Product Details

Purity

≥ 90% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human APOE at 2 μg/mL can bind Anti-APOE recombinant antibody (CSB-RA001936MA1HU). The EC50 is 2.574-2.908 ng/mL.

Target Names

APOE

Uniprot No.

P02649

Alternative Names

Apolipoprotein E; Apo-E; APOE

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

19-317aa

Target Protein Sequence

KVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH

Mol. Weight

37.0 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Apolipoprotein E mediates lipid transport and cholesterol homeostasis in the central nervous system, making it a critical target for investigating neurodegenerative disease mechanisms where lipid metabolism is disrupted. This full-length mature protein (aa 19–317) demonstrates quantifiable binding activity with an EC50 of 2.574–2.908 ng/mL when tested against a recombinant anti-APOE antibody in functional ELISA, providing researchers with validated material for antibody screening, epitope mapping studies, and binding kinetics experiments. Mammalian cell expression preserves native post-translational modifications essential for authentic lipid-binding behavior and receptor interactions. The protein achieves greater than 90% purity by SDS-PAGE and contains less than 1.0 EU/μg endotoxin, meeting the quality thresholds commonly required for cell-based lipid uptake assays and in vitro receptor binding studies where low endotoxin levels prevent interference with cellular responses.

Target Background

Function

APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apoliproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. Finally, APOE has also a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells. A main function of APOE is to mediate lipoprotein clearance through the uptake of chylomicrons, VLDLs, and HDLs by hepatocytes. APOE is also involved in the biosynthesis by the liver of VLDLs as well as their uptake by peripheral tissues ensuring the delivery of triglycerides and energy storage in muscle, heart and adipose tissues. By participating in the lipoprotein-mediated distribution of lipids among tissues, APOE plays a critical role in plasma and tissues lipid homeostasis. APOE is also involved in two steps of reverse cholesterol transport, the HDLs-mediated transport of cholesterol from peripheral tissues to the liver, and thereby plays an important role in cholesterol homeostasis. First, it is functionally associated with ABCA1 in the biogenesis of HDLs in tissues. Second, it is enriched in circulating HDLs and mediates their uptake by hepatocytes. APOE also plays an important role in lipid transport in the central nervous system, regulating neuron survival and sprouting. APOE is also involved in innate and adaptive immune responses, controlling for instance the survival of myeloid-derived suppressor cells. APOE, may also play a role in transcription regulation through a receptor-dependent and cholesterol-independent mechanism, that activates MAP3K12 and a non-canonical MAPK signal transduction pathway that results in enhanced AP-1-mediated transcription of APP.; (Microbial infection) Through its interaction with HCV envelope glycoprotein E2, participates in the attachment of HCV to HSPGs and other receptors (LDLr, VLDLr, and SR-B1) on the cell surface and to the assembly, maturation and infectivity of HCV viral particles. This interaction is probably promoted via the up-regulation of cellular autophagy by the virus.

