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Western Blot Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Mouse spleen tissue, Rat spleen tissue, Mouse heart tissue, Rat heart tissue All lanes HSPA8 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 10s
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Western Blot Positive WB detected in: Hela whole cell lysate at 20μg, 10μg, 5μg, 2.5μg, 1.25μg, 0.625μg All lanes: HSPA8 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 10s
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Western Blot Positive WB detected in: 20μg Hela whole cell lysate HSPA8 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000, 1:256000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 10s
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IHC image of CSB-MA010829A0m diluted at 1:256 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight, and detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
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IHC image of CSB-MA010829A0m diluted at 1:256 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight, and detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Immunoprecipitating HSPA8 in Hela whole cell lysate Lane 1: Mouse control IgG2b instead of CSB-MA010829A0m in Hela whole cell lysate Lane 2: CSB-MA010829A0m (1.5µl) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg) For western blotting, the blot was detected with CSB-MA010829A0m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000 .
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Overlay histogram showing MCF-7 cells stained with CSB-MA010829A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the primary antibody at 1:200 for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b used under the same conditions. Acquisition of >10,000 events was performed.
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