Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit

Code CSB-E07398r
Size 96T,5×96T,10×96T
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Product Details

Target Name
regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5)
Alternative Names
Ccl5 ELISA Kit; Scya5C-C motif chemokine 5 ELISA Kit; SIS-delta ELISA Kit; Small-inducible cytokine A5 ELISA Kit; T-cell-specific protein RANTES ELISA Kit
Abbreviation
RANTES/CCL5
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.156 ng/mL-10 ng/mL
Sensitivity
0.039 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat RANTES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 99
Range % 94-105
1:2 Average % 102
Range % 97-107
1:4 Average % 92
Range % 88-96
1:8 Average % 101
Range % 97-106
Recovery
The recovery of rat RANTES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 87 83-92
EDTA plasma (n=4) 92 86-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.684 2.799 2.742 2.633
5 2.178 2.349 2.264 2.155
2.5 1.444 1.489 1.467 1.358
1.25 0.783 0.795 0.789 0.680
0.625 0.376 0.392 0.384 0.275
0.312 0.258 0.264 0.261 0.152
0.156 0.201 0.208 0.205 0.096
0 0.108 0.109 0.109  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The Rat Ccl5 ELISA Kit is a reliable and precise tool for the detection and quantification of C-C motif chemokine 5 (Ccl5) in rat serum, plasma, cell culture supernates, and tissue homogenates. Ccl5 is an important protein that is involved in a variety of immune responses, including inflammation and the recruitment of immune cells to sites of infection.

This ELISA kit features a sensitive detection range of 0.156 ng/mL to 10 ng/mL, with a sensitivity of 0.039 ng/mL. The assay principle is based on the sandwich measurement technique, providing researchers with quantitative results in as little as 1-5 hours. The kit is optimized for use with a sample volume of 50-100ul, making it a good solution for researchers.

The Rat Ccl5 ELISA Kit is an ideal choice for researchers investigating immunology, inflammation, and immune responses in rats. With its sensitive detection range, efficient assay time, and cost-effective design, this kit is a valuable addition to any researcher's toolkit.

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Target Background

Function
(From Uniprot)
Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
Subcellular Location
Secreted.
Protein Families
Intercrine beta (chemokine CC) family
Database Links
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