Recombinant Bovine Fibroblast growth factor 2 (FGF2) (Active)

FGF2 orchestrates cell proliferation, survival, and differentiation across multiple lineages, making it a critical tool for dissecting mitogenic signaling and tissue regeneration pathways. This tag-free bovine ortholog, spanning the complete mature sequence (aa 10–155), demonstrates robust bioactivity with an ED50 ≤2.0 ng/mL in NIH3T3 fibroblast proliferation assays, confirming potent receptor engagement suitable for dose-response studies in stem cell expansion, neuronal differentiation protocols, and angiogenesis models. Expressed in *E. coli* to yield an unglycosylated form that retains full mitogenic function, the protein meets the purity and endotoxin criteria (>95% by SDS-PAGE; ≤200 EU/mg) typical for cell-based assays and receptor-ligand binding experiments, including ELISA and surface plasmon resonance. Researchers investigating FGF receptor specificity, wound healing mechanisms, or osteogenic differentiation will find this preparation provides a quantitatively validated basis for mechanistic and functional studies.