ATL2 Antibody

Datasheet

Code	CSB-PA836776LA01HU
Size	$600
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Image	Western Blot Positive WB detected in: MCF7 whole cell lysate, HL60 whole cell lysate, HEK293 whole cell lysate, Hela whole cell lysate All lanes: ATL2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 67 kDa Observed band size: 67 kDa Immunofluorescence staining of HepG2 cell with CSB-PA836776LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ATL2 Polyclonal antibody

Uniprot No.

Q8NHH9

Target Names

ATL2

Alternative Names

ATL2; ARL6IP2; Atlastin-2; ADP-ribosylation factor-like protein 6-interacting protein 2; ARL-6-interacting protein 2; Aip-2

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Atlastin-2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ATL2 Antibody (Product code: CSB-PA836776LA01HU) is Non-conjugated. For ATL2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA836776LB01HU	ATL2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA836776LC01HU	ATL2 Antibody, FITC conjugated
Biotin	CSB-PA836776LD01HU	ATL2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.02% sodium azide
Constituents: PBS containing 50% glycerol pH 7.3

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:4000
IF	1:20-1:100

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

GTPase tethering membranes through formation of trans-homooligomers and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis.

Gene References into Functions

These results suggest that the three ATLs have different capacities to mediate endoplasmic reticulum fusion, with ATL1 being the strongest and ATL3 being the weakest. PMID: 25773277
The role of GTP hydrolysis in the ATL2 fusion mechanism also needs to be tempered by the uncertainty of whether the behavior of the ATL2 soluble domain, observed herein, reflects the behavior of the full-length, membrane-anchored protein. PMID: 22065636
A family of human GTPases, atlastin-2 and -3 that are closely related to atlastin-1 are described. PMID: 18270207

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Families

TRAFAC class dynamin-like GTPase superfamily, GB1/RHD3-type GTPase family, GB1 subfamily

Tissue Specificity

Expressed in peripheral tissues (at protein level).

Database Links

HGNC: 24047

OMIM: 609368

KEGG: hsa:64225

STRING: 9606.ENSP00000368237

UniGene: Hs.594950

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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