Human toll-like receptor 2,TLR2 ELISA Kit

Code CSB-E11781h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O60603
Lead Time 3-5 working days
Abbreviation TLR2
Protein Biological Process 1 Immunity
Target Name toll-like receptor 2
Alias CD282, TIL4, toll/interleukin 1 receptor-like 4
Species Homo sapiens (Human)
Protein Biological Process 3 Immunity
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB.
HGNC 11848
RGD 735138
MGI 1346060
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TLR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %96
Range %89-101
1:2Average %94
Range %88-97
1:4Average %92
Range %85-97
1:8Average %94
Range %88-98
Recovery
The recovery of human TLR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8389-97
EDTA plasma (n=4)9085-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.632 2.621 2.627 2.504
102.068 2.056 2.062 1.939
51.151 1.132 1.142 1.019
2.50.673 0.639 0.656 0.533
1.250.427 0.424 0.426 0.303
0.6250.255 0.245 0.250 0.127
0.3120.189 0.177 0.183 0.060
00.125 0.121 0.123  
References
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Function Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides
Subcellular Location Membrane, Single-pass type I membrane protein, Cytoplasmic vesicle, phagosome membrane, Single-pass type I membrane protein, Membrane raft
Protein Families Toll-like receptor family
Tissue Specificity Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
Database Links

HGNC: 11848

OMIM: 246300

KEGG: hsa:7097

STRING: 9606.ENSP00000260010

UniGene: Hs.519033

Pathway PI3K-Akt signaling pathway
Toll-like receptor signaling pathway

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