Recombinant Mouse Interleukin-2(Il2) (Active)

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Code CSB-AP004821MO
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 95% as determined by SDS-PAGE.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity The ED50 as determined in a cell proliferation assay using CTLL?2 mouse cytotoxic T cells is less than 0.5 ng/ml.
Target Names Il2
Uniprot No. P04351
Research Area Immunology
Alternative Names Il2; Il-2Interleukin-2; IL-2; T-cell growth factor; TCGF
Species Mus musculus (Mouse)
Source E.coli
Expression Region 21-169aa
Complete Sequence APTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQ
Mol. Weight 17.4 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 μm Filtered 20 mM Sodium Citrate, 5% Trehalose, pH 4.5
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Gene References into Functions
  1. These studies demonstrate that Th1 CD4(+) T cells require IL-2 for lung T resident memory cell development. PMID: 28948612
  2. Because IL-2 production was limited to cells receiving the strongest T cell receptor (TCR) signals, a direct link between TCR-signal strength, IL-2 production, and T cell fate determination has been established. PMID: 30213884
  3. These data highlights the existence of IL-2 trans-presentation between NK cells in the local microenvironment where the availability of IL-2 is limited. PMID: 28074895
  4. interleukin-2 (IL-2) is a non-pancreatic autoimmune target in type 1 diabetes PMID: 27708334
  5. Each mutation decreased STAT5 binding and altered IL-2-induced Il2ra gene expression, revealing that individual elements within the superenhancer were not functionally redundant and that all were required for normal gene expression. PMID: 29078395
  6. Deleting IL-2 in CD11c(high)MHCII(+) cells induces spontaneous colitis resembling human inflammatory bowel disease. PMID: 29549257
  7. a significant increase in plasma levels of IL-2, IFN-g and TNF-g was revealed as assessed by ELISA. In conclusion, the results of the present study indicate that MENK has a cytotoxic effect on B16 melanoma cells in vitro and in vivo, and suggest a potential mechanism for these bioactivities. PMID: 28849104
  8. WASp knockout mice controlled growth of A20 lymphoma cells that naturally produced IL-2. PMID: 27477778
  9. Tumor growth delays observed by tumor irradiation combined with MVA-MUC1-IL-2 vaccine were significantly more prolonged than those observed by vaccine, radiation, or radiation with MVA empty vector. PMID: 28116088
  10. IL-2 signalling is essential to prevent deletion of CD4SP CCR7+ Helios+ thymocytes at a later developmental stage than Card11 is required to prevent deletion. The deletion prevented by IL-2 signalling occurs in a Foxp3-independent manner. PMID: 28362433
  11. in vivo targeting of the TNF superfamily receptor TNFRSF25 using the TL1A-Ig fusion protein, along with IL-2, resulted in transient but massive Treg expansion in donor mice; transplantation of Treg-expanded donor cells facilitated transplant tolerance without GVHD, with complete sparing of graft-versus-malignancy. PMID: 28219835
  12. IL-2 and IL-6 work together to enhance influenza-specific CD8 T cell generation responding to live influenza virus in aged mice and humans PMID: 27322555
  13. this report, we elucidated the unsolved mechanism of the anti-cancer effect of curcumin by identifying IL-2 as a direct molecular target. Curcumin, as a small molecule IL-2 modulator, has the potential to be used to treat IL-2 related pathologic conditions. PMID: 29127008
  14. Data show that PTEN plays a key role in Th17 cell differentiation by blocking IL-2 expression. PMID: 29018045
  15. AnxA6 regulates IL-2 homeostasis and sensitivity in T cells by sustaining a lipid raft-like membrane environment. PMID: 26853809
  16. NOD Treg cells have an impaired responsiveness to IL-2 that reduces their ability to compete for a limited supply of IL-2. PMID: 26763864
  17. Suppression of IL-12p70 formation by IL-2 or following macrophage depletion causes T-cell autoreactivity leading to CNS demyelination in HSV-1-infected mice. PMID: 28542613
  18. At single cell level, IL-2 is binary (digital) and CD25 is graded expressed, whereas at population level both parameters show graded expression correlating with the antigen amount. PMID: 28035902
  19. IL-2/anti-IL-2 complexes protected lupus-prone mice against lupus nephritis by expanding Tregs. PMID: 27914701
  20. results show that IL-23 accounts for the main aspects of human and murine lupus including the expansion of double negative T cells, decreased IL-2, and increased IL-17 production PMID: 28646040
