Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Grem1

Uniprot No.

O35793

Research Area

Cardiovascular

Alternative Names

Grem1; Cktsf1b1; Drm; Gremlin-1; Cysteine knot superfamily 1; BMP antagonist 1; Down-regulated in Mos-transformed cells protein

Species

Rattus norvegicus (Rat)

Source

Baculovirus

Expression Region

25-184aa

Target Protein Sequence

KKKGSQGAIPPPDKAQHNDSEQTQSPPQPGSRTRGRGQGRGTAMPGEEVLESSQEALHVTERKYLKRDWCKTQPLKQTIHEEGCNSRTIINRFCYGQCNSFYIPRHIRKEEGSFQSCSFCKPKKFTTMMVTLNCPELQPPTKKKRVTRVKQCRCISIDLD
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

22.2 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Our Recombinant Rat Gremlin-1 is a superior product for your cardiovascular research needs. This protein, also known as Cysteine knot superfamily 1, BMP antagonist 1, and Down-regulated in Mos-transformed cells protein, is encoded by the 'Grem1' gene in the Rat species. Its key role in vascular biology and organogenesis makes it an excellent tool for exploring various biological processes.

Our product, expressed in a Baculovirus system, provides the full length of the mature protein, ranging from amino acid 25 to 184. The protein is augmented with N-terminal 10xHis and C-terminal Myc tags, facilitating efficient purification and detection procedures. Ensuring optimal product quality, we maintain a purity level greater than 85% as determined by SDS-PAGE. It is available in both liquid and lyophilized powder forms, enabling you to choose the best fit for your experimental setup. Our Recombinant Rat Grem1 is designed to offer reliable performance and accuracy for your research pursuits.

Target Background

Function

Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Antagonist of BMP2; inhibits BMP2-mediated differentiation of osteoblasts (in vitro). Acts as inhibitor of monocyte chemotaxis. Can inhibit the growth or viability of normal cells but not transformed cells when is overexpressed.

Gene References into Functions

gremlin1 promotes hepatic stellate cell activation and hepatic fibrogenesis through impairment of the balance between transforming growth factor beta and bone morphogenetic protein 7 signaling pathways. PMID: 27863390
The therapeutic mechanism of Danshao Huaxian capsule in hepatic fibrosis may be associated with inhibition of the expression of Gremlin and up-regulation of the expression of BMP-7. PMID: 25356047
GREM1 affects follicle transition in cultured ovaries, but is not normally present in primordial and early-stage developing follicles. PMID: 24614542
Notch pathway gene expression is elevated in diabetic nephropathy, co-incident with Gremlin, and may contribute to the pathogenesis of this dise PMID: 17980714
Gremlin plays a part in the development of pathological phenotypic changes of adult vascular smooth muscle cell. PMID: 18086474
The bone morphogenetic protein antagonist gremlin promotes vascular smooth muscle cell apoptosis. PMID: 19142012

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Subcellular Location

Secreted.

Protein Families

DAN family

Tissue Specificity

Highly expressed in the brain, kidney, spleen, and testis and weakly expressed in the lung and liver. Predominantly expressed in differentiated cells as neurons in brain, type I cells in lung and globlet cells in intestine.

Database Links

KEGG: rno:50566

STRING: 10116.ENSRNOP00000035888

UniGene: Rn.42929

Code	CSB-BP009892RA
Abbreviation	Recombinant Rat Grem1 protein
MSDS	MSDS Datasheet
Size	$528
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Rat Gremlin-1 (Grem1)

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