Mouse Interleukin 2,IL-2 ELISA kit

Code CSB-E04627m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


The Mouse Interleukin 2 (IL-2) ELISA kit is designed for detection and quantification of human IL-2 in serum, plasma, tissue homogenates, or cell culture supernates. It has been validated with high sensitivity, strong specificity, good linearity, precision less than 10%, high recovery, and consistency across batches. Refer to the product instructions for more details.

The measurement of this assay is based on the Sandwich-ELISA technique. The samples and biotin-labeled IL-2 antibody are successively pipetted into the wells pre-coated with anti-mouse IL-2 antibody. The IL-2 is sandwiched between the two antibodies, forming Ag-Ab-Ag immune complex. HRP-avidin is subsequently added to the wells. After thorough washing, the substrate TMB is added for color development. TMB is converted to blue by HRP and yellow by the stop solution. The intensity of the color is positively correlated with the IL-2 content in the samples.

IL-2 is a pleiotropic cytokine produced by activated T cells. It is involved in various biological processes, including the differentiation and survival of CD4+ T helper subsets and CD4+ T regulatory cells, promotion of the cytolytic activity of natural killer (NK) cells and T cells, and augment of immunoglobulin production via activated B cells. IL-2 controls diverse T cell biology by binding with IL-2R. And IL-2/IL-2R signaling is important for T cell activation through the JAK1-STAT5 signaling pathway.

Target Name interleukin 2
Alternative Names Il2 ELISA kit; Il-2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
Abbreviation IL2
Uniprot No. P04351
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell culture supernates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %87
Range %81-93
1:2Average %99
Range %93-105
1:4Average %95
Range %90-100
1:8Average %96
Range %90-104
The recovery of mouse IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9388-98
EDTA plasma (n=4)9790-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
10001.729 1.652 1.691 1.597
5001.434 1.386 1.410 1.316
2501.091 1.004 1.048 0.954
1250.765 0.729 0.747 0.653
62.50.428 0.421 0.425 0.331
31.20.276 0.269 0.273 0.179
15.60.193 0.179 0.186 0.092
00.097 0.090 0.094  
Materials provided
  • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse IL-2 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled IL-2 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


