Rat macrophage inflammatory protein 1α,MIP-1α ELISA Kit

Product Details

Related Products

Customer Reviews and Q&A

Target Background

Product Details

Alternative Names

Ccl3 ELISA Kit; Mip1a ELISA Kit; Scya3 ELISA Kit; C-C motif chemokine 3 ELISA Kit; Macrophage inflammatory protein 1-alpha ELISA Kit; MIP-1-alpha ELISA Kit; Small-inducible cytokine A3 ELISA Kit

Abbreviation

CCL3

Uniprot No.

P50229

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

3.9 pg/mL-250 pg/mL

Sensitivity

1.95 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-99
1:2	Average %	101
	Range %	94-108
1:4	Average %	97
	Range %	92-102
1:8	Average %	93
	Range %	89-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

250

2.722

2.634

2.678

2.550

125

2.023

2.087

2.055

1.927

62.5

1.236

1.268

1.252

1.124

31.25

0.765

0.729

0.747

0.619

15.6

0.514

0.501

0.507

0.379

7.8

0.286

0.273

0.280

0.152

3.9

0.225

0.214

0.219

0.091

0.131

0.125

0.128

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Description

This ELISA kit provides quantitative measurement of CCL3 (MIP-1α) in rat samples, supporting immunology research focused on macrophage-driven inflammatory signaling and leukocyte recruitment. Because CCL3 is rapidly secreted at low concentrations during early innate immune activation and varies dramatically between resting and stimulated conditions, researchers need an assay calibrated to capture this dynamic expression window.

Detection Range: The 3.9–250 pg/mL range spans CCL3 concentrations typically observed from baseline levels in naive rat serum to elevated levels in LPS-challenged or injury models, reducing the need for extensive sample dilution in most acute inflammation experiments.

Sensitivity: At 1.95 pg/mL, the kit detects CCL3 in unstimulated macrophage supernatants and resting-state plasma, where this chemokine is often present at single-digit pg/mL concentrations before inflammatory challenge.

Sample Compatibility: Validation across serum, plasma, cell culture supernatants, and tissue homogenates enables researchers to correlate circulating CCL3 with local tissue expression and in vitro macrophage or microglial secretion within a single experimental framework.

Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate primary rat macrophage cultures and small-volume plasma draws from serial bleeding protocols, preserving limited biological material for parallel analyses.

This kit is directly applicable to rat models of sepsis, neuroinflammation, and hepatic ischemia-reperfusion injury.

Target Background

Function
(From Uniprot)

Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.

Gene References into Functions

The important chemokine macrophage inflammatory protein1alpha increases inflammatory responses and the secretion of inflammatory molecules from Kupffer cells, including tumor necrosis factoralpha, interleukin (IL)1beta and IL6. PMID: 28677732
The present report demonstrates an increase in mRNA and protein levels in chemokines CCL3 and its receptor CCR5 in dorsal root ganglion in the rat model of remifentanil-induced hyperalgesia. Furthermore, the results pointed out that CCL3 and its receptor CCR5 might be implicated in remifentanil-induced hyperalgesia. PMID: 26550961
Study shows that erythropoietin reduced chemokine CCL3 mRNA expression in rat cerebellar neuron-enriched preparations during neuroinflammation. PMID: 26178913
The results of this study suggest a contribution of CCL3 in the spinal dorsal horn to paclitaxel-induced allodynia and may provide new therapeutic targets for paclitaxel-induced painful neuropathy. PMID: 25127716
Expression levels of CCL3 in the spinal cord are up-regulated following nerve injury. PMID: 24589480
NMDA-evoked neuronal injury differentially induces MIP-1alpha and MCP-1 production in microglia and astrocytes, respectively, through activation of different intracellular signaling pathways. PMID: 22791363
Our data suggest that free radicals produced in CA1 neurons contribute to the recruitment and activation of microglia and neurodegeneration through MIP-1alpha expression. PMID: 18272692
In this study we present data on long term increase in CCL3 mRNA expression and immunoreactivity following epileptogenic stimulus in a rat model of temporal lobe epilepsy. PMID: 21731074
Data show significant concentration increases for CXCL1 and MIP-1alpha in the brain after soman-induced seizure onset. PMID: 21535896
The active behavior of the MIP-1alpha gene in KCs following liver ischemia/reperfusion injury is assumed to be one of the major causes for the hepatic ischemia/reperfusion injury. PMID: 16804970
P2Y6 activation by uridine 5'-diphosphate induces Ccl2 and Ccl3 expression in primary cultured microglia, astrocytes, and brain slices from neonatal and adult rats. PMID: 21317391
a massive amount of ATP molecules released in the glioma tumor site may act as the regulator with P2XR signaling that increases MIP-1alpha and MCP-1 expression in tumor-infiltrating microglia/macrophages. PMID: 21162127
These results suggest that MIP-1alpha is directly involved in the neurodegeneration induced in rats by PVC-211 murine leukemia virus by recruiting macrophages/microglia from the periphery into regions of the brain that eventually become diseased. PMID: 21051067
expression is regulated by nicotine in alveolar macrophages PMID: 12101081
The constitutive patterns of MIP-1 alpha mRNA and protein expression are demonstrated in rat brain throughout postnatal development within local microenvironments where the postnatal cell migration phenomenon occurs. PMID: 12899200
Oligodendroglia express the CCL3 receptor CCR1, but not CCR5. CCL3 induced an intracellular rise in Ca(2+), a typical signalling event of G protein-coupled receptors. PMID: 14583384

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Subcellular Location

Secreted.

Protein Families

Intercrine beta (chemokine CC) family

Database Links

KEGG: rno:25542

STRING: 10116.ENSRNOP00000015139

UniGene: Rn.10139

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Code	CSB-E07422r-IS
Size	96T,5×96T,10×96T
Price	Request a Quote
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.