Code | CSB-PA002288HA01HU |
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US$299Purchase it in Cusabio online store (only available for customers from the US) |
Uniprot No. | Q9NT62 |
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Immunogen | Recombinant Human Ubiquitin-like-conjugating enzyme ATG3 protein (1-314AA) |
Raised in | Rabbit |
Species Reactivity | Human |
Tested Applications | ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:1000-1:2000, IF:1:50-1:500 |
Relevance | E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. |
Form | Liquid |
Conjugate | Non-conjugated |
Storage Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method | >95%, Protein G purified |
Isotype | IgG |
Clonality | Polyclonal |
Alias | Ubiquitin-like-conjugating enzyme ATG3 (EC 2.3.2.-) (Autophagy-related protein 3) (APG3-like) (hApg3) (Protein PC3-96), ATG3, APG3 APG3L |
Immunogen Species | Homo sapiens (Human) |
Protocols | ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol |
Research Area | Cell Biology |
Target Names | ATG3 |
Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
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Function | E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. |
Subcellular Location | Cytoplasm |
Protein Families | ATG3 family |
Tissue Specificity | Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. |
Database Links |
HGNC: 20962 OMIM: 609606 KEGG: hsa:64422 STRING: 9606.ENSP00000283290 UniGene: Hs.477126 |
Pathway | Autophagy |
ATG3 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA002288GA01HU | ATG3 Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA002288HB01HU | ATG3 Antibody, HRP conjugated | Human | ELISA |
CSB-PA002288HC01HU | ATG3 Antibody, FITC conjugated | Human | ELISA |
CSB-PA002288HD01HU | ATG3 Antibody, Biotin conjugated | Human | ELISA |
CSB-PA914063 | ATG3 Antibody | Human,Mouse,Rat | ELISA,IHC |
CSB-PA777675 | ATG3 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
ATG3 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP889107HU CSB-EP889107HU CSB-BP889107HU CSB-MP889107HU |
Recombinant Human Ubiquitin-like-conjugating enzyme ATG3(ATG3) | Yeast E.coli Baculovirus Mammalian cell |
CSB-EP002288HU | Recombinant Human Ubiquitin-like-conjugating enzyme ATG3(ATG3) | E.coli |
CSB-YP002288HU CSB-BP002288HU CSB-MP002288HU |
Recombinant human Ubiquitin-like-conjugating enzyme ATG3 | Yeast Baculovirus Mammalian cell |
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