Recombinant Human/Cynomolgus Activin receptor type-2A (ACVR2A), partial (Active)

In Stock
Code CSB-MP001260HU1
MSDS
Size $110
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized Human ACVR2A at 2 μg/ml can bind Anti-ACVR2A&ACVR2B recombinant antibody (CSB-RA623829MA1HU). The EC50 is 3.848-4.375 ng/mL. Biological Activity Assay
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Product Details

Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human ACVR2A at 2 μg/mL can bind Anti-ACVR2A recombinant antibody (CSB-RA623829MA1HU). The EC50 is 3.848-4.375 ng/mL.
Target Names
Uniprot No.
Alternative Names
Activin receptor type-2A; EC:2.7.11.30; Activin receptor type IIA (ACTR-IIA; ACTRIIA);ACVR2A; ACVR2
Species
Homo sapiens (Human)
Source
Mammalian cell
Expression Region
20-135aa
Target Protein Sequence
AILGRSETQECLFFNANWEKDRTNQTGVEPCYGDKDKRRHCFATWKNISGSIEIVKQGCWLDDINCYDRTDCVEKKDSPEVYFCCCEGNMCNEKFSYFPEMEVTQPTSNPVTPKPP
Mol. Weight
14.8kDa
Protein Length
Partial
Tag Info
C-terminal 10xHis-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

A DNA fragment encoding the 20-135aa of human ACVR2A was fused with the 10xHis-tag at the C-terminus and expressed in mammalian cells through a vector. The resulting product is the recombinant human ACVR2A protein. It carries a C-terminal 10xHis-tag. Its purity exceeds 95% as determined by SDS-PAGE. It has been validated to be an active protein in a functional ELISA, where immobilized human ACVR2A at 2 μg/mL can bind the anti-ACVR2A recombinant antibody (CSB-RA623829MA1HU), with the EC50 of 3.848-4.375 ng/mL.

The ACVR2A protein is a multifunctional receptor that integrates signals from the TGF-β superfamily to regulate a wide array of biological functions, including muscle growth, immune responses, and skeletal development. It is integral to signaling pathways that regulate cellular proliferation, differentiation, and apoptosis, thereby influencing tissue homeostasis and development [1][2].

ACVR2A mainly regulates muscle growth and differentiation. It is activated by activins, myostatin, and BMPs, which are crucial for muscle development and maintenance [2]. Furthermore, ACVR2A signaling has been implicated in the pathophysiology of various diseases, including cancer and fibrosis. ACVR2A has been shown to mediate the effects of activin A in promoting fibrotic processes in liver diseases, particularly through its interaction with hepatic stellate cells [3]. This highlights its role in inflammation and tissue remodeling, which are critical in chronic disease states.

ACVR2A also modulates immune responses. It is involved in the differentiation of T-helper cells, particularly Th17 cells, which are known to contribute to autoimmune conditions [3]. The signaling pathways activated by ACVR2A can lead to both pro-inflammatory and anti-inflammatory responses, depending on the context and the ligands involved [4].

Moreover, ACVR2A is essential for proper skeletal development and homeostasis. It mediates signaling pathways that are crucial for bone formation and remodeling, and its dysregulation can lead to skeletal disorders [5]. Its involvement in both normal physiological processes and pathological conditions makes it a significant focus of research for therapeutic interventions in diseases such as cancer, fibrosis, and autoimmune disorders.

References:
[1] A. Kulminski, L. He, I. Culminskaya, Y. Loika, Y. Kernogitski, K. Arbeev, et al. Pleiotropic associations of allelic variants in a 2q22 region with risks of major human diseases and mortality, Plos Genetics, vol. 12, no. 11, p. e1006314, 2016. https://doi.org/10.1371/journal.pgen.1006314
[2] T. Bhattacharya, R. Shukla, R. Chatterjee, & S. Bhanja. Comparative analysis of silencing expression of myostatin (mstn) and its two receptors (acvr2a and acvr2b) genes affecting growth traits in knock down chicken, Scientific Reports, vol. 9, no. 1, 2019. https://doi.org/10.1038/s41598-019-44217-z
[3] H. Zhou, B. Ju, Y. Nie, B. Song, Y. Xu, & P. Gao. Adenovirus‑mediated knockdown of activin a receptor type�2a attenuates immune‑induced hepatic fibrosis in mice and inhibits interleukin‑17‑induced activation of primary hepatic stellate cells, International Journal of Molecular Medicine, 2018. https://doi.org/10.3892/ijmm.2018.3600
[4] C. Li, Z. Xu, X. Fan, H. Chen, Q. Yu, S. Fang, et al. Microrna-21 mediates the protective effects of mesenchymal stem cells derived from ipscs to human bronchial epithelial cell injury under hypoxia, Cell Transplantation, vol. 27, no. 3, p. 571-583, 2018. https://doi.org/10.1177/0963689718767159
[5] Y. Totoki, A. Yoshida, F. Hosoda, H. Nakamura, N. Hama, K. Ogura, et al. Unique mutation portraits and frequent col2a1 gene alteration in chondrosarcoma, Genome Research, vol. 24, no. 9, p. 1411-1420, 2014. https://doi.org/10.1101/gr.160598.113

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