Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human TFRC at 2 μg/mL can bind Anti-TFRC recombinant antibody (CSB-RA023441MA1HU) , the EC50 is 3.305-8.220 ng/mL.

Target Names

TFRC

Uniprot No.

P02786

Alternative Names

Transferrin receptor protein 1; TR; TfR; TfR1; Trfr; T9; p90

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

89-760aa

Target Protein Sequence

CKGVEPKTECERLAGTESPVREEPGEDFPAARRLYWDDLKRKLSEKLDSTDFTGTIKLLNENSYVPREAGSQKDENLALYVENQFREFKLSKVWRDQHFVKIQVKDSAQNSVIIVDKNGRLVYLVENPGGYVAYSKAATVTGKLVHANFGTKKDFEDLYTPVNGSIVIVRAGKITFAEKVANAESLNAIGVLIYMDQTKFPIVNAELSFFGHAHLGTGDPYTPGFPSFNHTQFPPSRSSGLPNIPVQTISRAAAEKLFGNMEGDCPSDWKTDSTCRMVTSESKNVKLTVSNVLKEIKILNIFGVIKGFVEPDHYVVVGAQRDAWGPGAAKSGVGTALLLKLAQMFSDMVLKDGFQPSRSIIFASWSAGDFGSVGATEWLEGYLSSLHLKAFTYINLDKAVLGTSNFKVSASPLLYTLIEKTMQNVKHPVTGQFLYQDSNWASKVEKLTLDNAAFPFLAYSGIPAVSFCFCEDTDYPYLGTTMDTYKELIERIPELNKVARAAAEVAGQFVIKLTHDVELNLDYERYNSQLLSFVRDLNQYRADIKEMGLSLQWLYSARGDFFRATSRLTTDFGNAEKTDRFVMKKLNDRVMRVEYHFLSPYVSPKESPFRHVFWGSGSHTLPALLENLKLRKQNNGAFNETLFRNQLALATWTIQGAANALSGDVWDIDNEF

Mol. Weight

76.5 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant human transferrin receptor protein 1 (TFRC) was expressed in the mammalian cells. The human TFRC protein gene encoding fragment (89-760aa) fused with the 10xHis-tag gene at the N-terminus is cloned into a plasmid vector and then transfected into the mammalian cells. The transfected mammalian cells start expressing the recombinant TFRC protein, which is collected from the cell lysate. The purified recombinant TFRC protein undergoes rigorous quality control testing to ensure its identity, purity, activity, and endotoxin levels. Its purity is greater than 95% determined by SDS-PAGE and endotoxin content is less than 1.0 EU/ug measured by the LAL method. In a functional ELISA, immobilized human TFRC at 2 μg/mL can bind the anti-TFRC recombinant antibody (CSB-RA023441MA1HU), with the EC₅₀ of 3.305-8.220 ng/mL.

TFRC, as an essential mediator of normal iron metabolism in the human body, primarily assists in the transport of transferrin inside and outside the cells and across the blood-brain barrier, maintaining cellular iron balance. TFRC is lowly expressed in all normal nuclear cells, but exhibits higher expression levels in immature proliferating cells and significantly increased expression in tumor cells. The upregulation of TFRC expression in malignant cells and its central role in cancer pathology make TFRC an attractive target for antibody-mediated cancer therapy. TFRC has also been an important target receptor for drug transport across the blood-brain barrier. By targeting TFRC on the surface of the blood-brain barrier, specific drugs can be delivered to the brain parenchyma, potentially improving the treatment of diseases such as Alzheimer's, Parkinson's, and acute stroke. Developing active mammalian TFRC proteins is of great significance in further studying TFRC's function and developing tumor drugs.

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Target Background

Function

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake. Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway. When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion. When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2.; (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.

