Rat Caspase 3,Casp-3 ELISA Kit

Instructions
Code CSB-E08857r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name caspase 3, apoptosis-related cysteine peptidase
Alternative Names Casp3 ELISA Kit; Cpp32Caspase-3 ELISA Kit; CASP-3 ELISA Kit; EC 3.4.22.56 ELISA Kit; Apopain ELISA Kit; Cysteine protease CPP32 ELISA Kit; CPP-32 ELISA Kit; IRP ELISA Kit; LICE ELISA Kit; Protein Yama ELISA Kit; SREBP cleavage activity 1 ELISA Kit; SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12] ELISA Kit
Abbreviation CASP3
Uniprot No. P55213
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Casp-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-92  
1:2 Average % 98  
Range % 91-105  
1:4 Average % 99  
Range % 92-110  
1:8 Average % 91  
Range % 86-98  
Recovery
The recovery of rat Casp-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 96 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.915 2.903 2.909 2.828  
10 2.321 2.283 2.302 2.221  
5 1.467 1.444 1.456 1.375  
2.5 0.727 0.706 0.717 0.636  
1.25 0.365 0.359 0.362 0.281  
0.625 0.216 0.206 0.211 0.130  
0.312 0.137 0.129 0.133 0.052  
0 0.084 0.078 0.081    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Target Data

Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity).
Gene References into Functions
  1. Authors reinforce the potential of CASP3 as an interesting target to be explored in adult and pediatric cHL, and alert for its dual biological role in H-RS cells and tumor microenvironment. PMID: 29901224
  2. Upregulation of miR-133b-5p contributes to hypoxic preconditioning -mediated cardioprotection in cardiomyocytes, and the mechanism may be associated with inhibition of caspase-8 and caspase-3 apoptotic signaling. PMID: 29568969
  3. The protein expression values of PARP and Caspase-3 in A/R cardiomyocytes were much higher than the Control. After knockdown of lncRNA AK12348, the values decreased. PMID: 29398472
  4. These results indicate that CO2 may serve as a gasotransmitter to regulate cellular function by depressing MAPK and caspase 3 activities PMID: 28555276
  5. Omi/HtrA2-HAX-1 chain played a significant role in mitochondrial homeostasis. PMID: 27998213
  6. Studied Panax notoginseng saponins regulation of signal pathway involving mTOR/Akt, caspase-3, and miR-181b in rat model of severe acute pancreatitis. PMID: 29402262
  7. the present study demonstrated that in rats with hypoxic-ischemic brain damage , there is a positive correlation between increased apoptosis of myocardial cells and expression levels of calpain-1 and caspase-3. PMID: 28447745
  8. Results provide evidence for the first time for the activation of an autophagic BECN 1 gene in the medial temporal lobe cortex during 30 days after brain ischemia. The overexpression of the BECN 1 gene was accompanied by an early (during 2 days) downregulation of both BNIP 3 and caspase 3 genes. Next, during 7-30 days expression of both genes was significantly upregulated. PMID: 27472881
  9. Bone marrow-derived mesenchymal stem cells mitigate caspase-3 and 8-hydroxy proline induced via beta-adrenergic agonist in pulmonary injured rats. PMID: 28266775
  10. The neuroprotective effect of spermidine may be related to inhibition of the caspase 3-mediated Beclin 1 cleavage and restoration of the Beclin 1-dependent autophagy. PMID: 28383560
  11. Data suggest that increase in insulin sensitivity of extensor digitorum longus muscle following low-protein, high-carbohydrate contributes to reduced proteolysis through reduction in activity of caspase-3 and ubiquitin-proteasome system. PMID: 27239756
  12. BDNF pro-peptide regulates dendritic spines via CASP3. PMID: 27310873
  13. Results suggest that active caspase-3 appears to mediate Purkinje cells (PC) death in the shaker mutant rat. Shaker mutant rat is a unique animal model of progressive PC degeneration, due to its compartmentalization and adult onset. Thus, caspase inhibitors might be used in an attempt to inhibit PC death in order to prevent the subsequent disease and/or ameliorate its symptoms. PMID: 28040459
