AIFM2 Antibody

Datasheet

Citations (1)

Code	CSB-PA874794LA01HU
Size	US$166
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Image	IHC image of CSB-PA874794LA01HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Western Blot Positive WB detected in: A549 whole cell lysate All lanes: AIFM2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41, 37 kDa Observed band size: 41 kDa Immunofluorescence staining of HepG2 cells with CSB-PA874794LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
Citations
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Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) AIFM2 Polyclonal antibody

Uniprot No.

Q9BRQ8

Target Names

AIFM2

Alternative Names

5430437E11Rik antibody; aifm2 antibody; AIFM2_HUMAN antibody; AMID antibody; Apoptosis inducing factor (AIF) homologous mitochondrion associated inducer of death antibody; Apoptosis inducing factor (AIF) like mitochondrion associated inducer of death antibody; Apoptosis inducing factor mitochondrion associated 2 antibody; Apoptosis-inducing factor 2 antibody; Apoptosis-inducing factor homologous mitochondrion-associated inducer of death antibody; Apoptosis-inducing factor-like mitochondrion-associated inducer of death antibody; Cys51Stop antibody; HGNC11998 antibody; p53 responsive gene 3 antibody; p53 tumor suppressor antibody; p53-responsive gene 3 protein antibody; PRG3 antibody; TRP53 antibody; Tumor protein p53 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Apoptosis-inducing factor 2 protein (110-373AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The AIFM2 Antibody (Product code: CSB-PA874794LA01HU) is Non-conjugated. For AIFM2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA874794LB01HU	AIFM2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA874794LC01HU	AIFM2 Antibody, FITC conjugated
Biotin	CSB-PA874794LD01HU	AIFM2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

Long non-coding RNA TUG1 induces ferroptosis in hippocampal neurons and depressive-like behaviors by facilitating the ubiquitination of DUSP14 Y Yang,/,2024

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : Western blotting(WB)

Sample type: Tissues

Review: The protein expression levels of Aifm2.

By Anonymous

Target Background

Function

A NAD(P)H-dependent oxidoreductase involved in cellular oxidative stress response. At the plasma membrane, catalyzes reduction of coenzyme Q/ubiquinone-10 to ubiquinol-10, a lipophilic radical-trapping antioxidant that prevents lipid oxidative damage and consequently ferroptosis. Cooperates with GPX4 to suppress phospholipid peroxidation and ferroptosis. This anti-ferroptotic function is independent of cellular glutathione levels. May play a role in mitochondrial stress signaling. Upon oxidative stress, associates with the lipid peroxidation end product 4-hydroxy-2-nonenal (HNE) forming a lipid adduct devoid of oxidoreductase activity, which then translocates from mitochondria into the nucleus triggering DNA damage and cell death. Capable of DNA binding in a non-sequence specific way.

Gene References into Functions

Low AIFM2 expression is associated with T-cell lymphoblastic lymphoma. PMID: 28534937
AIF and its family member protein, AMID, are rotenone-sensitive NADH:ubiquinone oxidoreductases (of the NDH-2 type). PMID: 26063804
HUHS1015 increased nuclear localization of apoptosis-inducing factor-homologous mitochondrion-associated inducer of death (AMID). PMID: 25244912
induces caspase-independent apoptosis PMID: 11980907
encodes a homologue of the apoptosis-inducing factor PMID: 12135761
AMID is a p53-downstream gene involved in tumorigenesis. PMID: 15273740
Reasults establish a link between coenzyme and DNA binding that likely impacts on the physiological role of AMID in cellular apoptosis. PMID: 15958387
study of cellular localization of the endonuclease G, AIF & AMID during apoptosis using bioinformatics and image analysis PMID: 17347867
AIF-M2 lessens survival cell signaling in the presence of foreign (e.g. bacterial and (retro)viral) cytosolic DNA, thus contributing to the onset of apoptosis PMID: 17711848

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Subcellular Location

Lipid droplet. Cell membrane; Lipid-anchor. Cytoplasm. Mitochondrion membrane. Nucleus.

Protein Families

FAD-dependent oxidoreductase family

Tissue Specificity

Detected in most normal tissues as two transcripts of 1.8 and 4.0 kb in length, respectively. Highly expressed in heart, moderately in liver and skeletal muscles, and expressed at low levels in placenta, lung, kidney, and pancreas. Both transcripts expres

Database Links

HGNC: 21411

OMIM: 605159

KEGG: hsa:84883

STRING: 9606.ENSP00000312370

UniGene: Hs.650680

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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