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Rat Caspase 3,Casp-3 ELISA Kit

Citations (143) Protocol MSDS
Code CSB-E08857r
Size 96T,5×96T,10×96T
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Product Details

Target Name
caspase 3, apoptosis-related cysteine peptidase
Alternative Names
Casp3 ELISA Kit; Cpp32Caspase-3 ELISA Kit; CASP-3 ELISA Kit; EC 3.4.22.56 ELISA Kit; Apopain ELISA Kit; Cysteine protease CPP32 ELISA Kit; CPP-32 ELISA Kit; IRP ELISA Kit; LICE ELISA Kit; Protein Yama ELISA Kit; SREBP cleavage activity 1 ELISA Kit; SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12] ELISA Kit
Abbreviation
CASP3
Uniprot No.
P55213
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Casp-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-92  
1:2 Average % 98  
Range % 91-105  
1:4 Average % 99  
Range % 92-110  
1:8 Average % 91  
Range % 86-98  
Recovery
The recovery of rat Casp-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 96 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.915 2.903 2.909 2.828  
10 2.321 2.283 2.302 2.221  
5 1.467 1.444 1.456 1.375  
2.5 0.727 0.706 0.717 0.636  
1.25 0.365 0.359 0.362 0.281  
0.625 0.216 0.206 0.211 0.130  
0.312 0.137 0.129 0.133 0.052  
0 0.084 0.078 0.081 　  
Materials provided
    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Rat CASP3 antibody.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled CASP3 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle) ---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubating.
    • An instruction manual
Materials not provided
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Rat CASP3 ELISA Kit was designed for the quantitative measurement of Rat CASP3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

