Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit

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Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit

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Protocol

Code	CSB-E07398r-IS
Size	96T,5×96T,10×96T
Price	Request a Quote
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Target Background

Alternative Names

Ccl5 ELISA Kit; Scya5C-C motif chemokine 5 ELISA Kit; SIS-delta ELISA Kit; Small-inducible cytokine A5 ELISA Kit; T-cell-specific protein RANTES ELISA Kit

Abbreviation

CCL5

Uniprot No.

P50231

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

7.8 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat RANTES/CCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:10	Average %	99
	Range %	92-105
1:20	Average %	93
	Range %	89-98
1:40	Average %	101
	Range %	96-106
1:80	Average %	90
	Range %	85-96

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

1000

2.863

2.914

2.888

2.738

500

1.685

1.617

1.651

1.501

250

0.772

0.738

0.755

0.605

125

0.445

0.429

0.437

0.287

62.5

0.313

0.315

0.314

0.164

31.2

0.225

0.233

0.229

0.079

15.6

0.196

0.189

0.192

0.042

0.152

0.148

0.150

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.

Subcellular Location

Secreted.

Protein Families

Intercrine beta (chemokine CC) family

Database Links

STRING: 10116.ENSRNOP00000014865

UniGene: Rn.8019

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Email: [email protected]
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Phage Display Service

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Related pathways

Chemokine signaling pathway TNF signaling pathway NOD-like receptor signaling pathway Toll-like receptor signaling pathway

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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