Rat Transforming Growth factor β2,TGF-β2 ELISA kit

Code CSB-E12111r
Size 96T,5×96T,10×96T
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Product Details

Target Name
transforming growth factor, beta 2
Alternative Names
Tgfb2 ELISA Kit; Transforming growth factor beta-2 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-2 ELISA Kit; TGF-beta-2)] ELISA Kit
Abbreviation
TGFB2
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TGF-β2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %85
Range %81-89
1:2Average %95
Range %91-99
1:4Average %87
Range %82-90
1:8Average %95
Range %91-99
Recovery
The recovery of rat TGF-β2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8381-85
EDTA plasma (n=4)9691-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.869 2.897 2.883 2.691
5002.231 2.267 2.249 2.057
2501.544 1.598 1.571 1.379
1250.969 0.987 0.978 0.786
62.50.638 0.609 0.624 0.432
31.20.452 0.472 0.462 0.270
15.60.307 0.321 0.314 0.122
00.191 0.193 0.192
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The Rat Transforming Growth factor β2 (TGF-β2) ELISA kit is a high-quality tool for quantitative measurement of TGF-β2 levels in rat serum, plasma, and tissue homogenates.

The kit is designed to detect Transforming growth factor beta-2 proprotein, encoded by the Tgfb2 gene in Rattus norvegicus (Rat), which plays a key role in signal transduction pathways,cell growth, cell differentiation, apoptosis and cellular homeostasis.

With a wide detection range of 15.6 pg/mL-1000 pg/mL and a sensitivity of 3.9 pg/mL, this sandwich ELISA kit ensures accurate and precise measurements of TGF-β2 levels, even at low concentrations. The assay time is quick, ranging from 1-5 hours, with a sample volume of only 50-100ul.

The Rat Transforming Growth factor β2 (TGF-β2) ELISA kit is ideal for researchers studying signal transduction in the context of various diseases and conditions. The kit is simple to use, and the results are easily readable at a detection wavelength of 450 nm.

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Target Background

Function
(From Uniprot)
Transforming growth factor beta-2 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively.; Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2.; Transforming growth factor beta-2: Multifunctional protein that regulates various processes such as angiogenesis and heart development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus. Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.
Gene References into Functions
  1. VEGF and TGFB2 are upregulated in femoral bone during fracture healing following implantation with coated titanium screws. PMID: 28361415
  2. Diminished collagen synthesis in folliculostellate cell-deficient cell aggregates was partially recovered by TGFbeta2. TGFbeta2-mediated folliculostellate cell-pericyte interaction appears to be essential for collagen synthesis in anterior pituitary. PMID: 26957638
  3. miR-378a expression is associated with its methylation status in TGF-beta1-treated cells, and epigenetically-regulated miR-378a inhibits TGF-beta1-induced hepatic stellate cells activation, at least in part, via TGF-beta2. PMID: 27855367
  4. Hepatic TGFB2 gene expression was up-regulated in liver of bile duct ligation model of liver fibrosis. PMID: 27049496
  5. These study results suggest that HR controls Tgf-b2 expression via regulation of miR-31, thus causing abnormal hair cycle in Hr(Hp)/Hr(Hp) mice. PMID: 25639665
  6. Data suggest that Muller cells release TGF-beta2, inhibiting the proliferation of retinal endothelial cells via activation of Smad2/Smad3 and attenuation of ERK signaling PMID: 24838936
  7. level of TGFbeta2 in rat colonic neoplasms PMID: 12209600
  8. Tgfb2 exhibited dynamic changes in expression in experimental glomerulonephritis. PMID: 12545247
  9. colocalizes with the trans-Golgi network marker TGN38 and a marker molecule for secretory granules, chromogranin B in PC12 cells and hippocampal neurons; a substantial portion of TGF-beta2 is secreted by the regulated secretory pathway in these cells PMID: 12595240
  10. TGF-beta 2 may play a physiological role in germ cell proliferation/differentiation and Sertoli cell function. PMID: 12646048
  11. role in cranial suture fusion PMID: 12733949
  12. Tgfb2 exrpession increased in infarcted myocardium after propranolol treatment. PMID: 14575314
  13. Analysis of Tgf-beta2 expression patterns in acute and progressive glomerulonephritis models. PMID: 15122060
  14. TGF-beta2 mRNA and protein expressions in the cerebral cortex of the H-Tx rat, a model of congenital hydrocephalus. PMID: 15375625
  15. TGF-beta2 plays important role in pancreatic morphogenesis and administration of excess all-trans retinoic acid before neurulation inhibit TGF-beta2 expression disrupted pancreatic morphogenesis particularly Langerhans islets. PMID: 16479313
  16. differential processing of TGF-beta1 and -beta 2 may be part of the mechanism that generates isoform-specific functions of the TGF-betas PMID: 17516499
  17. expression and signaling of TGFbeta2 and distribution of extracellular matrix proteins: laminin, fibronectin, collagens in the parotid gland of control and diabetic rats. PMID: 17574405
  18. novel role for TGF-beta2 in protection from unloading-induced apoptosis in vivo PMID: 18378961
  19. TGF-beta1 and TGF-beta 2 mRNA, but not TGF-beta3, is increased at 4 and 12 weeks in experimental diabetic peripheral neuropathy. PMID: 18406405
  20. immunolocalization of transforming growth factor beta (TGF-beta2) in rat thymic stromal cells and thymocytes: TGF-beta2 may play an important role in the organization or development of thymocytes in the late stages of thymopoiesis PMID: 18554691
  21. These results show that elevated expression of TSP-1 and TGF-beta can be detected in the injured segment of the spinal cord 12 and 24 h after injury. Thus, TSP-1 and TGF-beta may have a role in SCI. PMID: 19342245
  22. The renoprotective effect of AM might be related to inhibition of myofibroblast activation, inducing of HGF and reducing of TGF-beta1 expression. PMID: 19434765
  23. TGF-beta2 is an essential neuroprotective factor released by microglia in its culture medium that, to be fully effective, requires the concomitant presence of other factor(s) of low molecular weight. PMID: 19457129
  24. TGF-beta2 and TGF-beta3 induced a dose-dependant increase of apoptosis in cultured stromal cells. PMID: 19656380

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Subcellular Location
[Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; [Transforming growth factor beta-2]: Secreted.
Protein Families
TGF-beta family
Tissue Specificity
[Isoform TGF-beta2B]: Expressed in the aorta, primary bronchus, uterus, heart, skeletal muscle, sciatic nerve and spinal cord but not in the intestine.
Database Links
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