EP300 Antibody

Datasheet
Code CSB-PA790542
Product Type Polyclonal Antibody
Size US$297
Uniprot No. Q09472
Image
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using p300 antibody.
  • Western blot analysis of extracts from MDA-MB-435 cells, using p300 antibody.
  • Western blot analysis of extracts from HepG2 cells (Lane 2), using p300 antiobdy. The lane on the left is treated with synthesized peptide.
Immunogen Synthesized peptide derived from N-terminal of Human p300.
Raised in Rabbit
Species Reactivity Human
Specificity The antibody detects endogenous levels of total p300 protein.
Tested Applications ELISA,WB,IHC;WB:1:500-1:3000,IHC:1:50-1:100
Relevance Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac). Also functions as acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator in the presence of CREBBP. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Acetylates FOXO1 and enhances its transcriptional activity. Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity. Participates in CLOCK or NPAS2-regulated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter.

Wuchao Yuan, J. Biol. Chem., Apr 1996; 271: 9009.
E. Tory Manning, Mol. Cell. Biol., Jun 2001; 21: 3876 - 3887.
Jiwen Li, Mol. Cell. Biol., Mar 2000; 20: 2031 - 2042.
Gopalswamy Jayaraman, J. Biol. Chem., Jun 1999; 274: 17342.
Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality Polyclonal
Alias E1A-associated protein p300; EP300; P300 transcription coactivator homolog;
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Target Names EP300
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Functions as histone acetyltransferase and regulates transcription via chromatin remodeling
Involvement in disease Rubinstein-Taybi syndrome 2 (RSTS2)
Subcellular Location Cytoplasm, Nucleus, Chromosome
Database Links

HGNC: 3373

OMIM: 602700

KEGG: hsa:2033

STRING: 9606.ENSP00000263253

UniGene: Hs.517517

Pathway cAMP signaling pathway
HIF-1 signaling pathway
Jak-STAT signaling pathway
Melanogenesis
Notch signaling pathway
TGF-beta signaling pathway
Wnt signaling pathway
Cell cycle
FoxO signaling pathway
Glucagon signaling pathway

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