  14. Data indicate the role of a cathepsin-caspase 3 axis in the nuclear translocation of transcription Factor EB (TFEB) leading to lysosome biogenesis under conditions of sub-lethal oxidative stress. PMID: 28002813
  15. Data show that receptor-interacting serine-threonine kinase 3 (RIPK3) played a central role in Deltamethrin (DLM)-mediated caspase 3-independent death in liver cells. PMID: 27622324
  16. In summary, our results suggest that HO-1 can decrease H/R-induced myocardiac cell apoptosis; the mechanism may be related to the activation of the Akt signaling pathway and, furthermore, to the inhibition of the JNK/c-Jun/caspase-3 signaling pathway. PMID: 27220977
  17. the expression of the genes that encode main pro- and antiapoptotic BAX and BCL-XL proteins, as well as caspase 3 (the main effector of apoptosis), in different brain structures of rats that were selected on a high aggression towards human (or its absence) was studied. PMID: 27830683
  18. Sphallerocarpus gracilis polysaccharide protected pancreatic beta-cells from alloxan damage by several possible mechanisms, including: (1) repairing free radical damage; (2) reducing the apoptosis of pancreatic beta-cells by inhibiting the activities of caspase-3 and bax, and enhancing the activity of bcl-2; (3) stimulating insulin secretion and upregulating the pancreatic and duodenal homeobox 1 gene PMID: 27645924
  19. Morin-5'-sulfonic acid sodium salt prevents changes in the expression of apoptosis related proteins caspase 3, Bax and Bcl 2 due to mercury induced oxidative stress. PMID: 27919736
  20. metabolic stress induced dysfunction of the islet beta-cell may, in part, be due to defective protein prenylation signaling pathway. PMID: 27802439
  21. Data show that selective activation of melanocortin 4 receptor (MC4R) decreased levels of TLR4, HMGB1 and caspase-3 mRNA in liver tissues, and significantly downregulated positive rate of NF-kappaB p65 in hepatocytes. PMID: 27412936
  22. Caspase-3 expression is downregulated in rat hepatic ischemia-reperfusion injury. PMID: 27320597
  23. Cytoplasmic and nuclear immunostaining for bax, caspase-3, calpain-1 and survivin proteins were found in the endometrial epithelium and stromal cells. PMID: 26818429
  24. The expressions the TLR4, NF-kappaB, HMGB1, and caspase-3 were inhibited. PMID: 26806574
  25. Hypoxia response element Bcl2 inhibition can effectively attenuate hypoxia-induced apoptosis resistance in pulmonary microvascular endothelial cells by downregulating Bcl2 expression and upregulating caspase-3 and P53 expression. PMID: 26456506
  26. proBDNF is the predominant form of BDNF expressed in the developing rat cortex, and its expression in this brain structure correlates with the levels of active caspase-3 during perinatal development PMID: 26047381
  27. The number of active caspase-3-positive neurons was significantly increased in cerebral ischemia and this was reduced markedly by exercise preconditioning. PMID: 26012958
  28. Results revealed that betaII-spectrin was degraded into major fragments of 110/85 kDa by calpain-2 activation and 108/80 kDa by caspase-3 activation. PMID: 25270371
  29. The data obtained suggest that the increase of caspase-3 enzyme activity could affect NEP expression via proteolytic degradation of its transcription factor AICD PMID: 26983277
  30. Compared with the control group, the model and treatment groups showed significantly upregulated caspase-3, Bcl-2, and Bax mRNA and protein levels in brain tissues, but remarkably downregulated Bcl-2 mRNA and protein levels PMID: 26125843
  31. Caspase-3 inhibition alleviates pulmonary injury, thereby preventing angiogenesis as well as the development of hepatopulmonary syndrome in common bile duct ligated rats. PMID: 25113058
