Rat Apoptosis regulator BAX(BAX) ELISA kit

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Target Data

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Rat Apoptosis regulator BAX(BAX) ELISA kit

Name: Rat Apoptosis regulator BAX(BAX) ELISA kit
Brand: CUSABIO
SKU: CSB-EL002573RA

^Instructions

Code	CSB-EL002573RA
Size	96T,5×96T,10×96T
Trial Size
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Citations
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Target Data

Target Name

Apoptosis regulator BAX(BAX)

Alternative Names

BaxApoptosis regulator BAX ELISA kit

Abbreviation

BAX

Uniprot No.

Q63690

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat BAX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-92
1:2	Average %	96
	Range %	91-105
1:4	Average %	90
	Range %	87-99
1:8	Average %	93
	Range %	82-98

Recovery

The recovery of rat BAX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-97
EDTA plasma (n=4)	97	93-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.091	1.995	2.043	1.844
2000	1.751	1.776	1.764	1.565
1000	1.368	1.432	1.400	1.201
500	0.985	0.972	0.979	0.780
250	0.535	0.544	0.540	0.341
125	0.348	0.352	0.350	0.151
62.5	0.244	0.237	0.241	0.042
0	0.201	0.197	0.199

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Hepatic oxidative stress, up-regulation of pro-inflammatory cytokines, apoptotic and oncogenic markers following 2-methoxyethanol administrations in rats OT Somade,Biochemistry and Biophysics Reports,2020
Ethylene glycol monomethyl ether-induced testicular oxidative stress and time-dependent up-regulation of apoptotic, pro-inflammatory, and oncogenic markers in rats OT Somade,Metabolism Open,2020
Protective effects of Artemisia judaica extract compared to metformin against hepatorenal injury in high-fat diet/streptozotocine-induced diabetic rats G Albasher,Environmental Science and Pollution Research,2020
Methyl cellosolve-induced renal oxidative stress and time-dependent up-regulation of pro-inflammatory cytokines, apoptotic, and oncogenic markers in rats OT Somade,Toxicology Reports,2020
Impact of Coenzyme Q10 Administration on Lead Acetate-Induced Testicular Damage in Rats M El-khadragy,Oxid Med Cell Longev,2020
Investigation of antioxidant and anti-inflammatory activities as well as the renal protective potential of green coffee extract in high fat-diet/streptozotocin-induced diabetes in male albino rats OD AlAmri,Journal of Functional Foods,2020
Neuroprotective role of luteolin against lead acetate-induced cortical damage in rats Baty RS,Hum Exp Toxicol,2020
Antagonistic Efficacy of Luteolin against Lead Acetate Exposure-Associated with Hepatotoxicity is Mediated via Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Activities AL-Megrin W A,Antioxidants,2019
Effect of maternal omega-3 fatty acids and vitamin E supplementation on placental apoptotic markers in rat model of early and late onset preeclampsia Vaishali Kasture, et al,Life Sciences,2019
Oleuropein suppresses oxidative, inflammatory, and apoptotic responses following glycerol-induced acute kidney injury in rats Yin M, et al,Life Sciences,2019
Nephroprotective Role of Selenium Nanoparticles Against Glycerol-Induced Acute Kidney Injury in Rats AlBasher G, et al,Biological Trace Element Research,2019
Ameliorative Effect of Beta vulgaris Root Extract on Chlorpyrifos-Induced Oxidative Stress, Inflammation and Liver Injury in Rats Albasher G, et al,Biomolecules,2019
Sevoflurane reduces ischemic brain injury in rats with diet and streptozotocin-induced diabetes Zhang H,Journal of Receptors and Signal Transduction,2019
The Cardioprotective Effects of Remote Ischemic Conditioning in a Rat Model of Acute Myocardial Infarction You L, et al,Medical science monitor,2019
Olmesartan ameliorates chemically-induced ulcerative colitis in rats via modulating NFκB and Nrf-2/HO-1 signaling crosstalk Saber S, et al,Toxicology and Applied Pharmacology,2018
Epigallocatechin-3-gallate protects against diabetic cardiomyopathy through modulating the cardiometabolic risk factors, oxidative stress, inflammation, cell death and fibrosis in streptozotocin-nicotinamide-induced diabetic rats Othman A.et al,Biomed Pharmacother.,2017
Effect of Ukrain on Ischemia/Reperfusion-Induced Kidney Injury in Rats Aras B.et al,J Surg Res.,2016
Effect of ukrain on ischemia/reperfusion-induced kidney injury in rats Bekir Aras. et al,Journal of Surgical Research,2015

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Function	Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Subcellular Location	Isoform Alpha: Mitochondrion outer membrane, Single-pass membrane protein, Cytoplasm
Protein Families	Bcl-2 family
Tissue Specificity	Expressed in a wide variety of tissues, with highest levels in the testis and ovary.
Database Links	STRING: 10116.ENSRNOP00000028328 UniGene: Rn.10668

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Related pathways

p53 signaling pathway Apoptosis Protein processing in endoplasmic reticulum Necroptosis AGE-RAGE signaling pathway in diabetic complications Neurotrophin signaling pathway

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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