Code | CSB-RA007830A118phHU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
The coding sequence for the phospho-ESR1 (S118) monoclonal antibody (isolated by immunizing animals with the synthetic phosphopeptide derived from human ESR1 around the phosphorylation site of Ser 118) was cloned into the plasmids and then transfected into cell lines for in vitro expression. The product underwent affinity-chromatography-mediated purification to get the phospho-ESR1 (S118) recombinant monoclonal antibody. This p-S118-ESR1 antibody is a rabbit IgG. It is suitable for the detection of human ESR1 phosphorylated at Ser 118 residue in ELISA and IHC applications.
ESR1 is a ligand-dependent transcription factor that affects the expression of target genes. Two transcription activation functions, AF-1 and AF-2, act in a promoter- and cell-specific manner to activate gene expression. While estrogen (E2) binding regulates the activity of AF-2, phosphorylation at several sites regulates the activity of AF-1. S118, one of these phosphorylation sites, is of special relevance because its mutation inhibits ESR1 function considerably. S118 has been demonstrated to be phosphorylated by the ERK1/2 mitogen-activated protein kinases (MAPK) and the cyclin-dependent protein kinase Cdk7 in previous research.
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