Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit

This ELISA kit provides quantitative measurement of CCL5 (RANTES) in rat samples, supporting immunology research focused on chemokine-driven leukocyte recruitment and inflammatory signaling. Because CCL5 concentrations can vary dramatically between resting and activated states — often spanning orders of magnitude during acute inflammation or tissue injury — this kit is designed to capture that dynamic range while accommodating diverse experimental models.

Detection Range: The 15.6–1000 pg/mL range covers CCL5 levels typically observed in rat serum and plasma during both baseline and inflammatory conditions, including LPS challenge and ischemia-reperfusion models. Samples from highly stimulated conditions may require dilution, while unstimulated controls generally fall within range without concentration.

Sensitivity: At 7.8 pg/mL, the kit detects CCL5 in low-abundance samples such as conditioned media from unstimulated macrophages or early-phase tissue homogenates, where chemokine secretion has not yet peaked.

Sample Compatibility: Validation across serum, plasma, cell culture supernatants, and tissue homogenates enables researchers to correlate circulating CCL5 levels with local tissue expression and in vitro cellular responses within a single study.

Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate primary cell experiments where supernatant volumes are limited and time-course sampling demands efficient reagent use.

This kit is directly applicable to studies of neuroinflammation, transplant rejection, and arthritis modeling in rat systems.