Mouse Interleukin 2,IL-2 ELISA kit

Product Details

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Product Details

Associated names

Il2 ELISA kit; Il-2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit

Abbreviation

IL2

Uniprot No.

P04351

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates, cell culture supernates

Detection Range

3.9 pg/ml-250 pg/ml

Sensitivity

0.975 pg/ml

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-95
1:2	Average %	92
	Range %	85-99
1:4	Average %	98
	Range %	91-105
1:8	Average %	93
	Range %	86-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

250

2.278

2.234

2.256

2.104

125

1.443

1.467

1.455

1.303

62.5

0.904

0.888

0.896

0.744

31.25

0.473

0.499

0.486

0.334

15.6

0.341

0.321

0.331

0.179

7.8

0.245

0.253

0.249

0.097

3.9

0.208

0.198

0.203

0.051

0.149

0.155

0.152

Materials provided

A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse IL-2 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled IL-2 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

Materials not provided

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This kit provides quantitative measurement of interleukin-2 (IL-2) in mouse samples, supporting research into T cell activation, proliferation, and immune regulation. Because IL-2 is produced transiently and at low concentrations following T cell receptor engagement, detecting it requires an assay calibrated for the picogram-level abundances typical of both *in vivo* and *in vitro* immunological models.

Detection Range: The 3.9–250 pg/ml range spans the concentrations expected in mouse serum during acute immune responses and in supernatants from antigen- or mitogen-stimulated splenocyte cultures. Most resting or mildly stimulated samples fall within range without dilution, while strongly activated cultures may require a simple pre-dilution step.

Sensitivity: At 0.975 pg/ml, the assay detects IL-2 in conditions where secretion is minimal — such as early time points post-stimulation, regulatory T cell co-cultures that suppress IL-2 production, or serum from naïve mice — where concentrations often sit in the low single-digit pg/ml range.

Sample Compatibility: Validation across serum, plasma, tissue homogenates, and cell culture supernatants allows researchers to correlate systemic IL-2 levels with local tissue microenvironment concentrations and *in vitro* functional readouts within a single experimental framework.

Assay Efficiency: A 50–100 µl sample requirement accommodates the limited volumes obtainable from mouse orbital bleeds or primary T cell cultures, while the 1–5 hour protocol fits within same-day workflows alongside proliferation or cytotoxicity assays.

This kit is well suited for studies of graft-versus-host disease, checkpoint immunotherapy response, and Treg-mediated immune suppression in mouse models.

Code	CSB-E04627m-IS
Size	96T,5×96T,10×96T
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.