Gene References into Functions

TFR1 was increased in myelodysplastic syndrome patients and expression was upregulated in CD34+ cells from patients with refractory anaemia with ringed sideroblasts (RARS). PMID: 28714470
palmitoylation is reported as a hitherto unreported level of post-translational TfR1 regulation. PMID: 29395073
Our data provide evidence that blocking TfR could significantly inhibit lung adenocarcinoma (LAC) proliferation by targeting the oncogene KRAS; therefore, TfR may be a therapeutic target for LAC. In addition, our results suggest a new method for blocking the signal from the oncogene KRAS by targeting TfR in LAC. PMID: 29286585
TfR could be used as a marker of erythropoiesis in high-flux hemodialysis patients PMID: 28446125
The results suggest a role of microRNAs in the TfR1 regulation in the iron-regulatory protein-iron-responsive element system. PMID: 29295890
Cell viability and surface expression of transferrin receptor (CD71) and glycophorin A (GPA) were analyzed before and after re-culture by flow cytometry. These studies show differential sensitivities of these surface proteins on K562 cells to proteases, and suggest molecular mechanisms of transmembrane protein transport and cycling. PMID: 28980921
These data show that TfR1-PvRBP2b invasion pathway is critical for the recognition of reticulocytes during P. vivax invasion. PMID: 29302006
combination of desferal with oxaliplatin can overcome cervical cancer oxaliplatin resistance through the regulation of hCtr1 and TfR1 PMID: 27384479
Data suggest that hematologic parameters in children consuming lacto-ovo vegetarian diets are comparable with those of control children, but ferritin levels are lower; inclusion of novel serum biomarkers, soluble transferrin receptor and hepcidin, in nutritional assessment can better detect subclinical iron deficiency in children following vegetarian diet. This study was conducted in Poland with children ages 4.5-9 years. PMID: 28342014
results suggest that lower Hepcidin-25, as well as higher sTfR and sTfR/Hepcidin-25 ratio were significant predictors of favorable hemoglobin response within a month after IV administration of ferric carboxymaltose in patients with CKD PMID: 27282576
TfR1 was highly expressed in glioblastomas and associated with shorter survival in the whole cohort, but not in the individual malignancy grades PMID: 28837569
Expression of Hepcidin and Ferroportin in the Placenta, and Ferritin and Transferrin Receptor 1 Levels in Maternal and Umbilical Cord Blood in Pregnant Women with and without Gestational Diabetes PMID: 27483296
Human melanoma cells are able to up-regulate TFRC expression using hyaluronan/CD44 signaling. PMID: 28551638
the group with homozygous or compound heterozygous state for beta-thalassemia had the highest sTfR1 levels and that the presence of increased sTfR1 levels (>5 times normal) was associated with a complex and severe history of disease requiring splenectomy, occasional red blood cells transfusions, and early start and continuous iron chelation therapy PMID: 28707012
high expression of transferrin receptor-1 resulting in iron uptake contributes to increase in the labile iron pool which plays roles in cholangiocarcinoma progression with aggressive clinical outcomes. PMID: 28671021
EGFR regulates iron homeostasis to promote non-small cell lung cancer growth through redistribution of transferrin receptor 1. PMID: 27523281
TFR1 is overexpressed in adult T-cell leukemia/lymphoma caells . PMID: 28189691
NSF deficiency in HeLa cells barely affected cell viability, anterograde trafficking of vesicular stomatitis virus glycoprotein G and transferrin endocytosis. PMID: 27995606
inhibition of c-Abl minimizes receptor recycling pathways and results in chaperone-dependent trafficking of the TfR1 to the lysosome for degradation. PMID: 27226592
alpha6(high)/CD71(low) cells give rise to a thicker pluristratified epithelium with lower seeding density and display a low Ki67 positive cells number, showing that they have reached the balance between proliferation and differentiation. PMID: 28134816
Data show that the concentration of soluble transferrin receptor with high diagnostic value in the detection of iron deficiency-based anemia in patients undergoing dialysis for chronic renal failure at the end-stage. PMID: 27629729
inverse correlation between the total percentage of CD71-positive NRBCs and gestational age suggests that this analysis may help in pregnancy dating PMID: 25906120
Data indicate an over-expression of transferrin receptor 1 (TFRC) in hepatocellular carcinoma (HCC) and a markedly decreased level of microRNA-152 (miR-152) when compared to non-tumor liver tissue. PMID: 26657500
Together, our findings reveal that TfR1 can contribute to the mitochondrial respiration and ROS production, which have essential roles in growth and survival of pancreatic cancer. PMID: 26869514
siRNA targeted delivery using transferrin-coupled lipoplexes specifically sensitizes CD71 high expressing malignant cells to antibody-mediated complement attack PMID: 25395366
