Recombinant Human Trefoil factor 3 protein(TFF3) (Active)

In Stock
Code CSB-AP000361HU
Size US$3274Purchase it in Cusabio online store
(only available for customers from the US)
Image
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity >97% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 μg/ml, corresponding to a specific activity of >100 IU/mg.
Target Names TFF3
Uniprot No. Q07654
Research Area Signal Transduction
Alternative Names hITF; hP1.B; Intestinal trefoil factor; ITF; mITF; OTTMUSP00000021729; P1B; Polypeptide P1.B; TFF3; TFF3_HUMAN; TFI; TREFOIL; Trefoil factor (intestinal); Trefoil factor 3
Species Homo sapiens (Human)
Source E.Coli
Expression Region 22-80aa
Complete Sequence EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF
Mol. Weight 6.6 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 150mM NaCl
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
Gene References into Functions
  1. TFF3 located in the renal tubular epithelial cells may play a role in the progression of tubulointerstitial fibrosis in IgA nephropathy patients. PMID: 29987860
  2. Authors hypothesised that TFF3 may involve regulatory networks of ncRNAs to exert its effects as a scattering factor. PMID: 28277538
  3. The highly specific expression of TFF3 in mucinous ovarian cancer may help to further clarify potential relationships of tumors with mucinous histology PMID: 28470574
  4. This is, to our knowledge, the first report of decreased expression of TFF3, SPDEF, KLF4, and goblet cell population in the colon of patients with HSCR. Altered goblet cell function may result in intestinal barrier dysfunction contributing to the development of HAEC. PMID: 29383490
  5. High TFF3 expression is associated with oncogenicity and chemoresistance in hepatocellular carcinoma. PMID: 28445151
  6. Results indicate diagnostic biomarker potential of Trefoil factor 3 (TFF3) promoter hypomethylation for prostate cancer (PC) as well as prognostic biomarker potential of TFF3 RNA expression. PMID: 28930171
  7. ITF role in promoting intestinal epithelial cells migration through crosstalk between the ERK and JAK/STAT3 pathways PMID: 27616044
  8. TFF3 levels were significantly elevated in patients with stage I (p= 0.001) and T1 gastric cancer (p= 0.013). PMID: 28453457
  9. The present study demonstrated a negative association between the expression of miR75p and TFF3 in IBD lesional tissues and normal tissues. In conclusion, TFF3 was identified as a novel target of miR75p PMID: 28627600
  10. trefoil factors family 3 may have a role in colorectal cancer PMID: 28405143
  11. This study provides the first evidence that TFF3 is locally elevated in the peritoneal cavity in endometriosis and might play a role in disease pathogenesis and its associated inflammatory processes. Furthermore, the results show that TFF3 is regulated through the menstrual cycle. PMID: 27330011
  12. involvement of TFF3 in the pathogenesis of rhonchopathy and OSA PMID: 29028798
  13. Giardia co-infection promotes the secretion of antimicrobial peptides beta-defensin 2 and trefoil factor 3 and attenuates attaching and effacing bacteria-induced intestinal disease.( PMID: 28622393
  14. expression reduces growth, viability, and tumorigenicity of retinoblastoma cell oines PMID: 27626280
  15. The expression of TFF3 and TWIST1 might be associated with the survival of patients with colorectal cancer (CRC) after curative resection and might be pivotal predictors of disease progression. TFF3 may be correlated to the invasiveness of CRC. PMID: 28104986
  16. Serum TFF-3 is not a convenient and reliable surrogate marker of mucosal healing during therapy with TNF-alpha antagonists in Crohn's disease. PMID: 28104989
  17. TFF3 levels differed among idiopathic pulmonary fibrosis, pulmonary sarcoidosis and chronic pulmonary obstructive disease. PMID: 27758987
  18. TFF3 protein knockout can facilitate apoptosis of human pituitary adenoma HP75 cells via mitochondrial pathway PMID: 26823779
