Product Details

Uniprot No.

Target Names

Alternative Names

A5 protein antibody; BDCA4 antibody; BLOOD DENDRITIC CELL ANTIGEN 4 antibody; CD304 antibody; Neuropilin-1 antibody; Neuropilin1 antibody; NP1 antibody; NPN1 antibody; NRP 1 antibody; NRP antibody; NRP1 antibody; NRP1_HUMAN antibody; transmembrane receptor antibody; Vascular endothelial cell growth factor 165 receptor antibody; VEGF165R antibody

Species Reactivity

Human

Immunogen

Recombinant Human NRP1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

10C10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, FC

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Neuropilin-1 (NRP1) serves as a multifunctional transmembrane receptor with far-reaching implications across neuroscience, cardiovascular biology, and immunology research. As a co-receptor for both semaphorins and vascular endothelial growth factor (VEGF165), NRP1 plays critical roles in axonal guidance, angiogenesis, and immune cell function. Its expression on blood dendritic cells, where it is also known as BDCA4 or CD304, makes it a valuable marker for studying dendritic cell populations and their contributions to immune responses.

This recombinant monoclonal antibody, clone 10C10, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than hybridoma-derived sources, researchers benefit from lot-to-lot uniformity that supports longitudinal studies and ensures comparable results across experiments. The human IgG1 isotype format provides predictable behavior in detection systems while the affinity-chromatography purification yields a preparation suitable for sensitive applications.

Validation studies confirm robust performance in flow cytometry applications, with recommended dilutions ranging from 1:50 to 1:200. Testing in PC3 cells demonstrated clear positive signal separation from isotype control, with acquisition of over 10,000 events confirming reliable detection of intracellular NRP1 following fixation and permeabilization. The antibody is also validated for ELISA, offering flexibility for researchers working across different experimental platforms.

Whether investigating VEGF signaling in tumor angiogenesis, characterizing dendritic cell subsets, or exploring semaphorin-mediated neuronal development, this antibody provides a dependable tool for detecting human NRP1 in your research applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins. Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage. It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB. Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR.; Binds VEGF-165 and may inhibit its binding to cells. May induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.; (Microbial infection) Acts as a host factor for human coronavirus SARS-CoV-2 infection. Recognizes and binds to CendR motif RRAR on SARS-CoV-2 spike protein S1 which enhances SARS-CoV-2 infection.

