Rabbit Thyroid Stimulating Hormone,TSH ELISA Kit

Rabbit Thyroid Stimulating Hormone,TSH ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

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Customer Reviews and Q&A

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Rabbit Thyroid Stimulating Hormone,TSH ELISA Kit

Citations (3) Protocol MSDS

Code	CSB-E06916Rb
Size	96T,5×96T,10×96T
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Product Details
Citations (3)
Related Products
Customer Reviews and Q&A

Target Name

Thyroid Stimulating Hormone,TSH

Abbreviation

TSH

Species

Oryctolagus cuniculus (Rabbit)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.5 μIU/mL-20 μIU/mL

Sensitivity

0.25 μIU/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-104
1:2	Average %	84
	Range %	80-88
1:4	Average %	94
	Range %	90-98
1:8	Average %	92
	Range %	87-96

Recovery

The recovery of rabbit TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	96	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μIU/ml	OD1	OD2	Average
20	2.831	2.836	2.834
10	2.052	2.079	2.066
5	1.178	1.143	1.161
2.5	0.659	0.639	0.649
1	0.260	0.269	0.265
0.5	0.124	0.124	0.124

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Rabbit TSH ELISA Kit was designed for the quantitative measurement of Rabbit TSH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.5 μIU/mL-20 μIU/mL and the sensitivity is 0.25 μIU/mL .

HISTOPATHOLOGICAL CHANGES IN THE NUCLEUS AND CONTRACTILE APPARATUS OF CARDIOMYOCYTES IN OVERT ACQUIRED PRIMARY HYPOTHYROIDISM: EXPERIMENTAL STUDY A Chaulin, J Grigoryeva, E Smirnova, G Suvorova,/,2023
A COMPARATIVE HORMONAL STUDY FOR TWO TYPES OF AMNIOTIC ANIMALS MR Rashied,Iraqi Journal of Agricultural Sciences,2023
Exogenous Neurokinin B Administration May Have a Strong Effect on Negative Feedback Loop of Hypothalamic Pituitary Thyroid Axis Maria Wishal Asmat, et al,International Journal of Peptide Research and Therapeutics,2019

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.