Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Dpp4

Uniprot No.

P14740

Research Area

Immunology

Alternative Names

Dpp4; Cd26; Dipeptidyl peptidase 4; Bile canaliculus domain-specific membrane glycoprotein; Dipeptidyl peptidase IV; DPP IV; GP110 glycoprotein; T-cell activation antigen CD26; CD antigen CD26

Species

Rattus norvegicus (Rat)

Source

E.coli

Expression Region

638-767aa

Target Protein Sequence

SMVLGSGSGVFKCGIAVAPVSRWEYYDSVYTERYMGLPTPEDNLDHYRNSTVMSRAENFKQVEYLLIHGTADDNVHFQQSAQISKALVDAGVDFQAMWYTDEDHGIASSTAHQHIYSHMSHFLQQCFSLR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

30.7kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Just like other recombinant proteins, the production of this recombinant Rat Dpp4 protein began with appropriate cDNA and PCR methods, and then the Dpp4 expression plasmids were built. Following sequence determination of the constructs, plasmids were transformed into E.coli for the expression of the recombinant Rat Dpp4 protein. N-terminal 6xHis-SUMO tag was used in the process. And we finally get the protein of interest with purity of 90%+.

Dpp4 is a gene providing an instruction of making a protein named dipeptidyl peptidase 4 in rattus norvegicus (rat). Dipeptidyl peptidase 4 is also known as bile canaliculus domain-specific membrane glycoprotein, dipeptidyl peptidase IV (DPP IV), GP110 glycoprotein and T-cell activation antigen CD26 (CD26). This protein can be cleaved into 3 chains, including dipeptidyl peptidase 4 membrane form, dipeptidyl peptidase 4 soluble form and dipeptidyl peptidase 4 60 kDa soluble form. It is involved in many biological processes, such as cell adhesion, endothelial cell migration, T cell activation, etc.

Target Background

Function

Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.

