OSM

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human OSM at 2μg/mL can bind Anti-OSM recombinant antibody (CSB-RA017260MA1HU)，the EC₅₀ is 3.048-3.860 ng/mL.

OSM Recombinant Monoclonal Antibody (CSB-RA017260MA1HU) captured on Protein A Chip can bind Recombinant Human OSM protein with an affinity constant of 0.59 nM as detected by MetaSPR Assay (WeSPRTM 200).

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

The Binding Activity of Human OSM with Anti-OSM Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human OSM (CSB-MP017260HU1) at 2 μg/mL can bind Anti-OSM recombinant antibody, the EC₅₀ is 3.048-3.860 ng/mL.

IHC image of CSB-PA09694A0Rb diluted at 1:90 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA09694A0Rb diluted at 1:90 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.