Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized human VEGF165 (CSB-MP025833HU(F4)) at 2 μg/ml can bind human NRP1, the EC50 is 22.68-34.55 ng/ml.
Research Area
Cardiovascular
Alternative Names
(Vascular endothelial cell growth factor 165 receptor)(CD304)
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
22-644aa
Target Protein Sequence
FRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEWLIQAPDPYQRIMINFNPHFDLEDRDCKYDYVEVFDGENENGHFRGKFCGKIAPPPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSPGFPEKYPNSLECTYIVFVPKMSEIILEFESFDLEPDSNPPGGMFCRYDRLEIWDGFPDVGPHIGRYCGQKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQSSVSEDFKCMEALGMESGEIHSDQITASSQYSTNWSAERSRLNYPENGWTPGEDSYREWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYKIDVSSNGEDWITIKEGNKPVLFQGNTNPTDVVVAVFPKPLITRFVRIKPATWETGISMRFEVYGCKITDYPCSGMLGMVSGLISDSQITSSNQGDRNWMPENIRLVTSRSGWALPPAPHSYINEWLQIDLGEEKIVRGIIIQGGKHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPTTPNGNLVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTVIDSTIQSGIK
Protein Length
Full Length of Mature Protein of Isoform 2
Tag Info
C-terminal hFc-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The gene fragment coding for amino acids 22-644 of the human Neuropilin-1 (NRP1) isoform 2 is expressed in mammalian cells with a human Fc tag at the C-terminus. The product is the recombinant human NRP1 protein. This NRP1 protein has been demonstrated to be active. In the functional ELISA, immobilized human VEGF165 can bind to this NRP1 protein. The EC50 is 22.68-34.55 ng/ml. It reaches up to 95% in purity. In the SDS-PAGE, this protein migrated to the band of molecular weight about 116 kDa on the gel. Its endotoxin is less than 1.0 EU/ug determined by the LAL method. This recombinant NRP1 protein is in stock.
NRP1, a member of a family of signaling and catalytic proteins, exists as two isoforms: a secreted form (sNRP1) and a transmembrane form. sNRP1 has been known to suppress cell-associated NRP-1 function in cervical cancer. And patients with cervical cancer have significantly higher circulating sNRP-1 levels. The transmembrane form of NRP1 plays important roles in the development of the nervous and cardiovascular system, tumorgenesis, and viral entry through interaction with VEGF, Sema3A, TGF-β, plexins, and integrins. Studies have demonstrated that NRP1 facilitates SARS-CoV-2 entry into host cells and significantly potentiates SARS-CoV-2 infectivity in vitro. NRP1 thus may potentially provide a therapeutic target for COVID-19.