Gene References into Functions

HLA-G, NRP1, and PD-1, may be involved in the immune response in psoriatic patients PMID: 29790686
miR-320/NRP-1 axis contributes to the growth and metastasis of cholangiocarcinoma cells. PMID: 28618167
we demonstrated that HS3ST3B enhances the proliferation and survival of breast cancer MDA-MB-231 cells, via a mechanism that is dependent on Nrp1. PMID: 30360368
The serum NRP1 was significantly associated with AFP, gamma-GT, Alb, bile acid, ALT, AST, ALP and pre-Alb. NRP1 is a novel tumor marker in HCC. PMID: 29966621
lnc00152 was aberrantly overexpressed in colorectal tumors and cancer cells and that lnc00152 was modulated by miRNA206. lnc00152 overexpression promoted epithelial-mesenchymal transition and increased neuropilin1 expression in the colorectal cancer cells. PMID: 29956750
the results presented herein indicate that NRP1 exerts tumor suppressive effects in NB, at least in part by regulating the expression of beta1 integrin PMID: 29750423
NRP1 may be important in the etiology of menstrual migraine. PMID: 29671086
clinical samples from apical periodontitis patients were obtained to analyse the expression of Sema3A/Nrp1. These results indicated that the bone destruction level expanded from days 7 to 35 PMID: 29457037
Study demonstrated that NRP1+ cells possess tumor-initiating cells (TIC) properties, including the preferential expression of stemness markers, higher clonogenic and self-renewal potential, and increased cell migration capacity. These findings support an important role for NRP1 in the biology of lung cancer TICs. PMID: 29138851
Authors demonstrated that when VEGFR2 was inhibited, NRP-1 appeared to regulate RAD51 expression through the VEGFR2-independent ABL-1 pathway, consequently regulating radiation sensitivity. In addition, the combined inhibition of VEGFR2 and NRP-1 appears to sensitize cancer cells to radiation. PMID: 29777301
NRP1, as a promoter of odontoblast differentiation, regulates dental pulp stem cells via the classical Wnt/beta-catenin pathway. PMID: 28910136
The new observation that shows strong NP-1 immunoreactivity of these particles, and decreased NP1 expression in syncytiotrophoblast of preeclampsia (PET) placentas in comparison to the control group, may imply a role of NP-1 in PET. PMID: 26937865
rs2228638 of NRP1 may have a role in tetralogy of Fallot risk in European and Chinese Han populations PMID: 29432830
Data show that neuropilin 1 (NRP1) binds extracellular AGO2 (carrying miRNA or not), and internalizes AGO2/miRNA complexes. PMID: 27486976
Data suggest neuropilin-1 (NRP-1) as a therapeutic target to reduce tumor fibrosis and pancreatic ductal adenocarcinoma (PDAC) progression. PMID: 27542226
Results suggest that targeting the transmembrane domains (TMD) of neuropilin-1 (NRP1) in breast cancer is a potent new strategy to fight against breast cancer and related metastasis. PMID: 27351129
By blocking neuropilin-1 receptor (NRP-1) in tumor-bearing mice, luciferase activity in tumors delivered by HK polyplexes was reduced by 96%, whereas activity in normal tissues was minimally reduced. PMID: 27257039
High NRP1 expression is associated with metastasis in triple-negative breast cancer. PMID: 26967387
Overexpression of Neuropilin 1 incresed in breast cancer stem cells formation and breast cancer stem cells-induced tube formation and knockdown of Neuropilin 1 reduced CSCs-related traits. PMID: 29179185
Nrp1 mediates the migratory behavior of mesangial cells. PMID: 28835419
this study identified the NRP1 cytoplasmic domain as essential for acute vascular hyperpermeability induced by different NRP1 ligands: a ligand- blocking antibody against NRP1 and a CendR peptide. PMID: 27117252
Data indicate that miR-206 inhibits neuropilin-1 (NRP1) and SMAD2 gene expression by directly binding to their 3'-UTRs. PMID: 27014911
Results show that Nrp1 plays a critical role in balancing responsiveness to VEGF-A versus TGFbeta to regulate glioblastoma growth, progression, and recurrence after anti-vascular therapy. PMID: 28938007
Data provide evidence that NRP1 functions to enhance the metastatic potential of prostate tumors. PMID: 28092670
NRP1 is an important niche component PMID: 27676403
our data suggest a novel molecular mechanism by which tMUC1 may modulate NRP1-dependent VEGFR signaling in PDA cells. PMID: 26804176
Our findings identify aberrant active integrin b1-driven Src-Akt hyperactivation as a primary resistance mechanism to cetuximab in PDAC cells and offer an effective therapeutic strategy to overcome this resistance using an EGFR and NRP1 dual targeting antibody PMID: 27797376
VEGF-A acts via interaction with NRP-1 to trigger intracellular events leading to ECS cell survival and formation of aggressive, invasive and highly vascularized tumors. PMID: 26804163
