Rat Apoptosis regulator BAX(BAX) ELISA kit

Code CSB-EL002573RA
See More Details 24T ELISA kits trial application
Product Type ELISA kit
Size 96T,5×96T,10×96T
Uniprot No. Q63690
Lead Time 3-5 working days
Abbreviation BAX
Target Name Apoptosis regulator BAX(BAX)
Alias BCL2L4, apoptosis regulator BAX
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat BAX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %89
Range %85-92
1:2Average %96
Range %91-105
1:4Average %90
Range %87-99
1:8Average %93
Range %82-98
Recovery
The recovery of rat BAX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9288-97
EDTA plasma (n=4)9793-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.091 1.995 2.043 1.844
20001.751 1.776 1.764 1.565
10001.368 1.432 1.400 1.201
5000.985 0.972 0.979 0.780
2500.535 0.544 0.540 0.341
1250.348 0.352 0.350 0.151
62.50.244 0.237 0.241 0.042
00.201 0.197 0.199
References
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Function Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Subcellular Location Isoform Alpha: Mitochondrion outer membrane, Single-pass membrane protein, Cytoplasm
Protein Families Bcl-2 family
Tissue Specificity Expressed in a wide variety of tissues, with highest levels in the testis and ovary.
Database Links

STRING: 10116.ENSRNOP00000028328

UniGene: Rn.10668

Pathway p53 signaling pathway
Apoptosis
Protein processing in endoplasmic reticulum
Necroptosis
AGE-RAGE signaling pathway in diabetic complications
Neurotrophin signaling pathway

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