Gene References into Functions

Study demonstrates that gestational glucocorticoid (GC) exposure during late pregnancy induced impaired glucose homeostasis that is accompanied by increased endoglin levels, atherogenic dyslipidemia and pancreatic beta cell dysfunction, increased DPP-4 activity and altered foetal outcome. Therefore, GC-induced glucose deregulation and inflammation during late gestation is through endoglin-/DPP-4-dependent pathway. PMID: 29677638
Proteome profile array screening further revealed that DPPIV decreases matrix metalloproteinase-9, a key downstream effector of ERK-AKT signaling pathways PMID: 27525674
results suggest that long-term exposure to DPPIV inhibitors looks compatible with an overall balanced metabolism PMID: 28895067
Bis-Pyrano Prenyl Isoflavone Improves Glucose Homeostasis by Inhibiting Dipeptidyl Peptidase-4 in Hyperglycemic Rats PMID: 27238050
The study demonstrates that the DPP4 inhibitor has a neuroprotective effect against hyperthermia-induced seizures by reducing the astrocyte-mediated inflammatory response by an NF-kappaB-dependent pathway. These properties are of significant clinical value and help explain the increasing evidence that DPP4 is involved in the incidence and progression of febrile seizures. PMID: 28372289
DPP-4 inhibition with linagliptin may represent a novel treatment for chronic kidney disease. PMID: 27083282
DPP4 inhibition protects against ventricular arrhythmias by attenuating NGF-induced sympathetic innervation via cAMP/PKA/CREB-dependent antioxidant pathway in non-diabetic infarcted rats. PMID: 26399925
Dipeptidyl peptidase-4 inhibition by gemigliptin exerts a preventative effect on the proliferation and migration of VSMCs via Nrf2. PMID: 26187356
Differences in Expression of DPP4 in Steatotic Rat Liver Are Not Related to Differences in the Methylation of its Gene Promoter PMID: 26359413
The inhibition of the renal DPP-4 activity induced by saxagliptin may contribute to ameliorating renal injury in hypertension-related renal injury. PMID: 25936515
Inhibition of DPP-4 reduces T2DM-induced increase in post-MI acute mortality possibly by restoring the autophagic response through attenuation of Bcl-2-Beclin-1 interaction. PMID: 26259714
DPP4 inhibitor vildagliptin attenuates oxidative stress and cardiac fibrosis and improving cardiac function in rats with chronic myocardial infarction. PMID: 25823534
Findings indicate a positive correlation between reduced stress-responsiveness and increased central NPY, in DPP4mut rats. PMID: 25635612
DPP-4 inhibitors reduced macrophage infiltration via glucagon-like peptide-1-dependent signaling in nephritis model and suggest that the control of inflammation by DPP-4 inhibitors is useful for the treatment of nondiabetic kidney disease models. PMID: 25656369
bone marrow derived cells but not the kidney represent at least one source of sDPP4. And leukocyte or macrophage subpopulations could be potential candidates. PMID: 24874705
DPP4 regulates the expression of the hemoglobin genes, and might play a role in the preservation of renal function. PMID: 25122001
Maternal deprivation as a model for postnatal stress experience influences remarkably postnatal lung development of rats, which is significantly modulated by the DPP4-system. PMID: 24357522
Long term deficiency of DPP4 activity improved cardiac performance against pressure overload in rat. PMID: 24416433
a low level of DPPIV activity contributed to maintaining intestinal homeostasis PMID: 23832365
Circulating DPPIV activity is associated with poorer cardiovascular outcomes in heart failure. PMID: 23894014
High fat and high cholesterol diets increased DPP-IV expression in intestinal lymph. PMID: 23535306
DPP-IV is a promising in situ marker of biliary functionality not only of normal but also of fatty rats. PMID: 23361237
Long term loss of DPP4 activity increased the capability against ROS stress, which was more than GLP-1 dependent pathway PMID: 23359639
DPP-4 deficiency restored ischemia (but not G-CSF)-induced stem cell mobilization and improved vascular recovery in diabetic animals PMID: 23184393
These data suggest that GLP-1 analogs may serve as a novel therapeutic drug to alleviate obesity-induced liver injury by reducing bile acid synthesis and improving liver bile secretory function. PMID: 22918684
Dipeptidyl peptidase IV regulates proliferation of preglomerular vascular smooth muscle and mesangial cells. PMID: 22802229
novel evidence shows the regulatory roles of DPP4 in chronic heart failure. PMID: 23035207
Seven days post artery occlusion, dipeptidyl peptidase IV immunoreactivity was found in the perikarya of surviving cortical neurons of the ipsilateral hemisphere. PMID: 22373413
APN and DPPIV activity levels are related to the development of arthritis, being protective or inducer of the susceptibility PMID: 21982785
This study demonstrates a reduction of anabolic effects on the energy homeostasis and a centrally enhanced NPY-expression in DPP4-deficient rats under high caloric food intake. DPP4-deficiency altered intracorporal fat distribution. PMID: 20887754
plasma DPP4 activity changes in accordance with the progression of hyperinsulinemic obesity and pancreatic islet atrophy PMID: 21139073
Monosodium glutamate and/or food deprivation decreased the activity of dipeptidyl peptidase 4 in the soluble and membrane fraction from the hypothalamus, and in the membrane fraction from the hippocampus. PMID: 20153005
Reduced airway inflammation in CD26-deficient F344 rats appear to be mediated by differences in the recruitment and activity of Tregs. PMID: 20560982
APM and DPPIV-DI are respectively related to the downregulation of somatostatin in food-deprived rats, and to the recovery of energy balance in monosodium glutamate obese rats during food deprivation PMID: 19876009
These findings demonstrate a negative regulatory role of the bronchus-associated lymphatic tissue -specific expression of CD26 in T-cell adhesion during an asthma-like inflammation. PMID: 19501934
The 6A3 epitope was stably exposed in both native and denatured rDPP IV. Data mapped the 6A3 epitope to a surface-exposed Thr331-dependent motif D329KTTLVWN, only 11 amino acids away from L311QWLRRI on the same plane as the fifth beta-propeller blade. PMID: 19804410
Immunoreactive endomorphin 2 was generated extracellularly by a membrane-bound DPP-IV, which was switched to "synthase" mode by the hydrolase inhibitor Ile-Pro-Ile PMID: 19540879
Clustered charged amino acids of human adenosine deaminase comprise a functional epitope for binding the adenosine deaminase complexing protein CD26/dipeptidyl peptidase IV. PMID: 11901152
DPPIV deficiency results in improved glucose tolerance and ameliorated insulin resistance PMID: 12031691
kinetics of this enzyme and insights into its dimerization and gelatinase activity PMID: 12675219
An increase in this enzyme is associated with graft rejection. Inhibition of this enzyme prevents heart graft rejection. PMID: 12675233
The three-dimensional structure of DPPIV as determined by cryo-TEM and single particle analysis. PMID: 12705886
identification of DPPIV-binding sites in fibronectin and the effect of binding site peptides on DPPIV/poly-fibronectin adhesion and metastasis PMID: 12716896
The behavioral repertoire and response to ethanol was characterized in dipeptidyl peptidase IV (DPPIV/CD26)-deficient, mutant Japanese, German, and wild-type-like F344 rat substrains. PMID: 14568317
presence of a yet to be characterized signalling mechanism whereby DPP IV has access to c-Src-containing signalling platforms PMID: 16478473
short-term DP IV inhibition does not eliminate the satiety actions of exogenously administered peptide YY at the doses tested. PMID: 16802131
results extend earlier findings and illustrate the role of CD26/DPP IV in alloantigen-mediated immune responses PMID: 16962474
The X-ray structure confirms that the binding mode of rat DPPIV is similar to the parent xanthines. PMID: 17010607
Inhibition of intragraft DPP IV activity significantly reduced ischemia/reperfusion-associated pulmonary injury, allowing for successful transplantation after 18 hours of ischemia. PMID: 17175274
Rats with a high fat diet significantly increased DPP-IV's expression and activity about 142-152% in the intestine and 153-240% in kidneys, but there was no change in the liver. PMID: 17583752

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Subcellular Location

[Dipeptidyl peptidase 4 soluble form]: Secreted.; Cell membrane; Single-pass type II membrane protein. Apical cell membrane; Single-pass type II membrane protein. Cell projection, invadopodium membrane; Single-pass type II membrane protein. Cell projection, lamellipodium membrane; Single-pass type II membrane protein. Cell junction. Membrane raft.

Protein Families

Peptidase S9B family, DPPIV subfamily

Tissue Specificity

Expressed in bile ducts and other epithelial brush borders (small intestine, kidney, colon, pancreatic duct); acinar structures in salivary glands; endothelial structures and T cell areas in thymus, spleen and lymph node.

Database Links

KEGG: rno:25253

STRING: 10116.ENSRNOP00000045536

UniGene: Rn.91364

Code	CSB-EP007139RA
Abbreviation	Recombinant Rat Dpp4 protein, partial
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Rat Dipeptidyl peptidase 4 (Dpp4), partial

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