Product Details

Target Name

gremlin 1, cysteine knot superfamily, homolog (Xenopus laevis)

Alternative Names

Grem1 ELISA Kit; Cktsf1b1 ELISA Kit; Drm ELISA Kit; Gremlin-1 ELISA Kit; Cysteine knot superfamily 1 ELISA Kit; BMP antagonist 1 ELISA Kit; Down-regulated in Mos-transformed cells protein ELISA Kit

Abbreviation

GREM1

Uniprot No.

O35793

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

1.56 ng/mL-100 ng/mL

Sensitivity

0.39 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat gremlin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	105
	Range %	101-109
1:2	Average %	96
	Range %	94-98
1:4	Average %	89
	Range %	83-95
1:8	Average %	93
	Range %	89-97

Recovery

The recovery of rat gremlin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	99-104
EDTA plasma (n=4)	90	85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.210	2.222	2.216	2.094
50	1.798	1.844	1.821	1.699
25	1.432	1.503	1.468	1.346
12.5	1.053	1.062	1.058	0.936
6.25	0.751	0.722	0.737	0.615
3.12	0.391	0.411	0.401	0.279
1.56	0.258	0.266	0.262	0.140
0	0.121	0.123	0.122

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Rat GREM1 ELISA Kit was designed for the quantitative measurement of Rat GREM1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

Related Products

Target Background

Function
(From Uniprot)

Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Antagonist of BMP2; inhibits BMP2-mediated differentiation of osteoblasts (in vitro). Acts as inhibitor of monocyte chemotaxis. Can inhibit the growth or viability of normal cells but not transformed cells when is overexpressed.

Gene References into Functions

gremlin1 promotes hepatic stellate cell activation and hepatic fibrogenesis through impairment of the balance between transforming growth factor beta and bone morphogenetic protein 7 signaling pathways. PMID: 27863390
The therapeutic mechanism of Danshao Huaxian capsule in hepatic fibrosis may be associated with inhibition of the expression of Gremlin and up-regulation of the expression of BMP-7. PMID: 25356047
GREM1 affects follicle transition in cultured ovaries, but is not normally present in primordial and early-stage developing follicles. PMID: 24614542
Notch pathway gene expression is elevated in diabetic nephropathy, co-incident with Gremlin, and may contribute to the pathogenesis of this dise PMID: 17980714
Gremlin plays a part in the development of pathological phenotypic changes of adult vascular smooth muscle cell. PMID: 18086474
The bone morphogenetic protein antagonist gremlin promotes vascular smooth muscle cell apoptosis. PMID: 19142012

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Subcellular Location

Secreted.

Protein Families

DAN family

Tissue Specificity

Highly expressed in the brain, kidney, spleen, and testis and weakly expressed in the lung and liver. Predominantly expressed in differentiated cells as neurons in brain, type I cells in lung and globlet cells in intestine.

Database Links

KEGG: rno:50566

STRING: 10116.ENSRNOP00000035888

UniGene: Rn.42929

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Code	CSB-E17688r
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Rat Gremlin ELISA Kit

Product Details

Citations

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Target Background