  19. Data show that trefoil factor-3 (TFF3) secreted from mammary carcinoma cells promotes de novo angiogenesis via interleukin-8 (IL-8)/IL-8 receptor CXCR2. PMID: 26559818
  20. Dimers CXCR4 and CXCR7 are involved in TFF3-dependent activation of cell migration, but not cell proliferation. The ERK1/2 pathway is activated in the process, but not influenced by CXCR4 or CXCR7. PMID: 26780310
  21. TFF3 expression is associated with response to endocrine therapy, and outperforms oestrogen receptor, progesterone receptor and TFF1 as an independent biomarker, possibly because it mediates the malign effects of oestrogen on invasion and metastasis PMID: 25900183
  22. the results provide a mechanistic explanation for ITF-mediated protection of gastric epithelial mucosa cells, suggesting that activation of the ERK1/2 signaling pathway may provide a new therapeutic strategy for repairing gastric injury. PMID: 25776570
  23. primary outcome measures were to evaluate the safety, acceptability, and accuracy of the Cytosponge-TFF3 test compared with endoscopy and biopsy. In all, 1,042 (93.9%) patients successfully swallowed the Cytosponge, and there were no serious side effects PMID: 25634542
  24. TFF3 is not only a useful biomarker for a long-term surgical result in CRC patient, but also may be a risk factor of early recurrence. PMID: 25405728
  25. Associations discovered between trefoil factor 3 gene variants and idiopathic recurrent spontaneous abortion. PMID: 25444508
  26. TFF3 expression predicts metastasis and poor survival outcome of patients with mammary carcinoma. PMID: 25266665
  27. TFF3 expression may be absent in the early stages of Barrett's esophagus and its expression may represent more established columnar epithelium. PMID: 25424203
  28. This study suggests that TFF3 is an independent indicator for survival in GC, while HER2 is not associated with the outcome. Patients with HER2-negative/TFF3-negative GC have the best outcome. PMID: 25514872
  29. High TFF3 expression is associated with reduces sensitivity to ionising radiation in prostate carcinoma. PMID: 25748388
  30. Study supports the notion that ITF can promote cell proliferation, migration and preserve epithelium integrity. PMID: 24990304
  31. LRP2 sequencing reveals multiple rare variants associated with urinary trefoil factor-3 PMID: 24876117
  32. Serum concentrations of TFF3 is significantly higher in chronic kidney disease. PMID: 24282531
  33. TFF3 expression at both mRNA and protein levels was significantly downregulated by ErbB-2 silencing in breast cancer cell lines PMID: 24164280
  34. A significantly higher trefoil factor 3 expression in luminal epithelial cells was detected in women with unexplained infertility PMID: 24228773
  35. TFF3 point mutations seem to be a rare event in colorectal carcinogenesis; TFF3 expression may play a role in promoting lymph node metastases of CRCs. PMID: 24125969
  36. TFF3 is an independent indicator for overall survival in gastric cancer. PMID: 23822993
  37. In serum TFFs and pepsinogen tests, only serum TFF3s were not significantly affected by H. pylori eradication, suggesting that serum TFF3 could be a stable biomarker of gastric cancer risk even after H.pylori eradication PMID: 22907485
  38. TFF3 hypermethylation is associated with triple-negative breast cancers. PMID: 24045095
  39. interaction between DMBT1(gp340) and trefoil TFFs proteins was investigated using an ELISA assay. DMBT1(gp340) bound to solid-phase bound recombinant dimeric TFF3 in a calcium dependent manner PMID: 23691218
  40. ER+ /PR+ /TFF3+ /IMP3- immunoprofile distinguishes endometrioid from serous and clear cell carcinomas of the endometrium. PMID: 23570281
  41. Using a Caco-2 cell model the study showed that TLR2 and TFF3 inhibit the IL-1beta induced increase in permeability and release of proinflammatory cytokines, and that this effect is mediated by activation of PI3K/Akt signaling. PMID: 24051092
  42. Distinct ERG and TFF3 protein was expressed in 45% (43/96) and 36% (35/96) of prostate cancers, respectively. PMID: 23856515
