Code | CSB-E11807h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human Lgals3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 88 | ||||
Range % | 83-95 | |||||
1:2 | Average % | 98 | ||||
Range % | 91-104 | |||||
1:4 | Average % | 92 | ||||
Range % | 87-96 | |||||
1:8 | Average % | 93 | ||||
Range % | 88-100 |
The recovery of human Lgals3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 93 | 86-97 | ||||
EDTA plasma (n=4) | 102 | 94-107 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The human LGALS3 ELISA kit (CSB-E11807h) is designed for the quantitative measurement of human LGALS3 protein in serum, plasma, cell culture supernates, tissue homogenates, or saliva. It quantitates human LGALS3 with 0.156 ng/ml sensitivity and shows excellent specificity for human LGALS3. It uses the bi-antibody sandwich enzyme immunoassay technique. This assay employs a biotin-conjugated LGALS3 antibody that recognizes the analyte bound by the immobilized LGALS3 antibody, forming an antibody-analyte-antibody complex. The immune complex is further detected by avidin-conjugated HRP. The TMB solution is added into the wells and turns blue and finally turns yellow after the addition of the stop solution. Solution color develops in proportion to the amount of LGALS3 in the sample. The O.D. value is measured using a microplate reader at 450 nm and is used to determine the concentration of the human LGALS3 in the sample.
LGALS3 is a β-galactoside binding protein involved in multiple cellular processes that support cell growth and cell survival. In the context of the entire tumor microenvironment, LGALS3 functions as a tumor promoter by promoting the survival of malignant cells, supporting metastasis, inhibiting immune surveillance, and regulating the inflammatory expression of chemokines/cytokines. LGALS3 is also regarded as a potent pro-inflammatory signaling factor, playing an important role in the activation, chemotaxis, and cytokine release of inflammatory cells. Levels of circulating LGALS3 are increased in chronic inflammatory diseases including obesity, diabetes, and its complications.
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I kindly ask you to inform if you have human Galectin- 3 Elisa Kit.