Recombinant Rat Dipeptidyl peptidase 4(Dpp4),partial

Code CSB-EP007139RA
Size US$2466
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Dpp4
Uniprot No. P14740
Research Area Immunology
Alternative Names Dpp4; Cd26Dipeptidyl peptidase 4; EC 3.4.14.5; Bile canaliculus domain-specific membrane glycoprotein; Dipeptidyl peptidase IV; DPP IV; GP110 glycoprotein; T-cell activation antigen CD26; CD antigen CD26) [Cleaved into: Dipeptidyl peptidase 4 membrane form; Dipeptidyl peptidase IV membrane form); Dipeptidyl peptidase 4 soluble form; Dipeptidyl peptidase IV soluble form); Dipeptidyl peptidase 4 60 kDa soluble form; Dipeptidyl peptidase IV 60 kDa soluble form)]
Species Rattus norvegicus (Rat)
Source E.coli
Expression Region 638-767aa
Target Protein Sequence SMVLGSGSGVFKCGIAVAPVSRWEYYDSVYTERYMGLPTPEDNLDHYRNSTVMSRAENFKQVEYLLIHGTADDNVHFQQSAQISKALVDAGVDFQAMWYTDEDHGIASSTAHQHIYSHMSHFLQQCFSLR
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 30.7kDa
Protein Length Partial
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.
Gene References into Functions
  1. Study demonstrates that gestational glucocorticoid (GC) exposure during late pregnancy induced impaired glucose homeostasis that is accompanied by increased endoglin levels, atherogenic dyslipidemia and pancreatic beta cell dysfunction, increased DPP-4 activity and altered foetal outcome. Therefore, GC-induced glucose deregulation and inflammation during late gestation is through endoglin-/DPP-4-dependent pathway. PMID: 29677638
  2. Proteome profile array screening further revealed that DPPIV decreases matrix metalloproteinase-9, a key downstream effector of ERK-AKT signaling pathways PMID: 27525674
  3. results suggest that long-term exposure to DPPIV inhibitors looks compatible with an overall balanced metabolism PMID: 28895067
  4. Bis-Pyrano Prenyl Isoflavone Improves Glucose Homeostasis by Inhibiting Dipeptidyl Peptidase-4 in Hyperglycemic Rats PMID: 27238050
  5. The study demonstrates that the DPP4 inhibitor has a neuroprotective effect against hyperthermia-induced seizures by reducing the astrocyte-mediated inflammatory response by an NF-kappaB-dependent pathway. These properties are of significant clinical value and help explain the increasing evidence that DPP4 is involved in the incidence and progression of febrile seizures. PMID: 28372289
  6. DPP-4 inhibition with linagliptin may represent a novel treatment for chronic kidney disease. PMID: 27083282
  7. DPP4 inhibition protects against ventricular arrhythmias by attenuating NGF-induced sympathetic innervation via cAMP/PKA/CREB-dependent antioxidant pathway in non-diabetic infarcted rats. PMID: 26399925
  8. Dipeptidyl peptidase-4 inhibition by gemigliptin exerts a preventative effect on the proliferation and migration of VSMCs via Nrf2. PMID: 26187356
  9. Differences in Expression of DPP4 in Steatotic Rat Liver Are Not Related to Differences in the Methylation of its Gene Promoter PMID: 26359413
  10. The inhibition of the renal DPP-4 activity induced by saxagliptin may contribute to ameliorating renal injury in hypertension-related renal injury. PMID: 25936515
  11. Inhibition of DPP-4 reduces T2DM-induced increase in post-MI acute mortality possibly by restoring the autophagic response through attenuation of Bcl-2-Beclin-1 interaction. PMID: 26259714
  12. DPP4 inhibitor vildagliptin attenuates oxidative stress and cardiac fibrosis and improving cardiac function in rats with chronic myocardial infarction. PMID: 25823534
  13. Findings indicate a positive correlation between reduced stress-responsiveness and increased central NPY, in DPP4mut rats. PMID: 25635612
  14. DPP-4 inhibitors reduced macrophage infiltration via glucagon-like peptide-1-dependent signaling in nephritis model and suggest that the control of inflammation by DPP-4 inhibitors is useful for the treatment of nondiabetic kidney disease models. PMID: 25656369
  15. bone marrow derived cells but not the kidney represent at least one source of sDPP4. And leukocyte or macrophage subpopulations could be potential candidates. PMID: 24874705
