Rat dipeptidyl peptldase Ⅳ,DPPⅣ ELISA Kit

Code CSB-E08519r
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P14740
Lead Time 3-5 working days
Abbreviation DPP4
Protein Biological Process 1 Cell Adhesion
Target Name dipeptidyl-peptidase 4
Alias ADABP, ADCP2, CD26, DPPIV, TP103, T-cell activation antigen CD26|adenosine deaminase complexing protein 2|dipeptidylpeptidase 4|dipeptidylpeptidase IV|dipeptidylpeptidase IV (CD26, adenosine deamina
Species Rattus norvegicus (Rat)
Protein Biological Process 3 Cell adhesion
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 ng/mL-1000 ng/mL
Sensitivity 3.9 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat DPPⅣin various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 94
Range % 91-97
1:2 Average % 86
Range % 83-90
1:4 Average % 101
Range % 97-105
1:8 Average % 88
Range % 85-93
Recovery
The recovery of rat DPPⅣ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 106 103-109
EDTA plasma (n=4) 95 91-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
1000 2.047 2.102 2.075 1.923
500 1.513 1.500 1.507 1.355
250 0.869 0.901 0.885 0.733
125 0.534 0.541 0.538 0.386
62.5 0.311 0.321 0.316 0.164
31.2 0.252 0.261 0.257 0.105
15.6 0.192 0.194 0.193 0.041
0 0.153 0.150 0.152  
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Function Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.
Subcellular Location Dipeptidyl peptidase 4 soluble form: Secreted, Note=Detected in the serum and the seminal fluid, SUBCELLULAR LOCATION: Cell membrane, Single-pass type II membrane protein, Apical cell membrane, Single-pass type II membrane protein, Cell projection, invadopodium membrane, Single-pass type II membrane protein, Cell projection, lamellipodium membrane, Single-pass type II membrane protein, Cell junction, Membrane raft
Protein Families Peptidase S9B family, DPPIV subfamily
Tissue Specificity Expressed in bile ducts and other epithelial brush borders (small intestine, kidney, colon, pancreatic duct); acinar structures in salivary glands; endothelial structures and T cell areas in thymus, spleen and lymph node.
Database Links

KEGG: rno:25253

STRING: 10116.ENSRNOP00000045536

UniGene: Rn.91364

Pathway Myocardial Fatty Acid Metabolism

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