APAF1 Antibody

Datasheet
Code CSB-PA257161
Size US$299
Uniprot No. O14727
Image
  • The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA257161(APAF1 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)
  • The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA257161(APAF1 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)
Immunogen Synthetic peptide of Human APAF1
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Tested Applications ELISA,IHC;ELISA:1:1000-1:10000,IHC:1:25-1:100
Relevance This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
Form Liquid
Conjugate Non-conjugated
Storage Buffer -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method Antigen affinity purification
Isotype IgG
Alias apoptotic peptidase activating factor 1
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Target Names APAF1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Subcellular Location Cytoplasm
Tissue Specificity Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
Database Links

HGNC: 576

OMIM: 602233

KEGG: hsa:317

STRING: 9606.ENSP00000448165

UniGene: Hs.552567

Pathway p53 signaling pathway
Apoptosis

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