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Rat oncostatin M (OSM) ELISA kit

Protocol
Code CSB-E07402r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name oncostatin M
Alternative Names Osm ELISA Kit; Oncostatin-M ELISA Kit; OSM ELISA Kit
Abbreviation OSM
Uniprot No. Q65Z15
Species Rattus norvegicus (Rat)
Sample Types serum, plasma
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat OSM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)
1:1 Average % 91
Range % 86-95
1:2 Average % 104
Range % 99-108
1:4 Average % 91
Range % 85-97
1:8 Average % 95
Range % 91-99
Recovery
The recovery of rat OSM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 92-102
EDTA plasma (n=4) 89 82-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.877 2.986 2.932 2.828
500 2.315 2.421 2.368 2.264
250 1.531 1.567 1.549 1.445
125 0.818 0.824 0.821 0.717
62.5 0.403 0.412 0.408 0.304
31.25 0.272 0.265 0.269 0.165
15.6 0.206 0.202 0.204 0.100
0 0.103 0.104 0.104 　
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Growth regulator. Inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses only type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.
Gene References into Functions
  1. OSM is a key mediator for inducing differentiation of OC15-5 cells into hepatocytes PMID: 15743783
  2. In the immortalized mouse and primary cultured proliferative rat hepatocytes, treatment with OSM markedly increased mRNA and protein of claudin-2 together with formation of developed networks of TJ strands. PMID: 17434483
  3. OSM may not only play a role in the regulation of Sertoli cell proliferation and the initiation of spermatogenesis but may also play a role in the regulation of Leydig cell progenitor formation. PMID: 17996055
  4. Results indicate that osteosarcoma cells stably producing OSM do not develop resistance to this cytokine and thus could be a valuable new tool to study the anti-cancer effect of OSM in vivo. PMID: 19168167
Subcellular Location Secreted.
Protein Families LIF/OSM family
Tissue Specificity Widely expressed. Expressed at higher levels in liver, skin and spleen.
Database Links

KEGG: rno:289747

STRING: 10116.ENSRNOP00000032548

UniGene: Rn.127158
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