Purity
Greater than 85% as determined by SDS-PAGE.
Research Area
Cardiovascular
Alternative Names
Grem1; Cktsf1b1; Drm; Gremlin-1; Cysteine knot superfamily 1; BMP antagonist 1; Down-regulated in Mos-transformed cells protein
Species
Rattus norvegicus (Rat)
Expression Region
25-184aa
Target Protein Sequence
KKKGSQGAIPPPDKAQHNDSEQTQSPPQPGSRTRGRGQGRGTAMPGEEVLESSQEALHVTERKYLKRDWCKTQPLKQTIHEEGCNSRTIINRFCYGQCNSFYIPRHIRKEEGSFQSCSFCKPKKFTTMMVTLNCPELQPPTKKKRVTRVKQCRCISIDLD
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Our Recombinant Rat Gremlin-1 is a superior product for your cardiovascular research needs. This protein, also known as Cysteine knot superfamily 1, BMP antagonist 1, and Down-regulated in Mos-transformed cells protein, is encoded by the 'Grem1' gene in the Rat species. Its key role in vascular biology and organogenesis makes it an excellent tool for exploring various biological processes.
Our product, expressed in a Baculovirus system, provides the full length of the mature protein, ranging from amino acid 25 to 184. The protein is augmented with N-terminal 10xHis and C-terminal Myc tags, facilitating efficient purification and detection procedures. Ensuring optimal product quality, we maintain a purity level greater than 85% as determined by SDS-PAGE. It is available in both liquid and lyophilized powder forms, enabling you to choose the best fit for your experimental setup. Our Recombinant Rat Grem1 is designed to offer reliable performance and accuracy for your research pursuits.