Gene References into Functions

Patients having Glutathione S-transferase M1 allele and Apolipoprotein E E2 allele are predisposed to oxidative stress-induced cardiac injury. PMID: 30095041
the APOEepsilon4 alleles were significantly higher in patients with Alzheimer's disease (AD) and there was a potential synergistic interaction between the CYP46A1 C allele and APOEepsilon4 allele in AD. PMID: 29516283
familial hypercholesterolemia case-control study was associated with the E4 allele in the Saudi population. However, E4 allele was appeared as a reliable risk marker for lipid profiles, but not for ApoE alleles PMID: 30235358
Significant interactions between APOE epsilon4 and mild cognitive impairment were found in general cognitive function (p = 0.001) and white matter connectivity network (clustering coefficient, p = 0.004, and local efficiency. PMID: 27785756
data presented here could increase the knowledge of ApoE3 activity and be a starting point for the study of the impact of variations on APOE gene PMID: 28559539
APOE-epsilon4 is associated with a subtly faster rate of memory decline from midlife to early old age PMID: 29317609
This study aimed to assess the association between APOE genotype and saccade latency using a prosaccade and antisaccade task in young individuals (N=97, age: 17-35 years). Results showed that prosaccade latency was significantly delayed in a group of epsilon4 carriers in comparison to non-carriers, which was due to a lower rate of signal accumulation rather than a change in the criterion threshold. PMID: 30003976
Higher plasma ApoE concentrations (> 5.9 mg/dL) are the independent risk factor for hemorrhage during endobronchial biopsy in patients with lung cancer. PMID: 30031394
Apolipoprotein E Mutation is associated with Lipoprotein Glomerulopathy. PMID: 29398675
these results suggest a susceptibility of E4+ carriers to metabolic and cognitive impairments brought on by a HFD, and may help guide development of novel therapeutic targets for AD and related dementias. PMID: 28272510
A randomized trial and novel SPR technique identifies altered lipoprotein-LDL receptor binding as a mechanism underlying elevated LDL-cholesterol in APOE4s PMID: 28276521
Site-directed mutagenesis of Akt at Cys224 revealed that S-nitrosylation at this site was pivotal for the reduced phosphorylation at Akt Ser473, which led to impaired Akt signaling. Furthermore, on HHcy challenge, as compared with GSNOR(+/+)ApoE(-/-) littermate controls, GSNOR(-/-)ApoE(-/-) double knockout mice showed reduced T-cell activation with concurrent reduction of atherosclerosis. PMID: 29860106
U.S. military veterans who are APOE epsilon4 allele carriers and exposed to a high number of traumas may be at increased risk for developing PTSD symptoms than epsilon4 noncarriers. PMID: 29172227
found that the ApoE genotype was significantly associated with aneurysmal subarachnoid hemorrhage risk, whereas its effect on certain ethnic populations differs PMID: 30010064
APOE polymorphism might be associated with the incidence of aneurysmal subarachnoid hemorrhage in Chinese Fujian Han population. PMID: 29769126
Reduced expression of miR-98 may relate to LOX-1 expression and foam cell formation and lipid accumulation in aortas of ApoE(-/-) mice. PMID: 29549823
The epsilon4+ subjects exhibited lower inter- and intrahemispheric coherences than the epsilon4- individuals. CONCLUSION: epsilon4 carriers have lower corticocortical communication than noncarriers during an intelligence task, implying that carrying the epsilon4 allele may reduce brain networking in adolescence, several decades before the onset of Alzheimer disease. PMID: 28848214
APOE-epsilon4 modified the association between central arterial stiffening and cognition. PMID: 29656011
The rs7755 and rs3211956 loci polymorphisms of CD36 gene and genotype E2/E3, E3/E4, E4/E4 of ApoE gene, and E2 and E4 alleles were statistically related with Alzheimer disease. PMID: 30235742
results show great changes in men carrying ApoE2, mainly in TG and TC concentrations after treatment with hypocaloric diet and controlled exercise PMID: 29756962
ApoE genetic polymorphisms conferred susceptibility to steroid-induced ONFH in Chinese Han population. PMID: 29892926
t results point out that APOE4 genotype influences healthy aged cognition, although factors such age or educational attainment seem to modulate its effects PMID: 29414814
Reduced content of ApoE in blood serum of men with arterial hypertension was associated with APOE4 gene polymorphism. PMID: 29658078
In summary, our findings from the two cohorts confirm that genetic variations at the APOE locus influence plasma total cholesterol concentrations, however, the gene-diet interactions on lipids require further investigation in larger cohorts. PMID: 29712557
There is the association between ApoE polymorphism and hypertension. [meta-analysis] PMID: 30180966
The authors propose that APOE4-driven hyperactivity may be a causative factor driving increased risk of Alzheimer's disease among APOE4 carriers. PMID: 29133888
Our results showed that ApoE epsilon4 and PON1-55M alleles act synergistically to increase the risk of systemic lupus erythematosus by 1.47 times PMID: 29273831
Study presents evidence that odor identification ability starts to decline already in middle-aged, and that carriers of varepsilon4/4, who are at highest risk of developing dementia, decline twice as fast. PMID: 28455505