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Target Background

(From Uniprot)
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Gene References into Functions
  1. These studies demonstrate that Th1 CD4(+) T cells require IL-2 for lung T resident memory cell development. PMID: 28948612
  2. Because IL-2 production was limited to cells receiving the strongest T cell receptor (TCR) signals, a direct link between TCR-signal strength, IL-2 production, and T cell fate determination has been established. PMID: 30213884
  3. These data highlights the existence of IL-2 trans-presentation between NK cells in the local microenvironment where the availability of IL-2 is limited. PMID: 28074895
  4. interleukin-2 (IL-2) is a non-pancreatic autoimmune target in type 1 diabetes PMID: 27708334
  5. Each mutation decreased STAT5 binding and altered IL-2-induced Il2ra gene expression, revealing that individual elements within the superenhancer were not functionally redundant and that all were required for normal gene expression. PMID: 29078395
  6. Deleting IL-2 in CD11c(high)MHCII(+) cells induces spontaneous colitis resembling human inflammatory bowel disease. PMID: 29549257
  7. a significant increase in plasma levels of IL-2, IFN-g and TNF-g was revealed as assessed by ELISA. In conclusion, the results of the present study indicate that MENK has a cytotoxic effect on B16 melanoma cells in vitro and in vivo, and suggest a potential mechanism for these bioactivities. PMID: 28849104
  8. WASp knockout mice controlled growth of A20 lymphoma cells that naturally produced IL-2. PMID: 27477778
  9. Tumor growth delays observed by tumor irradiation combined with MVA-MUC1-IL-2 vaccine were significantly more prolonged than those observed by vaccine, radiation, or radiation with MVA empty vector. PMID: 28116088
  10. IL-2 signalling is essential to prevent deletion of CD4SP CCR7+ Helios+ thymocytes at a later developmental stage than Card11 is required to prevent deletion. The deletion prevented by IL-2 signalling occurs in a Foxp3-independent manner. PMID: 28362433
  11. in vivo targeting of the TNF superfamily receptor TNFRSF25 using the TL1A-Ig fusion protein, along with IL-2, resulted in transient but massive Treg expansion in donor mice; transplantation of Treg-expanded donor cells facilitated transplant tolerance without GVHD, with complete sparing of graft-versus-malignancy. PMID: 28219835
  12. IL-2 and IL-6 work together to enhance influenza-specific CD8 T cell generation responding to live influenza virus in aged mice and humans PMID: 27322555
  13. this report, we elucidated the unsolved mechanism of the anti-cancer effect of curcumin by identifying IL-2 as a direct molecular target. Curcumin, as a small molecule IL-2 modulator, has the potential to be used to treat IL-2 related pathologic conditions. PMID: 29127008
  14. Data show that PTEN plays a key role in Th17 cell differentiation by blocking IL-2 expression. PMID: 29018045
  15. AnxA6 regulates IL-2 homeostasis and sensitivity in T cells by sustaining a lipid raft-like membrane environment. PMID: 26853809
  16. NOD Treg cells have an impaired responsiveness to IL-2 that reduces their ability to compete for a limited supply of IL-2. PMID: 26763864
  17. Suppression of IL-12p70 formation by IL-2 or following macrophage depletion causes T-cell autoreactivity leading to CNS demyelination in HSV-1-infected mice. PMID: 28542613
  18. At single cell level, IL-2 is binary (digital) and CD25 is graded expressed, whereas at population level both parameters show graded expression correlating with the antigen amount. PMID: 28035902
  19. IL-2/anti-IL-2 complexes protected lupus-prone mice against lupus nephritis by expanding Tregs. PMID: 27914701
  20. results show that IL-23 accounts for the main aspects of human and murine lupus including the expansion of double negative T cells, decreased IL-2, and increased IL-17 production PMID: 28646040
  21. studies revealed a new epigenetic pathway in the control of IL-2 production in systemic lupus erythematosus whereby low levels of miR-200a-3p accumulate the binding of the ZEB1-CtBP2 complex to the IL-2 promoter and suppress IL-2 production PMID: 28438897
  22. data suggest that the use of Golgi transport inhibitors results in an underestimation of the presence of type 2 cytokine-secreting cells and highlight IL-2 as a critical component in optimal cytokine production by Th2 and Th9 cells in vitro and in vivo PMID: 28468971
  23. These results suggested that TSC-22 could counteract the protective effect of GILZ on IL-2-deprivation-induced apoptosis. PMID: 26752201
  24. this study shows that IL-2 modulates the TCR signaling threshold for CD8 but not CD4 T cell Proliferation on a single-cell level PMID: 28159902
