AASS Antibody

Datasheet
Code CSB-PA883373LA01HU
Size US$299
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate
    All lanes: AASS antibody at 5.8µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 103 kDa
    Observed band size: 103 kDa

  • IHC image of CSB-PA883373LA01HU diluted at 1:400 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA883373LA01HU diluted at 1:400 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) AASS Polyclonal antibody
Uniprot No. Q9UDR5
Target Names AASS
Alternative Names AASSAlpha-aminoadipic semialdehyde synthase antibody; mitochondrial antibody; LKR/SDH) [Includes: Lysine ketoglutarate reductase antibody; LKR antibody; LOR antibody; EC 1.5.1.8); Saccharopine dehydrogenase antibody; SDH antibody; EC 1.5.1.9)] antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Alpha-aminoadipic semialdehyde synthase, mitochondrial protein (224-364AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:200-1:500
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Bifunctional enzyme that catalyzes the first two steps in lysine degradation. The N-terminal and the C-terminal contain lysine-ketoglutarate reductase and saccharopine dehydrogenase activity, respectively.
Gene References into Functions
  1. GR and KLF15 physically interact via low affinity GR binding sites within glucocorticoid response elements (GREs) for PRODH and AASS that contribute to combinatorial regulation with KLF15. PMID: 26088140
  2. Mitochondrial NADPH is crucial for AASS function PMID: 24847004
  3. Hyperlysinemia is caused by mutations in AASS PMID: 23570448
  4. Isolation and characterization of the mouse ortholog. PMID: 10567240
Involvement in disease Hyperlysinemia, 1 (HYPLYS1); 2,4-dienoyl-CoA reductase deficiency (DECRD)
Subcellular Location Mitochondrion
Protein Families AlaDH/PNT family; Saccharopine dehydrogenase family
Tissue Specificity Expressed in all 16 tissues examined with highest expression in the liver.
Database Links

HGNC: 17366

OMIM: 238700

KEGG: hsa:10157

STRING: 9606.ENSP00000377040

UniGene: Hs.156738

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