Rat macrophage inflammatory protein 1α,MIP-1α ELISA Kit

Code CSB-E07422r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
chemokine (C-C motif) ligand 3
Alternative Names
Ccl3 ELISA Kit; Mip1a ELISA Kit; Scya3 ELISA Kit; C-C motif chemokine 3 ELISA Kit; Macrophage inflammatory protein 1-alpha ELISA Kit; MIP-1-alpha ELISA Kit; Small-inducible cytokine A3 ELISA Kit
Abbreviation
CCL3
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
12.5 pg/mL-800 pg/mL
Sensitivity
3.12 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 84-98  
1:2 Average % 96  
Range % 91-104  
1:4 Average % 99  
Range % 96-105  
1:8 Average % 95  
Range % 89-99  
Recovery
The recovery of rat MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 104 99-108  
EDTA plasma (n=4) 95 91-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
800 2.152 2.051 2.102 1.969  
400 1.484 1.383 1.434 1.301  
200 0.888 0.867 0.878 0.745  
100 0.522 0.511 0.517 0.384  
50 0.336 0.347 0.342 0.209  
25 0.253 0.264 0.259 0.126  
12.5 0.195 0.199 0.197 0.064  
0 0.132 0.133 0.133    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat CCL3 ELISA Kit was designed for the quantitative measurement of Rat CCL3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL .

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.
Gene References into Functions
  1. The important chemokine macrophage inflammatory protein1alpha increases inflammatory responses and the secretion of inflammatory molecules from Kupffer cells, including tumor necrosis factoralpha, interleukin (IL)1beta and IL6. PMID: 28677732
  2. The present report demonstrates an increase in mRNA and protein levels in chemokines CCL3 and its receptor CCR5 in dorsal root ganglion in the rat model of remifentanil-induced hyperalgesia. Furthermore, the results pointed out that CCL3 and its receptor CCR5 might be implicated in remifentanil-induced hyperalgesia. PMID: 26550961
  3. Study shows that erythropoietin reduced chemokine CCL3 mRNA expression in rat cerebellar neuron-enriched preparations during neuroinflammation. PMID: 26178913
  4. The results of this study suggest a contribution of CCL3 in the spinal dorsal horn to paclitaxel-induced allodynia and may provide new therapeutic targets for paclitaxel-induced painful neuropathy. PMID: 25127716
  5. Expression levels of CCL3 in the spinal cord are up-regulated following nerve injury. PMID: 24589480
  6. NMDA-evoked neuronal injury differentially induces MIP-1alpha and MCP-1 production in microglia and astrocytes, respectively, through activation of different intracellular signaling pathways. PMID: 22791363
  7. Our data suggest that free radicals produced in CA1 neurons contribute to the recruitment and activation of microglia and neurodegeneration through MIP-1alpha expression. PMID: 18272692
  8. In this study we present data on long term increase in CCL3 mRNA expression and immunoreactivity following epileptogenic stimulus in a rat model of temporal lobe epilepsy. PMID: 21731074
  9. Data show significant concentration increases for CXCL1 and MIP-1alpha in the brain after soman-induced seizure onset. PMID: 21535896
  10. The active behavior of the MIP-1alpha gene in KCs following liver ischemia/reperfusion injury is assumed to be one of the major causes for the hepatic ischemia/reperfusion injury. PMID: 16804970
  11. P2Y6 activation by uridine 5'-diphosphate induces Ccl2 and Ccl3 expression in primary cultured microglia, astrocytes, and brain slices from neonatal and adult rats. PMID: 21317391
  12. a massive amount of ATP molecules released in the glioma tumor site may act as the regulator with P2XR signaling that increases MIP-1alpha and MCP-1 expression in tumor-infiltrating microglia/macrophages. PMID: 21162127
  13. These results suggest that MIP-1alpha is directly involved in the neurodegeneration induced in rats by PVC-211 murine leukemia virus by recruiting macrophages/microglia from the periphery into regions of the brain that eventually become diseased. PMID: 21051067
  14. expression is regulated by nicotine in alveolar macrophages PMID: 12101081
  15. The constitutive patterns of MIP-1 alpha mRNA and protein expression are demonstrated in rat brain throughout postnatal development within local microenvironments where the postnatal cell migration phenomenon occurs. PMID: 12899200
  16. Oligodendroglia express the CCL3 receptor CCR1, but not CCR5. CCL3 induced an intracellular rise in Ca(2+), a typical signalling event of G protein-coupled receptors. PMID: 14583384

Show More

Hide All

Subcellular Location
Secreted.
Protein Families
Intercrine beta (chemokine CC) family
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X