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| Code | CSB-AP001981MOW |
| Size | $3140 |
| Image |
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| Have Questions? | Leave a Message or Start an on-line Chat |
| Purity | >98% as determined by SDS-PAGE and HPLC. |
| Endotoxin | Less than 1.0 EU/μg as determined by LAL method. |
| Activity | Assay #1: Fully biologically active when compared to standard. The ED50 as determined by the dose- dependent stimulation of the proliferation of murine NFS-60 cells is less than 5.0 ng/ml, corresponding to a specific activity of >2x105 IU/mg. Assay #2: The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.05 ng/ml, corresponding to a specific activity of >2.0x107 IU/mg. |
| Target Names | IL3 |
| Uniprot No. | P25140 |
| Research Area | Immunology |
| Alternative Names |
IL3; Interleukin-3; IL-3; Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor
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| Species | Macaca mulatta (Rhesus macaque) |
| Source | E.coli |
| Expression Region | 20-143aa |
| Complete Sequence | APMTQTTSLK TSWAKCSNMI DEIITHLNQP PLPSPDFNNL NEEDQTILVE KNLRRSNLEA FSKAVKSLQN ASAIESILKN LPPCLPMATA APTRPPIRIT NGDRNDFRRK LKFYLKTLEN EQAQ |
| Mol. Weight | 14.0 kDa |
| Protein Length | Full Length of Mature Protein |
| Tag Info |
Tag-Free |
| Form | Lyophilized powder |
| Buffer | Lyophilized from a 0.2 µm filtered PBS, pH 7.4, 5 % trehalose |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs |
Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | 5-10 business days |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |
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| Function |
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.; This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
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| Subcellular Location | Secreted. |
| Protein Families | IL-3 family |
| Tissue Specificity | Activated T-cells, mast cells, natural killer cells. |
| Database Links |
KEGG: mcc:706946 STRING: 9544.ENSMMUP00000022233 UniGene: Mmu.16387 |
