Purity
Greater than 90% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized TNFRSF14 at 5 μg/ml can bind human TNFSF14(CSB-MP023991HUj2), the EC50 is 49.85-79.31 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF14 at 5 μg/ml can bind Biotinylated human TNFSF14 (CSB-MP023991HUj7-B), the EC50 is 1.773-3.707 ng/ml.
Alternative Names
HVEML; ATAR; CD270; CD40 like protein precursor; Herpes virus entry mediator A; Herpesvirus entry mediator A; Herpesvirus entry mediator; Herpesvirus entry mediator ligand; HveA; HVEM; HVEM L; LIGHT; LIGHTR; TNFRSF14; TNFSF 14; TNR14_HUMAN; TR2; Tumor necrosis factor receptor like gene2; Tumor necrosis factor receptor superfamily member 14; Tumor necrosis factor receptor superfamily member 14 precursor; Tumor necrosis factor receptor-like 2
Species
Homo sapiens (Human)
Expression Region
39-202aa
Target Protein Sequence
LPSCKEDEYPVGSECCPKCSPGYRVKEACGELTGTVCEPCPPGTYIAHLNGLSKCLQCQMCDPAMGLRASRNCSRTENAVCGCSPGHFCIVQDGDHCAACRAYATSSPGQRVQKGGTESQDTLCQNCPPGTFSPNGTLEECQHQTKCSWLVTKAGAGTSSSHWV
Tag Info
C-terminal hFc1-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
A DNA fragment corresponding to the peptide of human TNFRSF14 was expressed with a C-terminal hFc-tag in mammalian cells. TNFRSF14 CSB-MP842173HU is a truncated molecule having amino acids of Leu39-Val202. Its purity is greater than 90% measured by SDS-PAGE. A molecular mass band of about 50 kDa was presented on the gel under reducing conditions. Its endotoxin level is less than 1.0 EU/ug determined by the LAL method. Through the functional ELISA, this TNFRSF14 protein has been validated as an active protein. And it is available now.
TNFRSF14, also known as CD270, LIGHTR, or HVEM, is mainly expressed in hematopoietic and lymphoid tissues. The ligation of TNFRSF14 and its ligands is involved in phagocytosis enhancement, anti-bacterial inflammation, and T cell co-stimulation or immunosuppression. TNFRSF14 also delivers a negative signal to T cells through B and T Lymphocyte Attenuator (BTLA) molecule and has been linked to a poor prognosis in various tumors.
