Mouse Interleukin 2,IL-2 ELISA kit

Code CSB-E04627m
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P04351
Lead Time 3-5 working days
Abbreviation IL2
Protein Biological Process 1 Immunity
Target Name interleukin 2
Alias IL-2, TCGF, lymphokine, T cell growth factor|aldesleukin|interleukin-2|involved in regulation of T-cell clonal expansion
Species Mus musculus (Mouse)
Protein Biological Process 3 Adaptive immunity
Sample Types serum, plasma, tissue homogenates, cell culture supernates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %81-93
1:2Average %99
Range %93-105
1:4Average %95
Range %90-100
1:8Average %96
Range %90-104
Recovery
The recovery of mouse IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9388-98
EDTA plasma (n=4)9790-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10001.729 1.652 1.691 1.597
5001.434 1.386 1.410 1.316
2501.091 1.004 1.048 0.954
1250.765 0.729 0.747 0.653
62.50.428 0.421 0.425 0.331
31.20.276 0.269 0.273 0.179
15.60.193 0.179 0.186 0.092
00.097 0.090 0.094  
References
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Function Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Subcellular Location Secreted
Protein Families IL-2 family
Database Links

KEGG: mmu:16183

STRING: 10090.ENSMUSP00000029275

UniGene: Mm.14190

Pathway Jak-STAT signaling pathway
PI3K-Akt signaling pathway
T cell receptor signaling pathway
Th1 and Th2 cell differentiation
Th17 cell differentiation

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