These data identify a new molecular mechanism of brain microvascular endothelial inflammatory response through NRP1-IFNgamma crosstalk. PMID: 27591257
This study indicates that capsaicin application results in significant loss of epidermal NRP-1 receptor expression, whereas diabetic subjects presenting small fiber neuropathy show full epidermal NRP-1 expression in contrast to the basal expression pattern seen in healthy controls PMID: 27598321
Study show that a high percentage of intratumoral NRP1(+) Tregs correlates with poor prognosis in melanoma and head and neck squamous cell carcinoma. PMID: 28552348
Study revels the structural description of the neuropilin-1 ectopic region by reporting the crystal structure of its MAM domain. The domain adopts a jellyroll fold that is stabilized by a Ca2+ ion while forming a molecular surface that is distinct from its structural homologs. PMID: 27720589
Data suggest that neuropilin-1 (NRP1) functions as a receptor for neutrophil elastase (NE), mediates uptake/absorption of NE, and facilitates cross-presentation of PR1 (a peptide fragment of NE) in breast cancer cells. PMID: 28468826
This report is the first study characterizing the specific functions of NRP1-Delta7, a glycosaminoglycan-defective neuropilin-1 splice variant that displays anti-tumorigenic properties in vitro and in vivo. PMID: 27798666
this study shows that NRP1 can act as a co-receptor for PDGF-D-PDGFRbeta signaling and is possibly implicated in intercellular communication in the vascular wall PMID: 28254885
This review provides a general overview of current knowledge of the signalling pathways that are modulated by NRP1, with particular focus on neuronal and vascular roles in the brain and retina. PMID: 26923176
Curcumin-loaded nanoliposomes linked to homing peptides for integrin targeting and neuropilin-1-mediated internalization reduced viability of breast cancer cells. PMID: 27937055
Upregulated expression of NRP1 is associated with glioma. PMID: 27481513
Studies suggest that the activation of NRP-1 by PlGF directly contributes to tumour aggressiveness and to melanoma escape from anti-VEGF-A therapies. PMID: 26846845
REVIEW: Nrp1 functions in the vasculature is critical for the development of targeted therapeutics for cancer and vascular diseases such as atherosclerosis and retinopathies. PMID: 26849476
High Expression of Neuropilin-1 Associates with Hepatocellular Carcinoma. PMID: 26563279
Increased NRP-1 expression is associated with metastatic endometrial and lung cancers. PMID: 26701889
miR-152 suppression in NSCLC cells might promote neuropilin-1 mediated cancer metastasis. PMID: 26823738
NRP-1 was found to be overexpressed in gastric cancer (GC) tissues, and its expression correlates with the clinical staging, tumor differentiation and pathological types of gastric cancer. PMID: 26795388
NRP1 was targeted by miR130a and miR130b at the binding site of chromosome 10: 334668643466870, which was involved in the axon guidance signaling pathway. PMID: 26573160
Semaphorin-3a, neuropilin-1 and plexin-A1 are axonal guidance molecules that have been recently implicated in regulating bone metabolism. PMID: 26602825
neuropilin-1 is regulated in the oral epithelium and is selectively up-regulated during epithelial dysplasia PMID: 26877262
NRP1 over-expression in miR-365 expressing cells could rescue invasion and growth defects of miR-365. In addition, miR-365 expression inversely correlated with NRP1 protein levels in malignant melanoma PMID: 26406949
Data show that binding of pleiotrophin (PTN) to neuropilin-1 (NRP-1) stimulated the internalization and recycling of NRP-1 at the cell surface. PMID: 26408254
Study found significant down-regulation of placental NRP-1 expression in fetal growth restriction pregnancies complicated with absent end-diastolic flow in the umbilical artery. PMID: 26409917

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Subcellular Location

[Isoform 2]: Secreted.; Mitochondrion membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Cytoplasm.

Protein Families

Neuropilin family

Tissue Specificity

[Isoform 1]: The expression of isoforms 1 and 2 does not seem to overlap. Expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and

Database Links

HGNC: 8004

OMIM: 602069

KEGG: hsa:8829

STRING: 9606.ENSP00000265371

UniGene: Hs.131704

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Code	CSB-RA016091MA1HU
Size	US$210
	Order now
Image	Overlay Peak curve showing PC3 cells stained with CSB-RA016091MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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NRP1 Recombinant Monoclonal Antibody

Product Details

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Customer Reviews and Q&A

Target Background