CLTCL1 Antibody

Datasheet

Code	CSB-PA005594LA01HU
Size	US$166
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Image	Western blot All lanes: CLTCL1 antibody at 2µg/ml Lane 1: Jurkat whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lane 3: A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 188, 181 kDa Observed band size: 188 kDa Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA005594LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA005594LA01HU at dilution of 1:100 Immunofluorescence staining of A549 cells with CSB-PA005594LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CLTCL1 Polyclonal antibody

Uniprot No.

P53675

Target Names

CLTCL1

Alternative Names

CLTCL1 antibody; CLH22 antibody; CLTCL antibody; CLTDClathrin heavy chain 2 antibody; Clathrin heavy chain on chromosome 22 antibody; CLH-22 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Clathrin heavy chain 2 protein (1423-1566AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CLTCL1 Antibody (Product code: CSB-PA005594LA01HU) is Non-conjugated. For CLTCL1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA005594LB01HU	CLTCL1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA005594LC01HU	CLTCL1 Antibody, FITC conjugated
Biotin	CSB-PA005594LD01HU	CLTCL1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.

Gene References into Functions

Provide biochemical evidence for separate regulation and distinct functional niches for CHC17 and CHC22 in HeLa cells. Furthermore, the greater stability of the CHC22 coat relative to the CHC17 coat may be relevant to its excessive accumulation with GLUT4 during insulin resistance. PMID: 29097553
Clathrin heavy chain 22 contributes to the control of neuropeptide degradation and secretion during neuronal development. Study suggests This suggests that a reduction in CHC22 expression in sensory neural precursors may license a step in neuron precursor neurodevelopment; and that this step is mediated through control of a novel neuropeptide processing pathway. PMID: 29402896
CLTCL1 is significantly upregulated in the developing human brain PMID: 26068709
Depletion of clathrin heavy chain (CHC)17, but not the CHC22 clathrin isoform, by ribonucleic acid interference (RNAi) induces centrosome amplification and multipolar spindles. PMID: 22891263
CHC22 was required for retrograde trafficking of certain cargo molecules from endosomes to the trans-Golgi network. PMID: 20065094
Clathrin isoform CHC22 binds to sorting nexin 5 through a coiled-coil domain. PMID: 15133132
role for CHC22 in formation of insulin-responsive GLUT4 compartments in muscle & adipocytes; CHC22 associated with expanded GLUT4 compartments in muscle in type 2 diabetes PMID: 19478182

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Subcellular Location

Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side.

Protein Families

Clathrin heavy chain family

Tissue Specificity

Maximal levels in skeletal muscle. High levels in heart and testis. Low expression detected in all other tissues.

Database Links

HGNC: 2093

OMIM: 601273

KEGG: hsa:8218

STRING: 9606.ENSP00000445677

UniGene: Hs.368266

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The Overview of Organelles Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Endocytosis Synaptic vesicle cycle

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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