Rat Apoptosis regulator BAX(BAX) ELISA kit

Rat Apoptosis regulator BAX(BAX) ELISA kit

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Rat Apoptosis regulator BAX(BAX) ELISA kit

Specification
Target

Code

CSB-EL002573RA

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Product Type

ELISA kit

Size

96T,5×96T,10×96T

Uniprot No.

Q63690

Lead Time

3-5 working days

Abbreviation

BAX

Target Name

Apoptosis regulator BAX(BAX)

Alias

BCL2L4, apoptosis regulator BAX

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat BAX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-92
1:2	Average %	96
	Range %	91-105
1:4	Average %	90
	Range %	87-99
1:8	Average %	93
	Range %	82-98

Recovery

The recovery of rat BAX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-97
EDTA plasma (n=4)	97	93-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.091	1.995	2.043	1.844
2000	1.751	1.776	1.764	1.565
1000	1.368	1.432	1.400	1.201
500	0.985	0.972	0.979	0.780
250	0.535	0.544	0.540	0.341
125	0.348	0.352	0.350	0.151
62.5	0.244	0.237	0.241	0.042
0	0.201	0.197	0.199

References

Ameliorative Effect of Beta vulgaris Root Extract on Chlorpyrifos-Induced Oxidative Stress, Inflammation and Liver Injury in Rats Albasher G, et al,Biomolecules,July 7
Effect of ukrain on ischemia/reperfusion-induced kidney injury in rats Bekir Aras. et al,Journal of Surgical Research,2015 online
Effect of Ukrain on Ischemia/Reperfusion-Induced Kidney Injury in Rats Aras B.et al,J Surg Res.,May 15
Epigallocatechin-3-gallate protects against diabetic cardiomyopathy through modulating the cardiometabolic risk factors, oxidative stress, inflammation, cell death and fibrosis in streptozotocin-nicotinamide-induced diabetic rats Othman A.et al,Biomed Pharmacother.,/
Olmesartan ameliorates chemically-induced ulcerative colitis in rats via modulating NFκB and Nrf-2/HO-1 signaling crosstalk Saber S, et al,Toxicology and Applied Pharmacology,December 27
The Cardioprotective Effects of Remote Ischemic Conditioning in a Rat Model of Acute Myocardial Infarction You L, et al,Medical science monitor,March 8
Sevoflurane reduces ischemic brain injury in rats with diet and streptozotocin-induced diabetes Zhang H,Journal of Receptors and Signal Transduction,April 30
Nephroprotective Role of Selenium Nanoparticles Against Glycerol-Induced Acute Kidney Injury in Rats AlBasher G, et al,Biological Trace Element Research,July 1
Oleuropein suppresses oxidative, inflammatory, and apoptotic responses following glycerol-induced acute kidney injury in rats Yin M, et al,Life Sciences,July 4

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Function	Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Subcellular Location	Isoform Alpha: Mitochondrion outer membrane, Single-pass membrane protein, Cytoplasm
Protein Families	Bcl-2 family
Tissue Specificity	Expressed in a wide variety of tissues, with highest levels in the testis and ovary.
Database Links	STRING: 10116.ENSRNOP00000028328 UniGene: Rn.10668
Pathway	p53 signaling pathway Apoptosis Protein processing in endoplasmic reticulum Necroptosis AGE-RAGE signaling pathway in diabetic complications Neurotrophin signaling pathway

Bax Protein

Bax Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-CF720536RA	Recombinant Rat Apoptosis regulator BAX(Bax)	in vitro E.coli expression system
CSB-YP720536RA1 CSB-EP720536RA1 CSB-BP720536RA1 CSB-MP720536RA1	Recombinant Rat Apoptosis regulator BAX(Bax) ,partial	Yeast E.coli Baculovirus Mammalian cell

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.