  32. These results suggest that OGD-R-induced hyperpermeability is ROS and caspase-3 dependent and can be regulated by their inhibitors. PMID: 24372803
  33. Neonatal testosterone acts via ESR1 in an caspase dependent manner to suppress central pattern generators required for pulsatile oxytocin release. PMID: 24518793
  34. It reduced the overexpression of the angiogenic and anti-apoptotic factors TGF-beta and Akt and improved caspase-3 expression. PMID: 25519685
  35. NGF induces removal of active caspase-3 in a lysosome-dependent manner. PMID: 24787014
  36. TNF-alpha released from Kupffer cells treated with LPS may induce hepatic naofen expression, which then stimulates hepatocellular apoptosis through activation of caspase-3. PMID: 24803807
  37. From this current study, it can be concluded that administration of curcumin improved atrazine-induced cardiotoxicity through its modulatory effect on redox status, mitochondrial function, and caspase-3 expression. PMID: 24863870
  38. Treatment with 6-OHDA increased PC12 increased caspase-3 mRNA levels by about sevenfold. CDNF pretreatment attenuated this. PMID: 24633814
  39. microwave radiation induced neural cell apoptosis via the classical mitochondria-dependent caspase-3 pathway PMID: 24688304
  40. Dual vulnerability of TDP-43 to calpain and caspase-3 proteolysis after neurotoxic conditions and traumatic brain injury. PMID: 24917042
  41. Lower survival in SHR cells correlated with increased DNA fragmentation, chromatin condensation, and caspase-3 activity, indicating apoptosis. PMID: 23220262
  42. The rat model for GA I showed mitochondrial apoptotic pathway may be involved in the GA-induced striatal lesion. PMID: 22282255
  43. The novel caspase-3 substrate Gap43 is involved in AMPA receptor endocytosis and long-term depression. PMID: 24023391
  44. Myocardium Caspase-3 mRNA, SOCS-1, SOCS-3, tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) expression levels and Apoptosis index in myocardium ischemia-reperfusion rats, were examined. PMID: 24091940
  45. Inhibition of calpain activity also prevented caspase-3 activation. PMID: 23471945
  46. Manganese treatment induced robust Sp1 phosphorylation and increased its DNA binding activity. Overexpression of mutant Sp1 lacking phosphorylation sites attenuated Sp1's ability to stimulate manganese-induced caspase-3 promoter activity. PMID: 22975163
  47. Caspase-3 and GSK-3beta activation might participate in the dopaminergic cell death in a rat model of 6-hydroxydopamine induced Parkinson disease. PMID: 23940672
  48. Results suggest that caspase 3 and caspase 9-dependent signaling is necessary and sufficient to promote cardiomyocyte hypertrophy. PMID: 24101493
  49. S100a6/Casp gene expression between the prefrontal cortex and the cerebellum in spatial memory. PMID: 23792135
  50. The intensity of active caspase-3 in the granule cell layer of dentate gyrus is associated with cell proliferation and differentiation. PMID: 22959929
  51. exogenous NGAL can inhibit the activation of caspase-3, reduce the expression of Bax, and thus reduce renal tubular cell apoptosis and protect renal function in I/R injury rats. PMID: 23151253
  52. Sanhuangyinchi decoction can improve the liver function and inhibit the activity of caspase-3 in rats with acute hepatic failure. PMID: 21097400
  53. expression of cleaved caspase 3, was increased in magnocellular neurons in experimental sepsis...This in turn could compromise AVP secretion in the late phase of sepsis PMID: 23523151
  54. Caspase-3 is involved in part in apoptosis in the dentate gyrus cell layer of the hippocampus in septic rats. PMID: 23054679
  55. Administration of probiotic supernatants of E. coli Nissle 1917 and Lactobacillus rhamnosus GG decreased casp3 activity (a marker of apoptosis) in 5-fluorouracil treated intestinal epithelial cells. PMID: 22526145
  56. Data indicate tjat AT1 receptor blockade completely abrogated AT1-AA-induced TNF-alpha secretion, caspase-3 activation, and cardiomyocyte apoptosis. PMID: 23089979