  43. Our study suggests that Twist, as an important potential downstream effector, plays a key role in TFF3-modulated metastasis in gastric cancer and can be a promising therapeutic target against intestinal-type gastric cancer. PMID: 23845905
  44. TFF3 activated the epithelial cells in culture to produce beta defensins 2 (hBD2) and beta defensins 4 PMID: 23198942
  45. TFF3 seems to be a novel pathway in the pathogenesis of type I endometrial carcinomas. PMID: 23578537
  46. Upregulation of TFF3 after chemoradiotherapy for rectal cancer is associated with a higher risk of relapse. The physiological role of TFF3 in restoring the mucosa during CRT could be interfering with treatment efficacy. PMID: 22516806
  47. Measurement of TFF3 mRNA in aspirates from thyroid nodules using mesh filtration. PMID: 22572548
  48. TFF3 may contribute to ocular surface wound healing after Amniotic membrane transplantation, but its production by AM is not further inducible by proinflammatory stimuli. PMID: 22476621
  49. Report TFF3 expression in normal/diseased pancreas. PMID: 22286382
  50. Differential expression of MUC5AC involves crosstalk between interleukin-1beta and Akt, whereby the trefoil factor family peptide TFF3 activates Akt by phosphorylation of EGFR. PMID: 22389405
  51. These data demonstrated the TFF3 mediated regulation of VEGF expression induced by hypoxia. PMID: 21769478
  52. results show increased TFF3 levels in serum and lung tissues, suggesting that TFF3 may serve as a promising, easily detected biomarker of lung cancer. PMID: 22246423
  53. TFF3 is expressed in both the normal and diseased breast and although associated with features of good prognosis, its profile of expression in invasive cancer is consistent with a role in breast tumor progression and tumor cell dissemination. PMID: 22341453
  54. Trefoil factor 3 predicts incident chronic kidney disease PMID: 21829008
  55. TFF3, TMPRSS2 and ERG were up-regulated in the prostate carcinoma glands compared to the corresponding normal glands. PMID: 21743959
  56. TFF3 gene expression was increased in the terminal ileum. Immunohistochemistry showed that some goblet cells did not include TFF3 in the jejunum and that TFF3-positive goblet cells increased toward the terminal ileum. PMID: 21125297
  57. Gene-expression data suggest a difference in expression between STT3A, Clorf24, and TFF3 in FAs versus carcinomas that may be detected from an FNA sample. Findings must be validated from preoperative FNAs in larger numbers PMID: 21520112
  58. ERG has an inhibitory effect on TFF3 expression in hormone-naive prostate cancer but not in castration-resistant state; TFF3 enhances ERG-mediated cell invasion in castration-resistant prostate cancer cells resulting in enhanced tumor cell aggressiveness PMID: 21170267
  59. TFF3 is oncogenic, predicts outcome of estrogen receptor (ER)-positive breast cancer patients treated with tamoxifen, and mediates anti-estrogen resistance in mammary carcinoma. PMID: 21170268
  60. The expression of hITF mRNA is increased in Crohn's disease PMID: 20499178
  61. Overexpression and promoter hypomethylation of TFF3 is associated with prostate cancer. PMID: 20112343
  62. Data show that expression of intestinal trefoil factor is significantly related to gastrointestinal functional failure in critical illness children. PMID: 17217669
  63. This is the first study to correlate ITF expression with clinicopathological features or outcome in any cancer type. PMID: 12006524
  64. expression of ITF may play an important role in bile duct damage because in small bile ducts, production is absent, making such cells vulnerable and providing a thesis for the loss of small, but not large, bile ducts in primary biliary cirrhosis. PMID: 12395334
  65. secreted trefoil factor 3 is not deficient in premature infants and can be detected by immunohistochemistry in human intestine as early as 12 weeks gestation PMID: 12663147
  66. The solution structure of the TFF3 dimer has been determined and compared with the structure of the TFF3 monomer and with other trefoil dimer structures (TFF1 and TFF2) and is found to be markedly different from other trefoil proteins. PMID: 14690424
