ALG9 Antibody

Datasheet

Code	CSB-PA867161LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate All lanes: ALG9 antibody at 6.3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 70, 51, 71, 52 kDa Observed band size: 70 kDa IHC image of CSB-PA867161LA01HU diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ALG9 Polyclonal antibody

Uniprot No.

Q9H6U8

Target Names

ALG9

Alternative Names

ALG9 antibody; ALG9_HUMAN antibody; Alpha-1,2-mannosyltransferase ALG9 antibody; Asparagine-linked glycosylation protein 9 homolog antibody; Disrupted in bipolar disorder protein 1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Alpha-1,2-mannosyltransferase ALG9 protein (434-618AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ALG9 Antibody (Product code: CSB-PA867161LA01HU) is Non-conjugated. For ALG9 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA867161LB01HU	ALG9 Antibody, HRP conjugated	ELISA
FITC	CSB-PA867161LC01HU	ALG9 Antibody, FITC conjugated
Biotin	CSB-PA867161LD01HU	ALG9 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Catalyzes the transfer of mannose from Dol-P-Man to lipid-linked oligosaccharides.

Gene References into Functions

ALG9 is upregulated in peripheral blood mononuclear cells of galactosaemia patients. PMID: 26733289
Our study shows that some pathogenic variants in ALG9 can present as a lethal skeletal dysplasia with visceral malformations as the most severe phenotype PMID: 25966638
Due to the ALG9 deficiency, cells accumulated the lipid-linked oligosaccharides Man(6)GlcNAc(2)-PP-dolichol and Man(8)GlcNAc(2)-PP-dolichol. PMID: 19451548

Involvement in disease

Congenital disorder of glycosylation 1L (CDG1L); Gillessen-Kaesbach-Nishimura syndrome (GIKANIS)

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Families

Glycosyltransferase 22 family

Tissue Specificity

Ubiquitously expressed; with highest levels in heart, liver and pancreas.

Database Links

HGNC: 15672

OMIM: 263210

KEGG: hsa:79796

STRING: 9606.ENSP00000435517

UniGene: Hs.745155

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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