ADORA2A Antibody

Code CSB-PA007176
Size US$167
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Product Details

Uniprot No. P29274
Target Names ADORA2A
Alternative Names A2AAR antibody; A2aR antibody; AA2AR_HUMAN antibody; ADENO antibody; Adenosine A2 receptor antibody; Adenosine A2a receptor antibody; Adenosine receptor A2a antibody; Adenosine receptor subtype A2a antibody; ADORA 2 antibody; ADORA2 antibody; ADORA2A antibody; hA2aR antibody; RDC 8 antibody; RDC8 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Synthesized peptide derived from the Internal region of Human Adenosine A2A-R.
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Isotype IgG
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications WB, IHC, IF, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Protocols Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
(From Uniprot)
Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Gene References into Functions
  1. the A2AR-D2R heterotetramer-AC5 complex sustains the canonical antagonistic Gs-Gi interaction at the adenylyl cyclase level PMID: 29593213
  2. Here, ligand-binding kinetics of the full-length human adenosine A2A receptor (A2AR) reconstituted in detergent micelles were measured using a fluorescently labeled ligand via fluorescence anisotropy. PMID: 27577981
  3. alpha2A- and alpha2C-adrenoceptors are functional receptors for norepinephrine, dopamine, and other previously assumed selective D2-like receptor ligands. PMID: 29552726
  4. Mechanistic insights into allosteric regulation of the A2A adenosine G protein-coupled receptor by physiological cations have been described. PMID: 29636462
  5. expression A2AR mRNA in PBMCs was associated with asthma severity. Foxp3 mRNA, TGF-beta, and FEV1%pred positively correlated with A2AR mRNA in asthma. PMID: 27216911
  6. This study reported associations between the c.1083T>C polymorphism in the adenosine A2A receptor gene (ADORA2A) and sleep variables PMID: 28215251
  7. Enhancement of inosine-mediated A2AR signaling through positive allosteric modulation has been reported. PMID: 29126977
  8. These data indicate that low [Na(+)] is required to allow large agonist-induced structural changes in A2AR, and that patterns of sidechain dynamics substantially differ between agonist (NECA) and inverse agonist (ZM241385) bound receptors, with the inverse agonist suppressing fast picosecond-nanosecond timescale motions at the G protein binding site. PMID: 28984574
  9. Low adenosine A2a receptor expression is associated with ulcerative colitis. PMID: 27476546
  10. Results demonstrate that A2AR act on GR nuclear translocation and GR-dependent transcriptional regulation which suggest that A2AR is a major regulator of GR function and that this functional interconnection may be a trigger to age-related memory deficits. PMID: 27510168
  11. the present study indicates that A2bR may play a potential oncogenic role in BUC progression and act as a potential biomarker to identify BUC patients with poor clinical outcomes. PMID: 28548944
  12. Report prognostic impact of ADORA2A expression in non-small lung cancers. PMID: 28060732
  13. genetic association studies in population in Germany: Data suggest that an SNP in ADORA2A (1976T/C, rs5751876) is associated with interoceptive (resting) and exteroceptive (executive function/task-based) processing in frontal lobe and insular cortex neural networks. Facilitated processing of interoceptive/exteroceptive information in salience network is suggested to promote development of anxiety and anxiety disorders. PMID: 27262510
  14. A2A receptor stimulation promotes collagen type III synthesis via the activation of canonical and non-canonical beta-catenin, consistent with a role for A2A receptors in dermal fibrosis and scarring. PMID: 27595240
  15. It is significantly associated with hippocampal volume and the minor allele of rs9608282 in ADORA2A is associated with larger hippocampal volumes and better memory. PMID: 28941407
  16. Study corroborated results from a previous report that described interactions between ADORA2A and CYP1A2 polymorphisms and coffee consumption in Parkinson's disease. PMID: 28135712
  17. However, the contribution of the A2AR to the control of impulsive reward seeking remains unknown. Using mice that were exposed to differential reward of low rate (DRL) schedules during Pavlovian-conditioning, second-order schedule discrimination, and the 5-choice serial reaction time task (5-CSRTT), we demonstrate that deficits of A2AR function promote impulsive responses. PMID: 28418405
  18. Western blot analysis indicates that the A2A receptor is more abundant in the hippocampus of medial temporal lobe epilepsy patients compared to control individuals. Immunoreactivity against the A2A receptor predominates in astrocytes staining positively for the glial fibrillary acidic protein. PMID: 27650530
  19. Results suggest that the combination of the ADORA2A rs2298383TT and MTHFR 1298AC-677CT genotypes might lead to a low risk of nodule formation in patients treated with methotrexate. PMID: 27846189
  20. Hypoxic postconditioning protects against apoptosis induced by reoxygenation via activation of adenosine A2a receptors on dermal microvascular endothelial cells. PMID: 28606618
  21. results unveil intracellular signaling pathways targeted by the A2AR, some of which might be key in modulating neutrophil functions. PMID: 28179537
  22. A2A AR activation on BBB endothelial cells offers a therapeutic window that can be fine-tuned for drug delivery to the brain and has potential as a CNS drug-delivery technology. PMID: 27043281
