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Function (From Uniprot)	Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Subcellular Location	[Isoform Alpha]: Mitochondrion outer membrane; Single-pass membrane protein. Cytoplasm.; [Isoform Beta]: Cytoplasm.; [Isoform Gamma]: Cytoplasm.
Protein Families	Bcl-2 family
Tissue Specificity	Expressed in a wide variety of tissues, with highest levels in the testis and ovary.
Database Links	STRING: 10116.ENSRNOP00000028328 UniGene: Rn.10668

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Related pathways

p53 signaling pathway Apoptosis Protein processing in endoplasmic reticulum Necroptosis AGE-RAGE signaling pathway in diabetic complications Neurotrophin signaling pathway

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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