ESR1 Antibody

Datasheet

Code CSB-PA11399A0Rb
See More Details
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. P03372
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Image
  • Western blot
    All lanes: Estrogen receptor antibody at 7ug/ml
    Lane 1 : Hela whole cell lysate
    Lane 2 : MCF7 whole cell lysate
    Lane 3 : Rat brain tissue
    Lane 4 : Colo320 whole cell lysate
    Secondary
    Goat polyclonal to Rabbit IgG at 1/10000 dilution
    Predicted band size: 67,54,48,36 kDa
    Observed band size: 66 kDa

  • Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA11399A0Rb at dilution 1:100

  • Chromatin Immunoprecipitation MCF7(1.1*106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-ESR1 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers(CSB-PP11399HU) against the ESR1 promoter.

Immunogen Recombinant human Estrogen receptor protein (10-591AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA,WB,IHC,ChIP
Relevance Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Isoform 3 can bind to ERE and inhibit isoform 1.
Form Liquid
Conjugate Non-conjugated
Storage Buffer 0.1mol/L NaCl, 15mmol/L NaN3, PH7.2
Purification Method Caprylic Acid Ammonium Sulfate Precipitation purified
Isotype IgG
Clonality Polyclonal
Alias ER-alpha,Estradiol receptor,Nuclear receptor subfamily 3 group A member 1
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Research Area Transcription
Target Names ESR1
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Function Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1.
Involvement in disease Estrogen resistance (ESTRR)
Subcellular Location Isoform 1: Nucleus, Cytoplasm, Cell membrane, Peripheral membrane protein, Cytoplasmic side, Note=A minor fraction is associated with the inner membrane, SUBCELLULAR LOCATION: Isoform 3: Nucleus, Cytoplasm, Cell membrane, Peripheral membrane protein, Cytoplasmic side, Cell membrane, Single-pass type I membrane protein, Note=Associated with the inner membrane via palmitoylation (Probable), At least a subset exists as a transmembrane protein with a N-terminal extracellular domain, SUBCELLULAR LOCATION: Nucleus, Golgi apparatus, Cell membrane
Protein Families Nuclear hormone receptor family, NR3 subfamily
Tissue Specificity Widely expressed. Isoform 3 is not expressed in the pituitary gland.
Database Links

HGNC: 3467

OMIM: 133430

KEGG: hsa:2099

STRING: 9606.ENSP00000206249

UniGene: Hs.208124

Pathway Estrogen signaling pathway

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