  16. DPP4 regulates the expression of the hemoglobin genes, and might play a role in the preservation of renal function. PMID: 25122001
  17. Maternal deprivation as a model for postnatal stress experience influences remarkably postnatal lung development of rats, which is significantly modulated by the DPP4-system. PMID: 24357522
  18. Long term deficiency of DPP4 activity improved cardiac performance against pressure overload in rat. PMID: 24416433
  19. a low level of DPPIV activity contributed to maintaining intestinal homeostasis PMID: 23832365
  20. Circulating DPPIV activity is associated with poorer cardiovascular outcomes in heart failure. PMID: 23894014
  21. High fat and high cholesterol diets increased DPP-IV expression in intestinal lymph. PMID: 23535306
  22. DPP-IV is a promising in situ marker of biliary functionality not only of normal but also of fatty rats. PMID: 23361237
  23. Long term loss of DPP4 activity increased the capability against ROS stress, which was more than GLP-1 dependent pathway PMID: 23359639
  24. DPP-4 deficiency restored ischemia (but not G-CSF)-induced stem cell mobilization and improved vascular recovery in diabetic animals PMID: 23184393
  25. These data suggest that GLP-1 analogs may serve as a novel therapeutic drug to alleviate obesity-induced liver injury by reducing bile acid synthesis and improving liver bile secretory function. PMID: 22918684
  26. Dipeptidyl peptidase IV regulates proliferation of preglomerular vascular smooth muscle and mesangial cells. PMID: 22802229
  27. novel evidence shows the regulatory roles of DPP4 in chronic heart failure. PMID: 23035207
  28. Seven days post artery occlusion, dipeptidyl peptidase IV immunoreactivity was found in the perikarya of surviving cortical neurons of the ipsilateral hemisphere. PMID: 22373413
  29. APN and DPPIV activity levels are related to the development of arthritis, being protective or inducer of the susceptibility PMID: 21982785
  30. This study demonstrates a reduction of anabolic effects on the energy homeostasis and a centrally enhanced NPY-expression in DPP4-deficient rats under high caloric food intake. DPP4-deficiency altered intracorporal fat distribution. PMID: 20887754
  31. plasma DPP4 activity changes in accordance with the progression of hyperinsulinemic obesity and pancreatic islet atrophy PMID: 21139073
  32. Monosodium glutamate and/or food deprivation decreased the activity of dipeptidyl peptidase 4 in the soluble and membrane fraction from the hypothalamus, and in the membrane fraction from the hippocampus. PMID: 20153005
  33. Reduced airway inflammation in CD26-deficient F344 rats appear to be mediated by differences in the recruitment and activity of Tregs. PMID: 20560982
  34. APM and DPPIV-DI are respectively related to the downregulation of somatostatin in food-deprived rats, and to the recovery of energy balance in monosodium glutamate obese rats during food deprivation PMID: 19876009
  35. These findings demonstrate a negative regulatory role of the bronchus-associated lymphatic tissue -specific expression of CD26 in T-cell adhesion during an asthma-like inflammation. PMID: 19501934
  36. The 6A3 epitope was stably exposed in both native and denatured rDPP IV. Data mapped the 6A3 epitope to a surface-exposed Thr331-dependent motif D329KTTLVWN, only 11 amino acids away from L311QWLRRI on the same plane as the fifth beta-propeller blade. PMID: 19804410
  37. Immunoreactive endomorphin 2 was generated extracellularly by a membrane-bound DPP-IV, which was switched to "synthase" mode by the hydrolase inhibitor Ile-Pro-Ile PMID: 19540879
  38. Clustered charged amino acids of human adenosine deaminase comprise a functional epitope for binding the adenosine deaminase complexing protein CD26/dipeptidyl peptidase IV. PMID: 11901152
  39. DPPIV deficiency results in improved glucose tolerance and ameliorated insulin resistance PMID: 12031691
  40. kinetics of this enzyme and insights into its dimerization and gelatinase activity PMID: 12675219
