CA2 Antibody

Datasheet
Code CSB-PA004370HA01HU
See More Details Free Antibody trial simple
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. P00918
Image
  • Western Blot
    Positive WB detected in: Rat kidney tissue, Rat heart tissue, Rat liver tissue, Mouse lung tissue, Mouse kidney tissue
    All lanes: CA2 antibody at 2µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 30 kDa
    Observed band size: 30 kDa

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA004370HA01HU at dilution of 1:100

  • Immunofluorescent analysis of Hela cells using CSB-PA004370HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • IHC image of CSB-PA004370HA01HU diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA004370HA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human Carbonic anhydrase 2 protein (2-260AA)
Raised in Rabbit
Species Reactivity Human, Mouse, Rat
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:500, IF:1:50-1:200
Relevance Essential for bone resorption and osteoclast differentiation .Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Carbonic anhydrase 2 (EC 4.2.1.1) (Carbonate dehydratase II) (Carbonic anhydrase C) (CAC) (Carbonic anhydrase II) (CA-II), CA2
Immunogen Species Human
Research Area Cardiovascular
Target Names CA2
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
References
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Function Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6.
Involvement in disease Osteopetrosis, autosomal recessive 3 (OPTB3)
Subcellular Location Cytoplasm, Cell membrane
Protein Families Alpha-carbonic anhydrase family
Database Links

HGNC: 1373

OMIM: 259730

KEGG: hsa:760

STRING: 9606.ENSP00000285379

UniGene: Hs.155097

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