  57. Transplantation of endothelial progenitor cells significantly reduced the cerebral infarct volume, decreased caspase-3 activity, upregulated Bcl-2 expression, and downregulated the expression of Bax and NF-kappaB. PMID: 23151725
  58. Lycopene counteracts the hepatic response to DMBA by altering the hepatic expression of caspase 3. PMID: 22978712
  59. relationship between caspase-3 activation and steroidogenesis and indicates that caspase-3 plays a critical role in progesterone production by granulosa cells PMID: 22963862
  60. Neuron apoptosis induces the up-regulation of GAP-43 expression leading to hyperalgesia, which can be prevented by inhibition of caspase-3. PMID: 23119112
  61. A high dose of propofol exposure may destroy the structure of neurons, induce neurodegeneration, increase Caspase-3 activity and inhibit survivin expression in hippocampus of newborn rats in vivo. PMID: 22883038
  62. Upregulation of CYP2J3/11,12-epoxyeicosatrienoic acid is involved in cardioprotection by inhibiting apoptosis via a caspase-dependent pathway. PMID: 22717287
  63. exposure to the EMF of 1950-MHz TD-SCDMA may not promote the tumor formation, but continuous exposure damaged the mitochondria of astrocytes and induce apoptosis through a caspase-3-dependent pathway with the involvement of bax and bcl-2. PMID: 22870319
  64. IL-1beta and Caspase-3 gene expressions were downregulated in rat brain damaged by cerebral ischemia/reperfusion after treatment with chuanxiongzine-astragaloside IV. PMID: 21681347
  65. N-acetylcysteine deregulated Casp3, which inhibited apoptosis and protected lungs from ischemia/reperfusion injury. PMID: 22316545
  66. In a neonatal hypoxic-ischemic rat model, hypoxia/hypoxia-ischemia treatment induces neuronal apoptosis and expression of telomerase reverse transcriptase (TERT) and cleaved caspase 3. PMID: 22446191
  67. The sciatic nerve in diabetic rats exhibit a larger number of ATF 3 expressing Schwann cells at the site of the lesion and a higher number of caspase 3 expressing Schwann cells as well. PMID: 22446192
  68. The neuroprotective effect of acute moderate alcohol consumption on caspase-3 mediated neuroapoptosis in traumatic brain injury: the role of lysosomal cathepsin L and nitric oxide. PMID: 23099040
  69. study suggests that low frequency and intensity ultrasound (LFU) can induce apoptosis in vitro and in vivo, and that three signaling proteins, caspase-3, Bcl-2, and survivin, might be involved in LFU-induced apoptosis PMID: 22819929
  70. Glutamine decreases expression of caspase-3 in both oxidant and heat-stressed intestinal epithelial cells. PMID: 22544840
  71. In terms of the mechanism of apoptosis induction by Nemo-like kinase (NLK), there is a possibility that NLK may suppress the expression of caspase-3. PMID: 22127415
  72. GABA receptor co-activation inhibits Fas/FasL apoptosis, kainic-acid-induced thioredoxin reductase activity, & procaspase-3 activation by diminishing the denitrosylation of its active-site thiol & decreasing cleavage to its active subunits. PMID: 22613773
  73. Aloe polysaccharides are able to inhibit caspase-3 expression, limit neuronal apoptosis and provide neuroprotection. PMID: 22641427
  74. Hypoxia can induce mitochondrion-dependent Caspase-3 activation in cardiomyocytes and therefore lead to cell apoptosis. PMID: 21166152
  75. Expressions of Akt, Casp3 and p38 in cardiac and somatic muscles of acute myocardial ischemia rats were higher than those in normal rats. PMID: 19548559
  76. Subacute melamine treatment could induce a significant alteration of apoptosis Bcl-2, Bax and Caspase-3 mRNA expression in rat kidney tissue. PMID: 21126425
  77. study identified JNK/c-Jun/caspase-3 as an important cascades involved in heroin-induced neurotoxicity PMID: 21856377
  78. Caspase-3 is a target gene of c-Jun:ATF2 heterodimers during apoptosis induced by activity deprivation in cerebellar granule cells. PMID: 21996423
