LGALS3 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA970289A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg), HT29 whole cell lysate(30µg), U87 whole cell lysate(30µg), CACO2 whole cell lysate(30µg), BxPC-3 whole cell lysate(30µg),A431 whole cell lysate(30µg),A549 whole cell lysate(30µg) All lanes: LGALS3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 10s IHC image of CSB-RA970289A0HU diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody Immunofluorescence staining of MCF7 cell with CSB-RA970289A0HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunoprecipitating LGALS3 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA970289A0HU in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA970289A0HU(2µg)+ Hela whole cell lysate(500µg) Lane 3: Hela whole cell lysate (10µg) Immunofluorescence staining of MCF7 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of Hela cell with CSB-RA970289A0HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Uniprot No.

P17931

Target Names

LGALS3

Alternative Names

Galectin-3 (Gal-3) (35 kDa lectin) (Carbohydrate-binding protein 35) (CBP 35) (Galactose-specific lectin 3) (Galactoside-binding protein) (GALBP) (IgE-binding protein) (L-31) (Laminin-binding protein) (Lectin L-29) (Mac-2 antigen), LGALS3, MAC2

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human Galectin 3

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4E1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200
IP	1:200-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Galectin-3, encoded by the LGALS3 gene, is a versatile beta-galactoside-binding lectin that plays critical roles in cell adhesion, proliferation, apoptosis, and immune regulation. This multifunctional protein has emerged as a significant research target in neuroscience and cell biology, with particular relevance to studies of inflammation, fibrosis, and cancer progression where its expression patterns often correlate with disease states.

This recombinant monoclonal antibody, clone 4E1, offers the consistency and reliability that demanding research protocols require. Produced using recombinant technology in rabbit host, it provides sequence-defined specificity against human Galectin-3, ensuring reproducible results across experiments and eliminating the lot-to-lot variability that can compromise longitudinal studies.

Extensive validation demonstrates this antibody's versatility across multiple experimental platforms. Western blot analysis confirms robust detection in diverse human cell lines including MCF-7, HeLa, Colo320, and HT29, as well as mouse NIH/3T3 cells, with an observed band at approximately 31 kDa. The slight difference from the predicted 27 kDa molecular weight likely reflects post-translational modifications, particularly glycosylation, which is characteristic of this lectin family member. Immunohistochemistry validation in paraffin-embedded human ovarian cancer tissue demonstrates clear staining with standard citrate buffer antigen retrieval protocols. Immunofluorescence studies in MCF-7 cells reveal distinct cellular localization patterns, while immunoprecipitation from HeLa lysates confirms the antibody's capacity for protein complex isolation studies.

Whether investigating Galectin-3's role in tumor microenvironment modulation, neuroinflammatory processes, or cellular signaling pathways, this antibody provides researchers with a dependable tool supported by comprehensive application data across immunoassay, blotting, imaging, and protein interaction workflows.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.

