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| Code | CSB-MP842173HU |
| Size | $428 |
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| Description |
A DNA fragment corresponding to the peptide of human TNFRSF14 was expressed with a C-terminal hFc-tag in mammalian cells. TNFRSF14 CSB-MP842173HU is a truncated molecule having amino acids of Leu39-Val202. Its purity is greater than 90% measured by SDS-PAGE. A molecular mass band of about 50 kDa was presented on the gel under reducing conditions. Its endotoxin level is less than 1.0 EU/ug determined by the LAL method. Through the functional ELISA, this TNFRSF14 protein has been validated as an active protein. And it is available now. TNFRSF14, also known as CD270, LIGHTR, or HVEM, is mainly expressed in hematopoietic and lymphoid tissues. The ligation of TNFRSF14 and its ligands is involved in phagocytosis enhancement, anti-bacterial inflammation, and T cell co-stimulation or immunosuppression. TNFRSF14 also delivers a negative signal to T cells through B and T Lymphocyte Attenuator (BTLA) molecule and has been linked to a poor prognosis in various tumors. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Endotoxin | Less than 1.0 EU/ug as determined by LAL method. |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized TNFRSF14 at 5 μg/ml can bind human TNFSF14(CSB-MP023991HUj2), the EC50 is 49.85-79.31 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF14 at 5 μg/ml can bind Biotinylated human TNFSF14 (CSB-MP023991HUj7-B), the EC50 is 1.773-3.707 ng/ml. |
| Target Names | TNFRSF14 |
| Uniprot No. | Q92956 |
| Research Area | Cancer |
| Alternative Names | HVEML; ATAR; CD270; CD40 like protein precursor; Herpes virus entry mediator A; Herpesvirus entry mediator A; Herpesvirus entry mediator; Herpesvirus entry mediator ligand; HveA; HVEM; HVEM L; LIGHT; LIGHTR; TNFRSF14; TNFSF 14; TNR14_HUMAN; TR2; Tumor necrosis factor receptor like gene2; Tumor necrosis factor receptor superfamily member 14; Tumor necrosis factor receptor superfamily member 14 precursor; Tumor necrosis factor receptor-like 2 |
| Species | Homo sapiens (Human) |
| Source | Mammalian cell |
| Expression Region | 39-202aa |
| Target Protein Sequence | LPSCKEDEYPVGSECCPKCSPGYRVKEACGELTGTVCEPCPPGTYIAHLNGLSKCLQCQMCDPAMGLRASRNCSRTENAVCGCSPGHFCIVQDGDHCAACRAYATSSPGQRVQKGGTESQDTLCQNCPPGTFSPNGTLEECQHQTKCSWLVTKAGAGTSSSHWV |
| Mol. Weight | 48.5 kDa |
| Protein Length | Partial |
| Tag Info |
C-terminal hFc-tagged |
| Form |
Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs |
Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | 3-7 business days |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |
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| Function |
Receptor for four distinct ligands: The TNF superfamily members TNFSF14/LIGHT and homotrimeric LTA/lymphotoxin-alpha and the immunoglobulin superfamily members BTLA and CD160, altogether defining a complex stimulatory and inhibitory signaling network. Signals via the TRAF2-TRAF3 E3 ligase pathway to promote immune cell survival and differentiation. Participates in bidirectional cell-cell contact signaling between antigen presenting cells and lymphocytes. In response to ligation of TNFSF14/LIGHT, delivers costimulatory signals to T cells, promoting cell proliferation and effector functions. Interacts with CD160 on NK cells, enhancing IFNG production and anti-tumor immune response. In the context of bacterial infection, acts as a signaling receptor on epithelial cells for CD160 from intraepithelial lymphocytes, triggering the production of antimicrobial proteins and proinflammatory cytokines. Upon binding to CD160 on activated CD4+ T cells, downregulates CD28 costimulatory signaling, restricting memory and alloantigen-specific immune response. May interact in cis (on the same cell) or in trans (on other cells) with BTLA. In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells.; (Microbial infection) Acts as a receptor for Herpes simplex virus 1/HHV-1.; (Microbial infection) Acts as a receptor for Herpes simplex virus 2/HHV-2.
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| Gene References into Functions |
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| Subcellular Location | Cell membrane; Single-pass type I membrane protein. |
| Tissue Specificity | Widely expressed, with the highest expression in lung, spleen and thymus. Expressed in a subpopulation of B cells and monocytes. Expressed in naive T cells. |
| Database Links |
HGNC: 11912 OMIM: 602746 KEGG: hsa:8764 STRING: 9606.ENSP00000347948 UniGene: Hs.512898 |
