Code | CSB-RA970289A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IF | 1:20-1:200 |
IP | 1:200-1:1000 |
The generation of the LGALS3 recombinant monoclonal antibody is based on recombinant DNA technology. This antibody is suitable for detecting human LGALS3 protein in five applications, including ELISA, WB, IHC, IF, and IP. First, the cDNA of the LGALS3 antibody-producing hybridomas is sequenced to synthesize the gene coding for the LGALS3 monoclonal antibody. The hybridomas are created by fusing myeloma cells and B cells isolated from an animal that has been immunized with a synthesized peptide derived from human LGALS3. The synthesized gene is then cloned into a vector and then transfected into cells for cultivation. Finally, the LGALS3 recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography.
The LGALS3 protein is a carbohydrate-binding protein that can be found both inside and outside of cells. It plays a role in various cellular processes, including cell adhesion, apoptosis, and immune response. Specifically, LGALS3 has been shown to be involved in the regulation of cell growth and differentiation, as well as in the modulation of immune cell function. It has also been implicated in the pathogenesis of several diseases, including cancer, fibrosis, and inflammation.
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