CYB5B Antibody

Code CSB-PA006310LA01HU
Size US$166
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  • Western blot
    All lanes: CYB5B antibody at 0.8µg/ml
    Lane 1: HepG2 whole cell lysate
    Lane 2: Mouse liver tissue
    Lane 3: Mouse heart tissue
    Lane 4: Hela whole cell lysate
    Lane 5: U87 whole cell lysate
    Lane 6: EC109 whole cell lysate
    Lane 7: A549 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA006310LA01HU at dilution of 1:100

  • Immunofluorescent analysis of HepG2 cells using CSB-PA006310LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) CYB5B Polyclonal antibody
Uniprot No.
Target Names
CYB5B
Alternative Names
CYB5B; CYB5M; OMB5; Cytochrome b5 type B; Cytochrome b5 outer mitochondrial membrane isoform
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human Cytochrome b5 type B protein (2-118AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The CYB5B Antibody (Product code: CSB-PA006310LA01HU) is Non-conjugated. For CYB5B Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA006310LB01HU CYB5B Antibody, HRP conjugated ELISA
FITC CSB-PA006310LC01HU CYB5B Antibody, FITC conjugated
Biotin CSB-PA006310LD01HU CYB5B Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function
Cytochrome b5 is a membrane-bound hemoprotein functioning as an electron carrier for several membrane-bound oxygenases.
Gene References into Functions
  1. comparison of X-ray crystal structures of human and rat CYB5B reveals a striking difference in packing involving the five-strand beta-sheet, which can be attributed to fully buried residue 21 in strand beta4 PMID: 21574570
  2. The 21 kDa protein overexpressed in Hodgkin Lymphoma and aggressive non-Hodgkin Lymphomas is identical to CYB5B. PMID: 20100355
  3. could be involved in the differential modulation of 17 alpha-hydroxylase and 17,20-lyase activities of P450c17 [type2 cyt-b5] PMID: 11867265
  4. microsomal cytochrome b5 interacts with neuronal calcium sensor protein visinin-like protein-3 in a Ca2+-dependent manner PMID: 14739275
Subcellular Location
Mitochondrion outer membrane.
Protein Families
Cytochrome b5 family
Database Links

HGNC: 24374

OMIM: 611964

KEGG: hsa:80777

STRING: 9606.ENSP00000308430

UniGene: Hs.461131

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