  21. studies revealed a new epigenetic pathway in the control of IL-2 production in systemic lupus erythematosus whereby low levels of miR-200a-3p accumulate the binding of the ZEB1-CtBP2 complex to the IL-2 promoter and suppress IL-2 production PMID: 28438897
  22. data suggest that the use of Golgi transport inhibitors results in an underestimation of the presence of type 2 cytokine-secreting cells and highlight IL-2 as a critical component in optimal cytokine production by Th2 and Th9 cells in vitro and in vivo PMID: 28468971
  23. These results suggested that TSC-22 could counteract the protective effect of GILZ on IL-2-deprivation-induced apoptosis. PMID: 26752201
  24. this study shows that IL-2 modulates the TCR signaling threshold for CD8 but not CD4 T cell Proliferation on a single-cell level PMID: 28159902
  25. These data demonstrate an important synergetic role of TGF-beta and IL-2 in the generation, activation and stability of Treg cells, as well as their subsequent development into follicular regulatory T cells. PMID: 27787514
  26. Mecamylamine, a nAChR inhibitor, suppressed not only these [Ca(2+)]i transients, but also IL-2 release and T cell proliferation PMID: 28025040
  27. Flavonoid glycosides of Alchornea floribunda did not result in detectable Il2 secretion by treated splenic T-lymphocytes. PMID: 26974045
  28. We also discuss the role of interleukin 2 (IL-2), which is decisive for the function of Treg and has been used therapeutically in preliminary trials in human SLE. The identification of novel Treg markers and the development of novel therapeutic approaches, which restore the balance between Treg and autoreactive Tcells are future goals for research in SLE. PMID: 26975190
  29. Data show that after tumor necrosis factor alpha-induced protein 8 like-2 (TIPE2) gene was down-regulated, the expression of the CD69 antigen was increased, and the proliferation of T lymphocytes and the secretion of cytokines IL-2 and IFN-gamma were enhanced. PMID: 27363266
  30. This study evaluated a chemical genetic toolkit that evaluated a biphasic requirement for JAK3 kinase activity in IL-2-driven T cell proliferation. PMID: 27018889
  31. Dominance of regulatory T cells in carcinogen-induced fibrosarcomas is not T-bet or Il-2 dependent. PMID: 26433463
  32. TCF1 is required for the T follicular helper cell response to viral infection functioning through negative feedback loops with IL-2 and Blimp1. PMID: 26365183
  33. These results may provide an additional understanding of the characteristics of the various fractions of isolated Tregs based on phenotype and function and the role of varying levels of exogenous IL-2 on the suppressive activity of these cells. PMID: 26529512
  34. findings demonstrate that distinct niches within the lymphoid organ T zone support distinct cell fate decisions, and they establish a function for dendritic-cell-derived CD25 in controlling IL-2 availability and T-cell differentiation PMID: 27147029
  35. IL-2 signaling modulates TH1 cell, follicular helper T cell and central memory T cell gene expression. PMID: 26743592
  36. Ndrg1 is a T-cell clonal anergy factor negatively regulated by IL-2. PMID: 26507712
  37. Innate cell-derived IL-2 is a critical cofactor in regulating innate lymphoid cell function in pulmonary type 2 pathology PMID: 26025126
  38. glucosamine interferes with N-glycosylation of CD25, and thereby attenuates IL-2 downstream signaling PMID: 26468284
  39. Interleukin-2 critically regulates bone marrow erythropoiesis and prevents anemia development. PMID: 26404745
  40. findings identified that autocrine IL-2 production operates in a dose-dependent fashion to facilitate the expansion potential of Ag-specific CD8(+) T cell populations, which may instigate ways to augment therapies depending on fit CD8(+) T cells. PMID: 26453748
  41. IL-2-mediated activation of the Akt kinase and mTORc1 signaling was both necessary and sufficient to shift differentiation away from Tfh cells, instead promoting that of Th1 cells. PMID: 26410627
  42. This study identified a novel long-range enhancer of the Il2 gene located 83 kb upstream of the transcription start site. PMID: 26351138
  43. The amelioration by Gl-PS against the suppression of the production of IL-2, IFN-gamma and TNF-alpha in mononuclear lymphocytes by B16F10 cell culture supernatant might contribute to cancer control. PMID: 25585987
  44. Late IL-2 promotes survival through acute downregulation of apoptotic pathways in effector T cells and by permanently upregulating their IL-7 receptor expression, enabling IL-7 to sustain them as memory T cells. PMID: 25369785
  45. enhances anti-CD45RBmAb-induced immune tolerance to skin allograft via up-regulated T regulatory cells PMID: 25550088
  46. Chronodependent effect of interleukin-2 on mouse spleen cells in the model of cyclophosphamide immunosuppression. PMID: 25708328