Citations

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Casp3 Proteins

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Target Background

Function
(From Uniprot)
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress. Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface.
Gene References into Functions
  1. Authors reinforce the potential of CASP3 as an interesting target to be explored in adult and pediatric cHL, and alert for its dual biological role in H-RS cells and tumor microenvironment. PMID: 29901224
  2. Upregulation of miR-133b-5p contributes to hypoxic preconditioning -mediated cardioprotection in cardiomyocytes, and the mechanism may be associated with inhibition of caspase-8 and caspase-3 apoptotic signaling. PMID: 29568969
  3. The protein expression values of PARP and Caspase-3 in A/R cardiomyocytes were much higher than the Control. After knockdown of lncRNA AK12348, the values decreased. PMID: 29398472
  4. These results indicate that CO2 may serve as a gasotransmitter to regulate cellular function by depressing MAPK and caspase 3 activities PMID: 28555276
  5. Omi/HtrA2-HAX-1 chain played a significant role in mitochondrial homeostasis. PMID: 27998213
  6. Studied Panax notoginseng saponins regulation of signal pathway involving mTOR/Akt, caspase-3, and miR-181b in rat model of severe acute pancreatitis. PMID: 29402262
  7. the present study demonstrated that in rats with hypoxic-ischemic brain damage , there is a positive correlation between increased apoptosis of myocardial cells and expression levels of calpain-1 and caspase-3. PMID: 28447745
  8. Results provide evidence for the first time for the activation of an autophagic BECN 1 gene in the medial temporal lobe cortex during 30 days after brain ischemia. The overexpression of the BECN 1 gene was accompanied by an early (during 2 days) downregulation of both BNIP 3 and caspase 3 genes. Next, during 7-30 days expression of both genes was significantly upregulated. PMID: 27472881
  9. Bone marrow-derived mesenchymal stem cells mitigate caspase-3 and 8-hydroxy proline induced via beta-adrenergic agonist in pulmonary injured rats. PMID: 28266775
  10. The neuroprotective effect of spermidine may be related to inhibition of the caspase 3-mediated Beclin 1 cleavage and restoration of the Beclin 1-dependent autophagy. PMID: 28383560
  11. Data suggest that increase in insulin sensitivity of extensor digitorum longus muscle following low-protein, high-carbohydrate contributes to reduced proteolysis through reduction in activity of caspase-3 and ubiquitin-proteasome system. PMID: 27239756
  12. BDNF pro-peptide regulates dendritic spines via CASP3. PMID: 27310873
  13. Results suggest that active caspase-3 appears to mediate Purkinje cells (PC) death in the shaker mutant rat. Shaker mutant rat is a unique animal model of progressive PC degeneration, due to its compartmentalization and adult onset. Thus, caspase inhibitors might be used in an attempt to inhibit PC death in order to prevent the subsequent disease and/or ameliorate its symptoms. PMID: 28040459
  14. Data indicate the role of a cathepsin-caspase 3 axis in the nuclear translocation of transcription Factor EB (TFEB) leading to lysosome biogenesis under conditions of sub-lethal oxidative stress. PMID: 28002813
  15. Data show that receptor-interacting serine-threonine kinase 3 (RIPK3) played a central role in Deltamethrin (DLM)-mediated caspase 3-independent death in liver cells. PMID: 27622324
  16. In summary, our results suggest that HO-1 can decrease H/R-induced myocardiac cell apoptosis; the mechanism may be related to the activation of the Akt signaling pathway and, furthermore, to the inhibition of the JNK/c-Jun/caspase-3 signaling pathway. PMID: 27220977
  17. the expression of the genes that encode main pro- and antiapoptotic BAX and BCL-XL proteins, as well as caspase 3 (the main effector of apoptosis), in different brain structures of rats that were selected on a high aggression towards human (or its absence) was studied. PMID: 27830683
  18. Sphallerocarpus gracilis polysaccharide protected pancreatic beta-cells from alloxan damage by several possible mechanisms, including: (1) repairing free radical damage; (2) reducing the apoptosis of pancreatic beta-cells by inhibiting the activities of caspase-3 and bax, and enhancing the activity of bcl-2; (3) stimulating insulin secretion and upregulating the pancreatic and duodenal homeobox 1 gene PMID: 27645924
  19. Morin-5'-sulfonic acid sodium salt prevents changes in the expression of apoptosis related proteins caspase 3, Bax and Bcl 2 due to mercury induced oxidative stress. PMID: 27919736
  20. metabolic stress induced dysfunction of the islet beta-cell may, in part, be due to defective protein prenylation signaling pathway. PMID: 27802439
  21. Data show that selective activation of melanocortin 4 receptor (MC4R) decreased levels of TLR4, HMGB1 and caspase-3 mRNA in liver tissues, and significantly downregulated positive rate of NF-kappaB p65 in hepatocytes. PMID: 27412936
  22. Caspase-3 expression is downregulated in rat hepatic ischemia-reperfusion injury. PMID: 27320597
  23. Cytoplasmic and nuclear immunostaining for bax, caspase-3, calpain-1 and survivin proteins were found in the endometrial epithelium and stromal cells. PMID: 26818429
  24. The expressions the TLR4, NF-kappaB, HMGB1, and caspase-3 were inhibited. PMID: 26806574
  25. Hypoxia response element Bcl2 inhibition can effectively attenuate hypoxia-induced apoptosis resistance in pulmonary microvascular endothelial cells by downregulating Bcl2 expression and upregulating caspase-3 and P53 expression. PMID: 26456506
  26. proBDNF is the predominant form of BDNF expressed in the developing rat cortex, and its expression in this brain structure correlates with the levels of active caspase-3 during perinatal development PMID: 26047381
  27. The number of active caspase-3-positive neurons was significantly increased in cerebral ischemia and this was reduced markedly by exercise preconditioning. PMID: 26012958
  28. Results revealed that betaII-spectrin was degraded into major fragments of 110/85 kDa by calpain-2 activation and 108/80 kDa by caspase-3 activation. PMID: 25270371
  29. The data obtained suggest that the increase of caspase-3 enzyme activity could affect NEP expression via proteolytic degradation of its transcription factor AICD PMID: 26983277
  30. Compared with the control group, the model and treatment groups showed significantly upregulated caspase-3, Bcl-2, and Bax mRNA and protein levels in brain tissues, but remarkably downregulated Bcl-2 mRNA and protein levels PMID: 26125843
  31. Caspase-3 inhibition alleviates pulmonary injury, thereby preventing angiogenesis as well as the development of hepatopulmonary syndrome in common bile duct ligated rats. PMID: 25113058
  32. These results suggest that OGD-R-induced hyperpermeability is ROS and caspase-3 dependent and can be regulated by their inhibitors. PMID: 24372803
  33. Neonatal testosterone acts via ESR1 in an caspase dependent manner to suppress central pattern generators required for pulsatile oxytocin release. PMID: 24518793
  34. It reduced the overexpression of the angiogenic and anti-apoptotic factors TGF-beta and Akt and improved caspase-3 expression. PMID: 25519685
  35. NGF induces removal of active caspase-3 in a lysosome-dependent manner. PMID: 24787014
  36. TNF-alpha released from Kupffer cells treated with LPS may induce hepatic naofen expression, which then stimulates hepatocellular apoptosis through activation of caspase-3. PMID: 24803807
  37. From this current study, it can be concluded that administration of curcumin improved atrazine-induced cardiotoxicity through its modulatory effect on redox status, mitochondrial function, and caspase-3 expression. PMID: 24863870
  38. Treatment with 6-OHDA increased PC12 increased caspase-3 mRNA levels by about sevenfold. CDNF pretreatment attenuated this. PMID: 24633814
  39. microwave radiation induced neural cell apoptosis via the classical mitochondria-dependent caspase-3 pathway PMID: 24688304
  40. Dual vulnerability of TDP-43 to calpain and caspase-3 proteolysis after neurotoxic conditions and traumatic brain injury. PMID: 24917042
  41. Lower survival in SHR cells correlated with increased DNA fragmentation, chromatin condensation, and caspase-3 activity, indicating apoptosis. PMID: 23220262
  42. The rat model for GA I showed mitochondrial apoptotic pathway may be involved in the GA-induced striatal lesion. PMID: 22282255
  43. The novel caspase-3 substrate Gap43 is involved in AMPA receptor endocytosis and long-term depression. PMID: 24023391
  44. Myocardium Caspase-3 mRNA, SOCS-1, SOCS-3, tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) expression levels and Apoptosis index in myocardium ischemia-reperfusion rats, were examined. PMID: 24091940
  45. Inhibition of calpain activity also prevented caspase-3 activation. PMID: 23471945
  46. Manganese treatment induced robust Sp1 phosphorylation and increased its DNA binding activity. Overexpression of mutant Sp1 lacking phosphorylation sites attenuated Sp1's ability to stimulate manganese-induced caspase-3 promoter activity. PMID: 22975163
  47. Caspase-3 and GSK-3beta activation might participate in the dopaminergic cell death in a rat model of 6-hydroxydopamine induced Parkinson disease. PMID: 23940672
  48. Results suggest that caspase 3 and caspase 9-dependent signaling is necessary and sufficient to promote cardiomyocyte hypertrophy. PMID: 24101493
  49. S100a6/Casp gene expression between the prefrontal cortex and the cerebellum in spatial memory. PMID: 23792135
  50. The intensity of active caspase-3 in the granule cell layer of dentate gyrus is associated with cell proliferation and differentiation. PMID: 22959929

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Subcellular Location
Cytoplasm.
Protein Families
Peptidase C14A family
Tissue Specificity
Expressed in heart, brain, liver, and muscle but not in kidney or testis.
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