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Recombinant Macaca mulatta microtubule-associated protein tau (MAPT) is an active protein produced in mammalian cell lines. The gene encoding the 2-459aa of the Macaca mulatta MAPT was fused with the 10xHis-tag at the N-terminus and then cloned into the plasmid vector, finally transfected into the mammalian cells for subsequent expression. This recombinant MAPT protein was purified and reached over 95% in purity measured by SDS-PAGE. Its endotoxin is less than 1.0 EU/ug determined by the LAL method. Importantly, the activity of this protein has been validated in a functional ELISA in which it can bind the anti-MAPT recombinant antibody, with the EC50 of 2.464-3.979 ng/mL.
MAPT plays an important role in the occurrence and development of neuropathy-related diseases. The accumulation of hyperphosphorylated MAPT in neurons will cause neurofibrillary degeneration, which is associated with a variety of neurodegenerative diseases such as Alzheimer's disease (AD) and PD. Non-human primates (NHP) show high homology with humans in physiological function and drug metabolism. In particular, their brains are most similar to humans in structure and function. Therefore, scientists are hoping to be able to model human disease by building NHP models, especially in neuroscience. MAPT protein from Macaca mulatta goes beyond the limits of rodent studies.
|Purity||Greater than 95% as determined by SDS-PAGE.|
|Endotoxin||Less than 1.0 EU/ug as determined by LAL method.|
|Activity||Measured by its binding ability in a functional ELISA. Immobilized Macaca mulatta MAPT at 2 μg/mL can bind anti-MAPT recombinant antibody (CSB-RA013481A1HU),the EC50 is 2.464-3.979 ng/mL.|
(Neurofibrillary tangle protein) (Paired helical filament-tau) (PHF-tau)
|Species||Macaca mulatta (Rhesus macaque)|
|Target Protein Sequence||AEPRQEFDVMEDHAGTYGLGDRKDQEGYTMLQDQEGDTDAGLKESPLQTPAEDGSEELGSETSDAKSTPTAEDVTAPLVDERAPGEQAAAQPHMEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSATKQVQRKPPPAEPTSERGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPAREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL|
|Mol. Weight||50.6 kDa
|Protein Length||Full Length of Mature Protein|
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
|Buffer||Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0|
|Reconstitution||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.|
|Storage Condition||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.|
|Shelf Life||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
|Lead Time||Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.|
|Notes||Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.|
|Datasheet & COA||Please contact us to get it.|
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Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
|Gene References into Functions||
|Subcellular Location||Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell projection, axon. Cell projection, dendrite. Secreted.|
|Tissue Specificity||Expressed in neurons.|