  25. These data demonstrate an important synergetic role of TGF-beta and IL-2 in the generation, activation and stability of Treg cells, as well as their subsequent development into follicular regulatory T cells. PMID: 27787514
  26. Mecamylamine, a nAChR inhibitor, suppressed not only these [Ca(2+)]i transients, but also IL-2 release and T cell proliferation PMID: 28025040
  27. Flavonoid glycosides of Alchornea floribunda did not result in detectable Il2 secretion by treated splenic T-lymphocytes. PMID: 26974045
  28. We also discuss the role of interleukin 2 (IL-2), which is decisive for the function of Treg and has been used therapeutically in preliminary trials in human SLE. The identification of novel Treg markers and the development of novel therapeutic approaches, which restore the balance between Treg and autoreactive Tcells are future goals for research in SLE. PMID: 26975190
  29. Data show that after tumor necrosis factor alpha-induced protein 8 like-2 (TIPE2) gene was down-regulated, the expression of the CD69 antigen was increased, and the proliferation of T lymphocytes and the secretion of cytokines IL-2 and IFN-gamma were enhanced. PMID: 27363266
  30. This study evaluated a chemical genetic toolkit that evaluated a biphasic requirement for JAK3 kinase activity in IL-2-driven T cell proliferation. PMID: 27018889
  31. Dominance of regulatory T cells in carcinogen-induced fibrosarcomas is not T-bet or Il-2 dependent. PMID: 26433463
  32. TCF1 is required for the T follicular helper cell response to viral infection functioning through negative feedback loops with IL-2 and Blimp1. PMID: 26365183
  33. These results may provide an additional understanding of the characteristics of the various fractions of isolated Tregs based on phenotype and function and the role of varying levels of exogenous IL-2 on the suppressive activity of these cells. PMID: 26529512
  34. findings demonstrate that distinct niches within the lymphoid organ T zone support distinct cell fate decisions, and they establish a function for dendritic-cell-derived CD25 in controlling IL-2 availability and T-cell differentiation PMID: 27147029
  35. IL-2 signaling modulates TH1 cell, follicular helper T cell and central memory T cell gene expression. PMID: 26743592
  36. Ndrg1 is a T-cell clonal anergy factor negatively regulated by IL-2. PMID: 26507712
  37. Innate cell-derived IL-2 is a critical cofactor in regulating innate lymphoid cell function in pulmonary type 2 pathology PMID: 26025126
  38. glucosamine interferes with N-glycosylation of CD25, and thereby attenuates IL-2 downstream signaling PMID: 26468284
  39. Interleukin-2 critically regulates bone marrow erythropoiesis and prevents anemia development. PMID: 26404745
  40. findings identified that autocrine IL-2 production operates in a dose-dependent fashion to facilitate the expansion potential of Ag-specific CD8(+) T cell populations, which may instigate ways to augment therapies depending on fit CD8(+) T cells. PMID: 26453748
  41. IL-2-mediated activation of the Akt kinase and mTORc1 signaling was both necessary and sufficient to shift differentiation away from Tfh cells, instead promoting that of Th1 cells. PMID: 26410627
  42. This study identified a novel long-range enhancer of the Il2 gene located 83 kb upstream of the transcription start site. PMID: 26351138
  43. The amelioration by Gl-PS against the suppression of the production of IL-2, IFN-gamma and TNF-alpha in mononuclear lymphocytes by B16F10 cell culture supernatant might contribute to cancer control. PMID: 25585987
  44. Late IL-2 promotes survival through acute downregulation of apoptotic pathways in effector T cells and by permanently upregulating their IL-7 receptor expression, enabling IL-7 to sustain them as memory T cells. PMID: 25369785
  45. enhances anti-CD45RBmAb-induced immune tolerance to skin allograft via up-regulated T regulatory cells PMID: 25550088
  46. Chronodependent effect of interleukin-2 on mouse spleen cells in the model of cyclophosphamide immunosuppression. PMID: 25708328
  47. Peripherally Induced Tolerance Depends on Peripheral Regulatory T Cells That Require Hopx To Inhibit Intrinsic IL-2 Expression. PMID: 26170384
  48. The morphologic changes and rapid cell death induced by dimeric IL-2 imply that cell death is mediated by disruption of membrane permeability and subsequent necrosis. PMID: 25019288
  49. autocrine IL-2 signaling is functional in GM-CSF myeloid dendritic cells in an early time window after PAMPs stimulation PMID: 25652593
  50. IL-2 produced by antigen-bearing dendritic cells (DCs) had a key role in Treg cell development and that existing Treg cells limited new development of Treg cells by competing for IL-2. PMID: 25939026

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Subcellular Location Secreted
Protein Families IL-2 family
Database Links

KEGG: mmu:16183

STRING: 10090.ENSMUSP00000029275

UniGene: Mm.14190

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