Gene References into Functions

results demonstrate that human resting NK cells express Gal-3 at both gene and protein levels and that the Gal-3 expression can be modulated upon cytokine stimulation. In the same cells, Gal-3 always localizes intracellularly and functionally correlates with the degree of NK cell degranulation. PMID: 29248489
Interaction between galectin-3 and its potential inhibitor, bergenin, was studied using computational methods. PMID: 30276553
It has been reported that the Galectin-3/NuMA interaction is functionally important for the spindle pole organization; spindle pole cohesion requires glycosylation-mediated localization of NuMA. PMID: 28469279
The Mac-2-binding protein glycosylation isomer (M2BPGi), which is also known as Wisteria floribunda agglutinin-positive human Mac-2-binding protein, was recently established as a glycol-biomarker of liver fibrosis in patients with chronic hepatitis C with a unique fibrosis-related glycoalteration. PMID: 30128700
Higher plasma galectin-3 levels were associated with an elevated risk of developing incident chronic kidney disease, particularly among those with hypertension. PMID: 28865675
Pre-interventional plasma Galectin-3 levels are associated with left ventricular reverse remodeling and with clinical outcome after percutaneous mitral valve repair. PMID: 29678512
Galectin-3 is significantly associated with functional capacity, cardiac function and adverse cardiovascular events in adults with congenital heart defects. PMID: 28942393
Gene expression was analyzed for miR-1, miR-21, and galectin-3 in hypertensive patients with symptomatic heart failure and left ventricular hypertrophy. PMID: 29905364
Serum expression of miR-1 and miR-21, and the concentration of gal-3 in systolic heart failure patients with different degrees of left ventricular dilatation. PMID: 29905365
Serum galectin-3 is associated with coronary atherosclerosis and obstructive sleep apnoea syndrome (OSAS) severity in OSAS patients. PMID: 28150280
Study demonstrates that mesenchymal stromal cells (MSC)-derived LGALS3 may be critical for important biological pathways for MSC homeostasis and for regulating AML cell localization and survival in the leukemia microenvironmental niche. PMID: 29655803
Low expression of galectin-3 was detected in all patients with malignant gastrointestinal tumors irrespective of the presence of eosinophilia. PMID: 29926280
These findings highlight a new role for Galectin 3 as a non-classic RNA-binding protein that regulates MUC4 mRNA post-transcriptionally. PMID: 28262838
Galectin-3 is superior to sST2 in distinguishing HFpEF from controls and HFrEF. PMID: 30039808
erum Gal-3 levels were significantly higher in breast cancer (BC) patients and did not significantly differ according to clinical and tumoral characteristics of patients. Furthermore, there was no difference in Gal-3 levels between breast patients with and without metastatic disease. PMID: 30249872
Gal3 expression in regional lymph nodes might be associated with oral squamous cell carcinomas progression. The increased Gal3 expression in regional lymph nodes of larger tumors underlines the need of immunomodulatory treatment concepts in early-stage oral squamous cell carcinomas . Blocking of Gal3 might be a therapeutic option in oral cancer. PMID: 30115022
High Gal-3 serum levels predict fibrosis of the atrial appendage. PMID: 28079145
this study reviews the role of galectin-3 in Atrial Fibrillation mechanisms and its potential therapeutic implications. PMID: 29587379
Our findings provide evidence that allele C of rs4652 and allele T of rs11125 in the galectin-3 gene may be risk factors for cervical cancer. PMID: 28848207
High GAL-3 expression is associated with Salivary Gland Tumor. PMID: 29580041
Galectin-3 is greatly reduced in patients with cutaneous lupus lesions compared with healthy controls, which may contribute to the recruitment of inflammatory cells in the skin. PMID: 29058991
Progesterone receptor, EGFR, and galectin-3 are expressed differentially in uterine smooth muscle tumors. PMID: 29729689
Galectin-3 belongs to a class of inflammatory mediators that is associated with the degree of myocardial inflammation and fibrosis. It is related to the severity of myocardial ischemia and is negatively correlated with the cardiac ejection fraction. PMID: 29287900
Increased GAL-3 levels are an independent predictor of all-cause mortality in hemodialysis patients. PMID: 26787685
High GAL3 expression is associated with oral squamous cell carcinomas. PMID: 29284429
High expression level of Galectin-3 and low expression level of TRAIL were found to be positively correlated with the shorter median survival time and overall survival time. PMID: 28925481
Galectin-3 is overexpressed in patients with thyroid nodules, and can be used for diagnostic imaging and therapeutic targeting of cancer patients. (Review) PMID: 29393868