  67. TFF3 has important physiological functions in the stomach, e.g. as a luminal surveillance peptide maintaining particularly the integrity of the antral and pyloric mucosa. PMID: 14968359
  68. decreased expression of TFF3 mRNA is a marker of follicular thyroid carcinomas, especially those with a high risk of invasion or metastasis PMID: 15083192
  69. Decreases in the CRABP1 (cellular retinoic acid binding protein 1) and TFF3 (trefoil factor 3) expression levels identified these as candidate molecular biomarkers for papillary thyroid carcinoma. PMID: 15515157
  70. TFF3 overexpression may be a critical process in mouse and human hepatocellular carcinogenesis PMID: 15645121
  71. PI3-K, but not the Stat6 promotes the expression of TFF3 and MUC2 and that the PI3-K pathway may play a pivotal role in intestinal goblet cell differentiation. PMID: 15733066
  72. TFF-3 is commonly expressed in hepatocellular carcinoma and its expression correlates with tumor grade PMID: 16110118
  73. Expression of TFF3 had a significant correlation with patient age, the number and the area of microvessels. PMID: 16166422
  74. The expression of TFF3 in chronic graft-versus-host disease of the liver is increased in response to bile duct damage and repair. PMID: 16278592
  75. Van Gogh-like protein 1 (VANGL) is serine/threonine phosphorylated in response to ITF stimulation PMID: 16410243
  76. These results indicate that TFF3 is able to induce ciliogenesis and to promote airway epithelial ciliated cell differentiation, in part through an epidermal growth factor receptor-dependent pathway. PMID: 17008636
  77. Plays a role in the regulation of intestinal barrier function by altering the claudin composition within tight junctions, thus decreasing paracellular permeability of the intestinal mucosa. PMID: 17018241
  78. This is the first report of TFF3 as a typical secretory peptide of esophageal submucosal glands and gastric cardia. PMID: 17216196
  79. TFF3, in contrast to epidermal growth factor, enhanced a collective cell migration ensuring a precise coverage of the re-populated area avoiding gaps PMID: 17762162
  80. Findings reveal a novel TFF3-mediated pathway for stimulation of beta-cell replication that could ultimately be exploited for expansion or preservation of islet beta-cell mass. PMID: 18258687
  81. reveal a pivotal role for Tff3 in corneal wound healing mechanism and have broad implications for developing novel therapeutic strategies for treating nonhealing wounds PMID: 18326859
  82. is secreted from the submandibular gland and is present in whole saliva; increased the migration of both normal oral keratinocytes and the cancer cell line D12; is likely to contribute to oral wound healing PMID: 18353006
  83. Development of TFF3-based diagnostic methods is now ongoing and it may not be long before thyroid follicular carcinoma can be diagnosed preoperatively using an aspirated sample from the tumor. PMID: 18506086
  84. Patients harbouring G3-endometrioid endometrial carcinomas had significantly higher TFF3 serum concentration by ELISA when compared with healthy patients or patients harbouring endometrial hyperplasia PMID: 18682706
  85. TFF3 contributes to the malignant behavior of colon cancer cells. PMID: 18979216
  86. hTFF3 administered to mice decreased tissue TNF-alpha, TLR4, NF-kappaB production, and TLR4, NF-kappaB mRNA expression PMID: 19238529
  87. Follicular neoplastic lesions of the thyroid gland have decreased expression of TFF3 compared to normal thyroid tissue, suggesting that TFF3 may have a role in normal thyroid tissue. PMID: 19528408

Show More

Hide All

Subcellular Location Secreted, extracellular space, extracellular matrix, Cytoplasm
Tissue Specificity Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus. Also expressed in the hypothalamus where it is detected in paraventricular, periventricular
Database Links

HGNC: 11757

OMIM: 600633

KEGG: hsa:7033

STRING: 9606.ENSP00000430690

UniGene: Hs.82961

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1