Case Report: cellular iron deficit due to the low level of transferrin receptor, particularly in erythroid tissue. PMID: 26339443
The requirement for a threshold level of TFR1 expression can explain why among primary human hematopoietic cells, only erythroblasts efficiently take up H-ferritin. PMID: 26441243
Data indicate only the glycoproteins CD34+/CD71low population showed statistically significant differences between Diamond-Blackfan Anaemia (DBA) patients and controls. PMID: 26394034
These results demonstrate that in cells expressing the hTfR, Tacaribe virus internalization depends on the presence of cholesterol, dynamin and acidic intracellular vesicles. PMID: 26559962
The importance of TfR1 in adaptive immunity.A missense mutation in TFRC, encoding transferrin receptor 1, causes combined immunodeficiency. PMID: 26642240
The blood levels of TfRC was higher in age-related macular degeneration patients than controls.No association was found between age-related macular degeneration occurrence and the p.Gly142Ser polymorphism of the TRFC gene. PMID: 25915522
Data show that interleukin 6 (IL-6) secretion and the percentage of CD71 antigen-positive T lymphocytes both uniquely predicted survival independent of papillomavirus infection status. PMID: 26079381
This study provides insights into the effector functions of human IgG3 in the context of an antibody targeting TfR1. PMID: 26232328
this report provided a convenient protocol that could be fulfilled in order to prepare hTfR1 inclusion body, which failed to be purified by an Ni(2+) affinity column. PMID: 25207990
elucidation of a signalling pathway whereby C18:0 stearoylates TFR1, thereby inhibiting its activation of JNK signaling; this leads to reduced ubiquitination of mitofusin via HUWE1, thereby promoting mitochondrial fusion and function PMID: 26214738
The surface levels of three receptors [TfR, epidermal growth factor receptor (EGFR) and beta1 integrin] were tested and found to be reduced dependent on Escherichia coli EspG translocation. PMID: 24898821
High TFRC expression is associated with response to low intravenous immunoglobulin therapy in patients with Kawasaki disease. PMID: 25449331
The rate of TFRC expression in oral sqamous cell neoplasms was significantly higher than that in dysplasia, suggesting that it may be involved in disease progression. PMID: 24890018
CD71 is useful for the evaluation of differentiation stage for AML cells. PMID: 23962073
these findings suggest that TFR1 has an important role in sphingosine kinase 1-mediated oncogenesis. PMID: 24276247
a critical review the available literature to assess the diagnostic efficacy of sTfR in complicated anemia--{REVIEW} PMID: 24525213
Expression of the transferrin receptor CD71 is associated with clinical features of esophageal squamous cell carcinoma. PMID: 24435655
results suggest that Transferrin or a TF-TFR2 complex may have a role in the etiology of PD, possibly through iron misregulation or mitochondrial dysfunction within dopaminergic neurons. PMID: 24121126
high levels of TfRs such as those found on activated lymphocytes were found to be associated with decreased KLRG1 inhibitory function, indicating that TfRs may sequester KLRG1 from interacting with cadherins. PMID: 24752778
MARCH 8 mediates the ubiquitination and lysosomal degradation of the transferrin receptor. PMID: 23606747
A statistically significant difference in urinary sTfR concentration was observed between patients with active IgA nephropathy or Henoch-Schonlein purpura nephritia and patients who had achieved partial or complete remission PMID: 23384534
The kinesin KIF16B mediates apical transcytosis of transferrin receptor in AP-1B-deficient epithelia. PMID: 23749212
Knockdown of Rab12 increased transferrin receptor level and reduced M98K-induced cell death. PMID: 23357852
TfR1 plays a role in hepatitis C virus (HCV) infection at the level of glycoprotein-mediated entry, acts after CD81, and possibly is involved in HCV particle internalization. PMID: 23754414
Among the PC family members, only furin activates hepcidin in hepatocytes, and uniquely the full-length membrane-bound PC7 can directly shed hTfR1 by cleavage at Arg100 PMID: 23390091

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Involvement in disease

Immunodeficiency 46 (IMD46)

Subcellular Location

Cell membrane; Single-pass type II membrane protein. Melanosome.; [Transferrin receptor protein 1, serum form]: Secreted.

Protein Families

Peptidase M28 family, M28B subfamily

Database Links

HGNC: 11763

OMIM: 190010

KEGG: hsa:7037

STRING: 9606.ENSP00000353224

UniGene: Hs.529618

Code	CSB-MP3648HU
MSDS	MSDS Datasheet
Size	$104
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Human TFRC at 2μg/mL can bind Anti-TFRC recombinant antibody (CSB-RA023441MA1HU)， the EC₅₀ is 3.305-8.220 ng/mL. Biological Activity Assay
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Recombinant Human Transferrin receptor protein 1 (TFRC), partial (Active)

Product Details

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Target Background

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