  23. Hypoxic postconditioning attenuates apoptosis via inactivation of adenosine A2a receptor through NDRG3-Raf-ERK pathway. PMID: 28743501
  24. ADORA2A rs2298383 and high cumulative dose of systemic methotrexate (MTX) administration were significantly associated with MTX-related leukoencephalopathy PMID: 27399166
  25. lipid 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC, di-6:0PC). After solubilization in DDM, DDM/CHAPS, or DHPC micelles, although A2AR was found to retain its native-like fold, its binding ability was significantly compromised compared to DDM or DDM/CHAPS with CHS. It therefore appears that although cholesterol is not needed for A2AR to retain a native-like, alpha-helical conformation PMID: 27241126
  26. We report an investigation designed to explore alternative approaches for ranking of docking poses in the search for antagonists of the adenosine A2A receptor, an attractive target for structure-based virtual screening PMID: 27334985
  27. A higher regeneration rate in the CF102 treatment group was observed compared with the control group, suggesting that CF102 had a positive effect on the proliferation of hepatocytes following hepatectomy. CF102 had a protective effect on the liver of Wistar rats subsequent to IR injury during hepatectomy. This may be due to an antiinflammatory and antiapoptotic effect mediated by the A3AR. PMID: 27666664
  28. Adenosine A(2A) receptor 1083 T > C polymorphism is not associated with the positivity of head-up tilt test and its proposed role in predisposition to Vasovagal Syncope was not confirmed PMID: 26710963
  29. Low level of ADORA1/ADORA2A expression increased susceptibility of brain tumor-associated epilepsy. PMID: 27038930
  30. No association was found between the ADORA2A rs2298383 polymorphism and panic disorder. The ADORA2A TT homozygous wild-type genotype and the ACCN2 TT/ADORA2A CT diplotype were more represented in controls. PMID: 26589317
  31. gene expression of A2AR in PBMCs was significantly lower in idiopathic normal-pressure hydrocephalus patients than controls PMID: 26810542
  32. crystal structure of the adenosine A(2A) receptor bound to an engineered G protein at 3.4 A resolution PMID: 27462812
  33. This review will provide insight into the phenotypic changes that underline the resistance to death of liver cells preconditioned by pharmacological activation of A2aR and their implications to develop innovative strategies against liver IR damage PMID: 26539478
  34. Data suggest that A2aR binding of antagonist (here, ZM241385) exhibits multistep drug-dissociation process, with antagonist consecutively interacting with topographically distinct regions/binding sites of A2aR. PMID: 26873858
  35. activation of the A2AR reduced oxidative stress-induced DNA damage and caspase 3 activation in GABAergic neurons PMID: 26264576
  36. Functional polymorphisms of the genes encoding adenosine A2A receptors and dopamine transporters contribute to individual differences in impaired sleep quality by caffeine. PMID: 27167478
  37. This review examines the molecular mechanisms of inflammatory response in sepsis and the structure and functions of A(2A)AR and its role in the pathogenesis of sepsis PMID: 27239843
  38. (19)F nuclear magnetic resonance is used to quantify the conformational landscape occupied by the adenosine A2A receptor (A2AR), a prototypical class A G-protein-coupled receptor PMID: 27144352
  39. ST1535 metabolites ST3932 and ST4206 are effective adenosine A2A receptor antagonists which maybe useful in treating Parkinson disease. PMID: 25936513
  40. activation of A2A adenosine receptor (AR) with an FDA-approved agonist potently permeabilizes an in vitro primary human blood-brain barrier (hBBB) to the passage of chemotherapeutic drugs and T cells. PMID: 25262373
  41. ADORA2A and GRK5 gene variants may influence the etiology of malaria infection. PMID: 26066465
  42. placental expression highest at no more than 10 weeks gestational age PMID: 25745823
  43. a comparative molecular dynamics (MD) study of the human adenosine receptor type 2A (hA(2A)R) in complex with caffeine, is reported. PMID: 25992797
  44. these data suggest a possible role for CD73 and A2A in inflammation observed in patients with T2D and obesity mediated via apoptosis. PMID: 25770019
  45. Genetic study showed association of single nucleotide polymorphisms in alpha2A-AR gene locus with bone remodelling markers, identifying the individuals with higher risk of development of osteoporosis. PMID: 25818344
  46. This study found a relation between polymorphisms of adenosine A2A and the caffeine effects on the attentional domains of Orienting and Executive control. PMID: 25819143
  47. Expression of NPP1 and 5'-nucleotidase by valve interstitial cells promotes the mineralization of the aortic valve through A2aR and a cAMP/PKA/CREB pathway. PMID: 25644539
  48. An association was observed for SNPs in ADORA2A and levodopa-induced dyskinesia in Parkinson's disease patients. PMID: 25872644
  49. the A2AR-SMALP, generated from yeast (Pichia pastoris) or mammalian cells, exhibited increased thermostability (~5 degrees C) compared with detergent [DDM (n-dodecyl-beta-D-maltopyranoside)]-solubilized A2AR controls. PMID: 25720391
  50. findings provide new insights into the anti-inflammatory mechanisms of A2AAR signaling and the potential utility of A2AAR agonists in inflammatory diseases. PMID: 26355151

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Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Database Links

HGNC: 263

OMIM: 102776

KEGG: hsa:135

STRING: 9606.ENSP00000336630

UniGene: Hs.197029

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