Product Details

Purity

≥ 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human NCR3LG1 at 2 μg/mL can bind Anti-NCR3LG1 recombinant antibody(CSB-RA737873MA1HU). The EC50 is 10.90-12.22 ng/mL.

Target Names

NCR3LG1

Uniprot No.

Q68D85

Alternative Names

Natural cytotoxicity triggering receptor 3 ligand 1; B7 homolog 6 (B7-H6); NCR3LG1; B7H6

Species

Homo sapiens (Human)

Source

Baculovirus

Expression Region

25-262aa

Target Protein Sequence

DLKVEMMAGGTQITPLNDNVTIFCNIFYSQPLNITSMGITWFWKSLTFDKEVKVFEFFGDHQEAFRPGAIVSPWRLKSGDASLRLPGIQLEEAGEYRCEVVVTPLKAQGTVQLEVVASPASRLLLDQVGMKENEDKYMCESSGFYPEAINITWEKQTQKFPHPIEISEDVITGPTIKNMDGTFNVTSCLKLNSSQEDPGTVYQCVVRHASLHTPLRSNFTLTAARHSLSETEKTDNFS

Mol. Weight

28.1 kDa

Protein Length

Partial

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

B7-H6 functions as a stress-induced ligand on tumor cells and infected cells, engaging the activating receptor NKp30 on natural killer cells to trigger cytotoxicity—a pathway central to NK cell-mediated immune surveillance. This recombinant protein, spanning residues 25–262 and expressed in baculovirus, demonstrates specific antibody binding in functional ELISA with an EC50 of 10.90–12.22 ng/mL, confirming that the extracellular domain retains conformational epitopes required for receptor-ligand interaction assays and therapeutic antibody epitope mapping. The quantified binding activity supports its use in competitive inhibition screens to identify blocking antibodies, in SPR or BLI experiments for affinity characterization of NKp30-targeting biologics, and as a positive control in NK cell checkpoint research. With purity exceeding 95% by SDS-PAGE and endotoxin below 1.0 EU/μg, this protein meets the quality thresholds commonly required for drug discovery assays screening small-molecule or biologic inhibitors of the NKp30–B7-H6 axis.

Target Background

Function

Triggers NCR3-dependent natural killer cell activation.

Gene References into Functions

B7-H6 was expressed in gliomas and correlated with glioma progression. Knockdown of B7-H6 inhibited glioma cell proliferation, colony formation, migration, and invasion via upregulation of E-cadherin and Bax, and downregulation of vimentin, N-cadherin, MMP-2, MMP-9 and survivin. PMID: 29679856
these studies find that soluble B7H6 is constitutively expressed during pregnancy PMID: 29055565
Data suggest that B7-H6 play an important role in the regulation of the biological behavior of glioma cells. PMID: 28415577
Data indicate a sequence of events driven by tumour-derived prostaglandin D2 (PGD2) associated with engagement of the natural cytotoxicity triggering receptor 3 (NKp30)-B7H6 antigen (B7H6) pathway leading to significant group 2 innate lymphoid cells (ILC2s) activation and expansion. PMID: 28928446
B7H6-derived peptides trigger TNF-alpha-dependent immunostimulatory activity of lymphocytic NK92-MI cells. PMID: 27216712
Study confirms that B7-H6 is widely expressed in B-cell lymphomas and demonstrates its important role in the pathogenesis and chemosensitivity of lymphoma. PMID: 26891663
our present data revealed that higher B7-H6 expression in ovarian cancer tissues was positively correlated with tumor metastasis and cancer progression PMID: 26464699
NKp30-B7-H6 interaction is a novel cell contact mechanism that mediates activation of Group 2 innate lymphoid cells and identifies a potential target for the development of novel therapeutics for atopic dermatitis and other atopic diseases. PMID: 26582946
study not only reveals the possibility that tumor therapeutics work as stress inducers to enhance tumor sensitivity to NK cell cytolysis but also suggests that B7-H6 could be a potential target for tumor therapy in the future PMID: 26472927
Our results suggest that NKP30-B7-H6 interaction can aggravate hepatocyte damage, probably through up-regulation of IL-32 expression in hepatitis B virus-related acute-on-chronic liver failure PMID: 26241657
the interaction between NKp30 and B7-H6 may contribute to the fate of neuroblastoma patients PMID: 25877893
Study suggests that B7-H6 has a limited value as a prognostic marker in non-small cell lung cancer patients. PMID: 25400778
this study identified ectodomain shedding of B7-H6 by the metalloproteases "a disintegrin and metalloproteases" (ADAM)-10 and ADAM-17 from the cell surface of tumor cells as a crucial mechanism of expression regulation of B7-H6. PMID: 24780758
B7-H6-positive carcinomas were significantly associated with a higher differentiation. PMID: 24242703
Allogeneic and xenogeneic anti-tumor effect of callithrix jacchus natural killer cells is dependent on NKp30 and B7-H6 interaction. PMID: 25001651
Data indicate that the ectodomain of NKp30 forms functional homo-oligomers that mediate high affinity binding to its corresponding cellular ligand B7-H6. PMID: 24275655
These findings reveal that B7-H6 is not only implicated in tumor immunosurveillance but also participates in the inflammatory response in infectious conditions. PMID: 23687088
B7-H6:7D8 represents the first Ab-based molecule stimulating NKp30-mediated NK cell cytotoxicity for therapeutic purposes PMID: 23066150
The NKp30-B7-H6 structure revealed that this NK cell activating complex is distinct from the CTLA4-B7 and PD-1-PD-L T cell inhibitory complexes in both overall organization and detailed atomic interactions that mediate binding and specificity. PMID: 21422170
DKFZp686O24166 was designated the gene B7-H6; identification as a tumor cell surface molecule that binds NKp30, a receptor which triggers antitumor NK cell cytotoxicity and cytokine secretion PMID: 19528259

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Tissue Specificity

Not detected in any normal tissue tested. Expressed at the surface of several tumor cell lines including T and B-lymphomas, myeloid leukemias, melanomas, carcinomas and large T SV40 antigen-transformed cells (at protein level).

Database Links

HGNC: 42400

OMIM: 613714

KEGG: hsa:374383

STRING: 9606.ENSP00000341637

UniGene: Hs.146274

Code	CSB-BP737873HU
Abbreviation	Recombinant Human NCR3LG1 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$400
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Human NCR3LG1 at 2 μg/ml can bind Anti-NCR3LG1 recombinant antibody(CSB-RA737873MA1HU). The EC₅₀ is 10.90-12.22 ng/mL. Biological Activity Assay
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Recombinant Human Natural cytotoxicity triggering receptor 3 ligand 1 (NCR3LG1), partial (Active)

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