With emerging evidence to support the function and application of galectin3, the current review aims to summarize the latest literature regarding the biomarker characteristics and potential therapeutic application of galectin3 in associated diseases. PMID: 29207027
Our results indicate that concomitant stimulation and colocalization of galectin-3 with CD147 are associated with increased gelatinolytic activity in the actively ulcerating human cornea PMID: 29340650
Galectin-3 can be used as a biomarker for the prognosis evaluation of acute heart failure, and its combined analysis can increase the predictive value of NT-proBNP PMID: 29077153
Galectin has been identified as a proinflammatory protein and modulates immune responses either as damage-associated molecular patterns or as pattern recognition receptors. PMID: 28828226
Gal-3 was found to be significantly higher in the stroma of patients with H. pylori infection, mainly on Cag-A positive H. pylori, with chronic gastritis. PMID: 28939284
The present study demonstrates a relationship between galectin-3 levels and total body fat, abdominal fat, body fat distribution, cardiac size and geometry, and increase in total body fat over 2 years in young children. PMID: 29327139
Results suggest that galectin-3 could help to monitor the risk of short-term mortality in unselected patients with acute heart failure (AHF) attended in the emergency department (ED). PMID: 28406038
Report establishes the functional significance of Gal-3 as a broad-spectrum upstream effector in osteoarthritis (OA). A positive correlation between cartilage degeneration and Gal-3 positivity in chondrocytes and the ECM was found. PMID: 27982117
the secretion of galectin-3 as a novel mechanism for osteoblasts to control osteoclastogenesis and to maintain trabecular bone homeostasis independently of the RANKL/OPG-axis. PMID: 28822790
Gal-3 plasma concentration was significantly higher in pulmonary arterial hypertension patients. PMID: 28826890
Galectin-3 was associated with incident ischaemic stroke in younger but not older individuals. PMID: 28872212
As MUC1 and galectin-3 are both commonly overexpressed in most types of epithelial cancers, their interaction and impact on EGFR activation likely makes important contribution to EGFR-associated tumorigenesis and cancer progression. PMID: 28731466
serum Gal-3 is increased in Acute exacerbation of chronic obstructive pulmonary disease patients. PMID: 28947730
High serum levels of galectin-3 were found in patients with schizophrenia compared with that in controls. PMID: 28698921
N-linked glycoprotein maturation is not required for Gal-3 transport from the cytosol to the extracellular space, but is important for cell surface binding. Additionally, secreted Gal-3 is predominantly free and not packaged into extracellular vesicles. These data support a secretion pathway independent of N-linked glycoproteins and extracellular vesicles. PMID: 28775154
NRG1 and Gal-3 were significantly more elevated in cancer patients than in healthy controls. PMID: 28430337
discovery of site specific N-glycosylation changes of LGALS3BP in association of PDAC may provide useful clues to facilitate cancer detection or phenotype stratification PMID: 28627758
Our findings establish gal-3 as a molecular regulator of the JAG1/Notch-1 signaling pathway and have direct implications for the development of strategies aimed at controlling tumor angiogenesis. PMID: 28533486
findings reveal that differential levels of Gal-3 and Gal-8 expression and recruitment to Group A streptococcus (GAS) between epithelial cells and endothelial cells may contribute to the different outcomes of GAS elimination or survival and growth of GAS in these two types of cells. PMID: 28743815
The methylation frequency of the galectin-3 promoter was significantly higher while galectin-3 mRNA was lower in acute-on-chronic hepatitis B liver failure (ACHBLF) than in chronic hepatitis B and healthy controls. The results suggest that hypermethylation of the galectin-3 promoter might be an early biomarker for predicting disease severity and prognosis in patients with ACHBLF. PMID: 28185839
Serum Gal-3 and Nox4 levels were significantly elevated and correlated in 26 human pulmonary arterial hypertension patients when compared with 14 age- and sex-matched healthy controls. PMID: 28431936
These results demonstrate Tsc2-deficient mesenchymal progenitors cause aberrant morphogenic signals, and identify an expression signature including Lgals3 relevant for human disease of TSC1/TSC2 inactivation and mTORC1 hyperactivity. PMID: 28695825
Galectin-3 is a possible immunological target molecule of the pathogenic auto-antibodies PMID: 27339072

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Subcellular Location

Cytoplasm. Nucleus. Secreted.

Tissue Specificity

A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes.

Database Links

HGNC: 6563

OMIM: 153619

KEGG: hsa:3958

STRING: 9606.ENSP00000254301

UniGene: Hs.531081

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