  79. MiR-378 inhibited caspase-3 expression and attenuated ischemic injury in cardiomyocytes PMID: 22119805
  80. Yikunning can decrease the apoptotic rate and down-regulate the expression of caspase-3 mRNA and protein in rat ovaries during the perimenopausal period. PMID: 21038679
  81. Intracellular zinc depletion upregulates Caspase-3 in cultured hippocampus neurons. PMID: 22305649
  82. These data showed for the first time the role of caspase-3 in the mechanism of secondary injury of separated infarction in cerebellar cortex after middle cerebral artery occlusion in rats. PMID: 22194118
  83. Lumbar nerve root compression results in overexpression of caspase-3 in nerve root tissue and increase of dorsal root ganglion neuron apoptosis. Yiqi Huayu Recipe can inhibit the overexpression of caspase-3. PMID: 21144461
  84. Data suggest that it is possible that activation of p38/MAPK as well as reduction in Bcl2 lead to caspase activity and cell death. PMID: 21858143
  85. MicroRNA let-7e may protect PC12 cells against apoptosis following anoxia/reoxygenation injury by negatively regulating the expression of caspase 3 PMID: 21827835
  86. Iron given to rats in the neonatal period induces increased levels of caspase-3 in hippocampal regions and cortex of adult rats. PMID: 20369315
  87. Acupuncture pre-conditioning can decrease neuronal apoptosis after ischemia-reperfusion injury through a lowered expression of caspase-3 protein in senile rats. PMID: 21756776
  88. Rat prefrontal cortex was more sensitive to chronic stress; it exhibited initiation of mitochondrial apoptotic signaling via relocation of p53, cytochrome c, activation of caspase-3, and apoptotic cell death. PMID: 21656845
  89. These data shed light on the modulatory role of the VIP/PHI system on the glutamatergic transmission in amyotrophic lateral sclerosis. PMID: 21730107
  90. Caspase-3 is strongly expressed in distal renal tubular cells in rat kidney with renal tubular damage induced by lipopolysaccharide and hypoxia. PMID: 19861275
  91. Immediate nerve repair is associated with a lower amount of cleaved caspase 3 in Schwann cells. PMID: 21251262
  92. Phenotype of distinct primary sensory afferent subpopulations and caspase-3 expression following axotomy PMID: 21674244
  93. Diosmin inhibited caspase-3 activity and suppressed LPS-induced apoptosis in PC12 cells. PMID: 21477647
  94. After the administration of paraquat, the levels of PUMA, cleaved caspase-3, and APE increased in a time-dependent manner, indicating that PQ-induced nephrotoxicity was caused by activation of the apoptotic pathway. PMID: 21570442
  95. Compared with the control group, the expression of Fas mRNA and its protein and caspase-8 mRNA and the activity of caspase-8 and caspase-3 of ovary tissues in the rats treated with formaldehyde significantly increased with dose. PMID: 20448356
  96. NOS inhibition prevents retinal ganglion cell apoptosis by decreasing cleaved caspase-3 and Dexras1 expression PMID: 21245950
  97. The interaction between survivin and caspase-3 regulated the apoptosis and promoted cochlear morphological development. PMID: 19035263
  98. Regulator of calcineurin 1 (RCAN1) facilitates neuronal apoptosis through caspase-3 activation. PMID: 21216952
  99. Minocycline could inhibit expression of Caspase-3 in rats with sinusitis. PMID: 17357523
  100. The mitochondrial translocation of Bax by increased oxidative stress may result in cell death through caspase-dependent apoptosis in aged ileum, thereby leading to a decrease in intestinal mucosal thickness during aging. PMID: 20840861

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Subcellular Location Cytoplasm
Protein Families Peptidase C14A family
Tissue Specificity Expressed in heart, brain, liver, and muscle but not in kidney or testis.
Database Links

KEGG: rno:25402

STRING: 10116.ENSRNOP00000014096

UniGene: Rn.10562

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