Rat Macrophage Colony-Stimulating Factor,M-CSF ELISA kit

Instructions
Code CSB-E07424r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name colony stimulating factor 1 (macrophage)
Alternative Names Csf1 ELISA kit; CsfmMacrophage colony-stimulating factor 1 ELISA kit; CSF-1 ELISA kit; MCSF) [Cleaved into: Processed macrophage colony-stimulating factor 1] ELISA kit
Abbreviation CSF1
Uniprot No. Q8JZQ0
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat M-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %82-95
1:2Average %98
Range %90-105
1:4Average %95
Range %90-100
1:8Average %90
Range %85-95
Recovery
The recovery of rat M-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9892-104
EDTA plasma (n=4)9287-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.413 2.352 2.383 2.192
5002.032 1.976 2.004 1.813
2501.658 1.543 1.601 1.410
1251.225 1.202 1.214 1.023
62.50.869 0.824 0.847 0.656
31.20.431 0.407 0.419 0.228
15.60.341 0.315 0.328 0.137
00.194 0.188 0.191  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance (By similarity).
Involvement in disease A defect in Csf1 is the cause of the toothless phenotype (tl). Toothless rats display osteoclastopenia, severe osteopetrosis, and dystrophic growth plates.
Subcellular Location Cell membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Processed macrophage colony-stimulating factor 1: Secreted, extracellular space
Database Links

STRING: 10116.ENSRNOP00000025221

UniGene: Rn.83632

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