Findings suggest that the apolipoprotein E4 genotype selectively modulates the functional integration of brain networks in patients with amnestic mild cognitive impairment, thus providing important insight into the gene-connectome interaction in this disease. PMID: 28341847
Findings revealed that APOE epsilon4 allele has a detrimental effect on parietal and frontal white matter in cognitively normal elderly; and demonstrate that information processing speed deficits can be explained by parietal white matter hyperintensities volume, age and interaction between parietal white matter hyperintensities volume and age. PMID: 27444731
The study demonstrated that the APOE epsilon3/epsilon3 and epsilon3/epsilon4 genotypes played a protective role against cerebral infarction in Chinese men, but not women. PMID: 29479056
corroborating evidence that supports the fact that the presence of the APOE e4 polymorphism confers greater susceptibility to combat-related post-traumatic stress disorder (meta-analysis)> PMID: 28430712
Carriers of the 4 allele of the APOE genotype had significantly lower LDL particle size and LDL I proportion compared to 33 homozygotes ( P < .05 for both comparisons). Insulin resistance increased from patients with no OSA to those with mild-moderate and to those with severe OSA ( P < .001). In multivariate analysis, LDL size was independently predicted by APOE genotype. PMID: 26941371
In het mice, RIF had no effect on plasma lipids composition, CYP27A1 activity, and atherosclerotic plaque development, despite a reduction in cholesterol absorption. In conclusion, the antiatherogenic effect of Cyp3a11 induction by RIF was also dependent on Cyp27a1 expression. PMID: 29191818
Hypertension and the ApoEepsilon4/epsilon4 genotype may be associated with the occurrence of MCA stenosis in the ischemic stroke Chinese patients. PMID: 29494434
In this study, detection of AD-related DNA oligonucleotide sequence associated with Apolipoprotein E4 gene sequence was achieved using localized-surface plasmon resonance (LSPR) on 2D-Photonic crystal (2D-PC) and Au-coated 2D-PC surfaces. PMID: 29291596
APOE polymorphism has an influence on the risk of aneurysmal subarachnoid hemorrhage. PMID: 29213291
Evidence for an interaction between APOE SNP rs1064725 and dietary fat intake on fasting total plasma cholesterol concentrations. PMID: 29169396
APOE-epsilon4 carriers had elevated carotid intima media thickness independent of demographics and vascular risk factors. Diabetes was an effect modifier of the relationship between APOE-epsilon4 and cIMT. PMID: 29103864
APOE is a risk gene for ischemic stroke (IS), but not independent, especially for large artery atherosclerosis IS. PMID: 29074556
Among APOE4 carriers, race was not associated with risk of Alzheimer's disease. PMID: 28863046
association of rare variants near the APOE region with CSF and neuroimaging biomarkers of Alzheimer's disease PMID: 28589856
The APOE-epsilon4 allele may confer an increased risk of impairment of 6-month verbal memory for patients suffering mild traumatic brain injury. PMID: 28948085
The APOE e4 allele is a risk factor for late onset Alzheimer's disease as well as for other types of dementia.Living arrangements, perceived social support, and loneliness were found to moderate the relationship between the APOE e4 allele and cognitive function. PMID: 28329797
epsilon2 carriers perform better on cognitive tests as this group have a lower risk of Alzheimer's disease. PMID: 29032015
Overnight hypoxemia and sleepiness were associated with cognition. The average effect estimates were small, similar to effect estimates for several other individual dementia risk factors. Associations were strongest in APOE-epsilon4 risk allele carriers. PMID: 28731362
Downregulation of apoE and apoJ in CSF strongly suggests a critical role of lipid metabolism in the development and progression of Moyamoya disease. PMID: 28843803
ApoE epsilon4 alleles are associated with dementia risk. PMID: 28657841
Findings suggest a possible relationship between the epsilon4 allele and post-concussion impairment, as well as between the epsilon4 allele and neurocognitive performance variability, suggesting that the epsilon4 genotype may be a risk factor for less efficient cognitive processing in concussed athletes. PMID: 28541413
APOE4 carriers and the CETP genotype were associated with a decreased response to simvastatin therapy PMID: 28851085

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Involvement in disease

Hyperlipoproteinemia 3 (HLPP3); Alzheimer disease 2 (AD2); Sea-blue histiocyte disease (SBHD); Lipoprotein glomerulopathy (LPG); Familial hypercholesterolemia (FH)

Subcellular Location

Secreted. Secreted, extracellular space. Secreted, extracellular space, extracellular matrix.

Protein Families

Apolipoprotein A1/A4/E family

Tissue Specificity

Produced by several tissues and cell types and mainly found associated with lipid particles in the plasma, the interstitial fluid and lymph. Mainly synthesized by liver hepatocytes. Significant quantities are also produced in brain, mainly by astrocytes a

Database Links

HGNC: 613

OMIM: 104310

KEGG: hsa:348

STRING: 9606.ENSP00000252486

UniGene: Hs.654439

Code	CSB-MP001936HU
Abbreviation	Recombinant Human APOE protein (Active)
MSDS	MSDS Datasheet
Size	$9.9
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Human APOE at 2 μg/ml can bind Anti-APOE recombinant antibody (CSB-RA001936MA1HU). The EC₅₀ is 2.574-2.908 ng/mL. Biological Activity Assay
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Recombinant Human Apolipoprotein E (APOE) (Active)

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