  47. Peripherally Induced Tolerance Depends on Peripheral Regulatory T Cells That Require Hopx To Inhibit Intrinsic IL-2 Expression. PMID: 26170384
  48. The morphologic changes and rapid cell death induced by dimeric IL-2 imply that cell death is mediated by disruption of membrane permeability and subsequent necrosis. PMID: 25019288
  49. autocrine IL-2 signaling is functional in GM-CSF myeloid dendritic cells in an early time window after PAMPs stimulation PMID: 25652593
  50. IL-2 produced by antigen-bearing dendritic cells (DCs) had a key role in Treg cell development and that existing Treg cells limited new development of Treg cells by competing for IL-2. PMID: 25939026
  51. this study has found no evidence for a direct role of IL-2 or Treg cells in negatively regulating dendritic cell number. PMID: 25590474
  52. In a condition of low inflammation, the Teff cell-mediated Treg cell boost involved TNF, OX40L, and plasmacytoid dendritic cells, whereas in a condition of high inflammation, it involved TNF and IL-2. PMID: 25548233
  53. Inflammation and lymphopenia trigger autoimmunity by suppression of IL-2-controlled regulatory T cell and increase of IL-21-mediated effector T cell expansion. PMID: 25339665
  54. Ab/IL-2 treatment was not sufficient to reverse hyperglycemia. PMID: 24205242
  55. In mice, low-dose IL-2-anti-IL-2 antibody complexes drove group 2 innate lymphoid cells (ILC2) to produce IL-5 and proliferate. PMID: 25323825
  56. inflammatory signals induced by viral infection may overcome the need for strong IL-2 signals in driving cytotoxicity in CD4 cells PMID: 24586481
  57. IL-2 increases both proliferation and death rates; the latter by downregulating Bcl2. Specifically, IL-2 increases the proliferation rate cooperatively and the death rate linearly; a relationship that, in the mathematical model, creates the bistable dynamics needed to provide both homeostasis and an OFF-state. PMID: 25171404
  58. findings indicate a general role for CD25 in contexts where IL-2 signaling is not involved, and may have important implications for all mast cell-related diseases, as well as in all cell types expressing CD25 independently of its IL-2-related functions. PMID: 25063866
  59. data suggest that administration of IL-2 to lupus-prone mice protects against end-organ damage and suppresses inflammation by dually limiting IL-17-producing DN T cells and expanding Treg. PMID: 25063876
  60. The induction of Foxp3 in precursor gammadelta T cells suggested that IL-2 could activate the Foxp3 gene early in thymocyte development. PMID: 25218473
  61. IL-2 induction of Blimp-1 expression is a key regulator of SLEC differentiation in vivo. PMID: 25015830
  62. Collectively, our results suggest that up-regulation of Tet2 is required for Foxp3 stability and IL2 is required to maintain the high level of Tet2 during the thymic Treg development. PMID: 24984151
  63. Tregs promote influenza-specific B-cell responses by preventing excessive IL-2 signalling, which suppresses Tfh cell differentiation. PMID: 24633065
  64. regulatory T cell exert a check on expansion and effector differentiation of CD8+ T cells under strongly immunogenic conditions associated with TLR ligand activation of dendritic cells, and this is mediated by limiting IL-2 availability. PMID: 24454872
  65. Our results suggest that CD4 positive, IL-2, and IL-4 participate in the HSV-1-induced facial paralysis immune response. PMID: 24281729
  66. Under IL-2 deprivation conditions, IL-21 may act as the major survival factor promoting T cell immune responses. PMID: 24416451
  67. this study describes a 5-methylcytosine glycosylase activity for the murine DNA base excision repair enzyme Myh and show that it is critically involved in remodeling the IL-2 Promoter for transcription. PMID: 24291244
  68. by repressing autocrine IL-2 production, Ikaros ensures that naive CD8(+) T cells remain dependent on licensing by APCs and CD4(+) T cells, and it may therefore act as a cell-intrinsic safeguard against inappropriate CTL differentiation PMID: 24778448
  69. Opposing actions of IL-2 and IL-21 on Th9 differentiation correlate with their differential regulation of BCL6 expression. PMID: 24550509
  70. Data strongly suggest that Tregs compete with T cells for IL-2. PMID: 24117537
  71. Cyclosporine A attenuated antigen-specific Tconv proliferation but permitted IL-2-induced regulatory T cell expansion. PMID: 23834842
  72. IL-2-mediated extracellular positive & negative feedback circuits are coupled & become activated at a similar level of IL-2 signaling. Secreted IL-2 drove high IL-2Ralpha expression, reduced IL-2 synthesis, & cell proliferation. PMID: 24244020