  41. An increase in this enzyme is associated with graft rejection. Inhibition of this enzyme prevents heart graft rejection. PMID: 12675233
  42. The three-dimensional structure of DPPIV as determined by cryo-TEM and single particle analysis. PMID: 12705886
  43. identification of DPPIV-binding sites in fibronectin and the effect of binding site peptides on DPPIV/poly-fibronectin adhesion and metastasis PMID: 12716896
  44. The behavioral repertoire and response to ethanol was characterized in dipeptidyl peptidase IV (DPPIV/CD26)-deficient, mutant Japanese, German, and wild-type-like F344 rat substrains. PMID: 14568317
  45. presence of a yet to be characterized signalling mechanism whereby DPP IV has access to c-Src-containing signalling platforms PMID: 16478473
  46. short-term DP IV inhibition does not eliminate the satiety actions of exogenously administered peptide YY at the doses tested. PMID: 16802131
  47. results extend earlier findings and illustrate the role of CD26/DPP IV in alloantigen-mediated immune responses PMID: 16962474
  48. The X-ray structure confirms that the binding mode of rat DPPIV is similar to the parent xanthines. PMID: 17010607
  49. Inhibition of intragraft DPP IV activity significantly reduced ischemia/reperfusion-associated pulmonary injury, allowing for successful transplantation after 18 hours of ischemia. PMID: 17175274
  50. Rats with a high fat diet significantly increased DPP-IV's expression and activity about 142-152% in the intestine and 153-240% in kidneys, but there was no change in the liver. PMID: 17583752
  51. In genetically susceptible animals, inhibition of DPP4 increases arterial blood pressure via Y(1) receptors when elevated blood pressure is reduced with antihypertensives. PMID: 18047624
  52. Inhibition of DPPIV catalytic activity is associated with inhibition of NHE3-mediated NaHCO3 reabsorption in the renal proximal tubule. A physiologically significant interaction exists between NHE3 and DPPIV in the intact proximal tubule in vivo. PMID: 18077600
  53. Data suggest that alogliptin is a potent and highly selective DPP-4 inhibitor with demonstrated efficacy in Zucker fa/fa rats and potential for once-daily dosing in humans. PMID: 18538760
  54. DPP4-deficient rats were resistant to developing diabetes, while susceptible to dyslipidaemia and reduction of glomerular filtration rate by streptozotocin. PMID: 18931022
  55. Effects on basal collagen metabolism following DPP4 inhibition in vivo are demonstrated and the amyloid peptide is identified as a novel DPP4 substrate. PMID: 18940185
  56. Restoration of DPPIV expression in NB cells led to their differentiation in association with increased expression of the neural marker MAP2 and decreased expression of chemokines, including stromal-derived factor 1 (SDF1) and its receptor CXCR4. PMID: 18978811
  57. DP4 deficient rats exhibited reduced diet-induced body weight gain and improved glucose tolerance associated with increased levels of glucagon-like peptide-1 (GLP-1) and bound leptin as well as decreased aminotransferases and triglycerides PMID: 19327106

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Subcellular Location Dipeptidyl peptidase 4 soluble form: Secreted, Note=Detected in the serum and the seminal fluid, SUBCELLULAR LOCATION: Cell membrane, Single-pass type II membrane protein, Apical cell membrane, Single-pass type II membrane protein, Cell projection, invadopodium membrane, Single-pass type II membrane protein, Cell projection, lamellipodium membrane, Single-pass type II membrane protein, Cell junction, Membrane raft
Protein Families Peptidase S9B family, DPPIV subfamily
Tissue Specificity Expressed in bile ducts and other epithelial brush borders (small intestine, kidney, colon, pancreatic duct); acinar structures in salivary glands; endothelial structures and T cell areas in thymus, spleen and lymph node.
Database Links

KEGG: rno:25253

STRING: 10116.ENSRNOP00000045536

UniGene: Rn.91364

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