Code | CSB-MP013481MOW |
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Size | $318 |
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Recombinant Macaca mulatta microtubule-associated protein tau (MAPT) is an active protein produced in mammalian cell lines. The gene encoding the 2-459aa of the Macaca mulatta MAPT was fused with the 10xHis-tag at the N-terminus and then cloned into the plasmid vector, finally transfected into the mammalian cells for subsequent expression. This recombinant MAPT protein was purified and reached over 95% in purity measured by SDS-PAGE. Its endotoxin is less than 1.0 EU/ug determined by the LAL method. Importantly, the activity of this protein has been validated in a functional ELISA in which it can bind the anti-MAPT recombinant antibody, with the EC50 of 2.464-3.979 ng/mL.
MAPT plays an important role in the occurrence and development of neuropathy-related diseases. The accumulation of hyperphosphorylated MAPT in neurons will cause neurofibrillary degeneration, which is associated with a variety of neurodegenerative diseases such as Alzheimer's disease (AD) and PD. Non-human primates (NHP) show high homology with humans in physiological function and drug metabolism. In particular, their brains are most similar to humans in structure and function. Therefore, scientists are hoping to be able to model human disease by building NHP models, especially in neuroscience. MAPT protein from Macaca mulatta goes beyond the limits of rodent studies.
Applications : Antigen
Review: I purchased lyophilized powder this time. After receiving the product, we dissolved it following the instructions, and it dissolved very well, and then through SDS detection, the purity was higher than the standard. After immunizing the mouse, coated the antigen, and several positive clones with good affinity were screened through ELISA. The antibody obtained from purified ascites has good specificity and high sensitivity. The overall performance of the product is good, the delivery is fast, and the COA report is very detailed!
By Anonymous