Just like other recombinant proteins, the production of this recombinant Rat Dpp4 protein began with appropriate cDNA and PCR methods, and then the Dpp4 expression plasmids were built. Following sequence determination of the constructs, plasmids were transformed into E.coli for the expression of the recombinant Rat Dpp4 protein. N-terminal 6xHis-SUMO tag was used in the process. And we finally get the protein of interest with purity of 90%+.
Dpp4 is a gene providing an instruction of making a protein named dipeptidyl peptidase 4 in rattus norvegicus (rat). Dipeptidyl peptidase 4 is also known as bile canaliculus domain-specific membrane glycoprotein, dipeptidyl peptidase IV (DPP IV), GP110 glycoprotein and T-cell activation antigen CD26 (CD26). This protein can be cleaved into 3 chains, including dipeptidyl peptidase 4 membrane form, dipeptidyl peptidase 4 soluble form and dipeptidyl peptidase 4 60 kDa soluble form. It is involved in many biological processes, such as cell adhesion, endothelial cell migration, T cell activation, etc.
||Greater than 90% as determined by SDS-PAGE.
||Dpp4; Cd26; Dipeptidyl peptidase 4; Bile canaliculus domain-specific membrane glycoprotein; Dipeptidyl peptidase IV; DPP IV; GP110 glycoprotein; T-cell activation antigen CD26; CD antigen CD26
||Rattus norvegicus (Rat)
|Target Protein Sequence
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
|| If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
||3-7 business days
||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
|Datasheet & COA
|| Please contact us to get it.