  73. These negative signaling events result in an anergic phenotype in which the T cells are not competent to signal through the IL-2 receptor. PMID: 23895744
  74. IL-2 acts as a self-regulatory circuit integrating the homeostasis of activated and T reg cells as CD4(+) T cells restrain their growth by monitoring IL-2 levels, thereby preventing uncontrolled responses and autoimmunity. PMID: 24249704
  75. Ets-1 promotes the expression of IL-2 by modulating the activity of NFAT. PMID: 24019486
  76. The IL-2/anti-IL-2 antigen-antibody complex("IL-2C") is a mediator of regulatory T (Treg) cell expansion and can attenuate renal ischemia-reperfusion injury and renal fibrosis in mice. PMID: 23833258
  77. Therefore, LDR, in the presence of suboptimal cytokine levels, can facilitate anti-tumor cytotoxicity of NK cells without influencing cellular proliferation or apoptosis. PMID: 22915781
  78. CD127(+) NK cells expanded in an IL-2-dependent manner. PMID: 23650439
  79. T reg cells regulate NK cell functions by controlling the bioavailability of limiting amounts of IL-2 in the islets. PMID: 23650440
  80. IL-2 rapidly boosted the capacity of NK cells to productively engage target cells. PMID: 23650441
  81. Cell-intrinsic IL-2 signaling is critical for Th1 development but plays a limited role in Th17 development in vitro as well as in vivo. PMID: 23715742
  82. IL-2 pretreatment expanded Treg cells while preventing the induction of Th17 during EAE development. PMID: 22954711
  83. brain-derived IL-2 plays an essential role in the maintainance of septohippocampal projection neurons in vivo. PMID: 23416322
  84. the expansion and accumulation of the Vbeta5(+) Tregs was IL-2 independent but dependent on TNF-alpha. These experiments reveal a subset-specific Treg induction by a new pathway. PMID: 23645880
  85. Decreased IL-2 production impairs the regulatory capacity of memory-like autoregulatory CD8(+) CD122(+) T cells. PMID: 23180662
  86. Interleukin-2 signalling is modulated by a labile disulfide bond in the CD132 chain of its receptor. PMID: 22645657
  87. Il2 and Csf2 gene expression, mediated by NFkappaB, are increased as T cells undergo activation and aging. PMID: 23079711
  88. In skin transplantation model exogenous IL-2 allowed prolonged allograft survival only in the case of a limited CD4 T cell-mediated alloreactivity. PMID: 23146537
  89. pRS-mIL-2 exhibited strong antitumour efficacy following consecutive induction with RU486. PMID: 22999060
  90. IL-2 signaling of CD4+ T cells play a critical role in multiple phases of CD8+ cytotoxic T lymphocytes responses following adenovirus vaccination. PMID: 23071696
  91. Nuclear activation of c-Rel regulates the initiation of GM-CSF and IL-2 gene activation in response to T cell activation and the termination of these gene responses following the removal of the activating signal. PMID: 22860011
  92. Endogenous IL-2 produced by CD8+ immune T cells can play an important autocrine-enhancing role on their IFN-gamma production in the secondary responses to T. gondii. PMID: 23359502
  93. Poly-G oligonucleotides directly induce the phosphorylation of Lck (an essential element of the T cell-signaling pathway), thereby enhancing production of IL-2, CD8 T cell proliferation, and antitumor activity. PMID: 23296706
  94. Higher levels of IL-2 in the tumour microenvironment eliminated the progressive growth of the B16 cells in vivo, and this inhibition was dependent on the presence of either T cells or, to a lesser extent, natural killer cells. PMID: 23198850
  95. Helios regulates IL-2 production in Tregs by suppressing Il2 gene transcription. Loss of Helios in Tregs breaks their anergic phenotype and results in derepression of the Il2 locus. PMID: 23275607
  96. The high level of IL-2 mRNA during early stage of infection was associated with clearance of mucosal Candidia albicans. PMID: 18704316
  97. Anergy-induced deacetylation of the Il2 promoter permits binding of the histone methyl-transferase Suv39H1. PMID: 22684523
  98. IL-2 treatment restores tumor growth and regulatory (Treg) cell generation in MIF-deficient mice. PMID: 22972922
  99. IL-2 alone is sufficient to drive regulatory T cell homeostatic proliferation PMID: 22933631
  100. Absence of calcium/calmodulin-dependent protein kinase IV (CaMK4) restores IL-2 production, curbs increased T cell activation, and augments the number and activity of regulatory T cells in MRL/lpr lupus-prone mice. PMID: 22942433

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Subcellular Location Secreted
Protein Families IL-2 family
Database Links

KEGG: mmu:16183

STRING: 10090.ENSMUSP00000029275

UniGene: Mm.14190

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